Patents by Inventor Brian L. WILLIAMSON
Brian L. WILLIAMSON has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240092883Abstract: Provided herein are processes for manufacturing recombinant ranibizumab or a ranibizumab variant that include providing a liquid comprising recombinant ranibizumab or a ranibizumab variant that is substantially free of cells; capturing the recombinant ranibizumab or the ranibizumab variant in the liquid using an affinity chromatography column, wherein the eluate of the affinity chromatography column comprises the recombinant ranibizumab or the ranibizumab variant; purifying the recombinant ranibizumab or the ranibizumab variant in the eluate of step (b) using a first cation exchange chromatography column and buffers that have a pH of about pH 5.5 to about 7.Type: ApplicationFiled: October 9, 2020Publication date: March 21, 2024Applicant: Coherus Biosciences, Inc.Inventors: John Robert Ogez, Brian L. Williamson, Nessa Mary Noone Hawkins
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Patent number: 10094809Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids and includes testosterone. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.Type: GrantFiled: October 31, 2016Date of Patent: October 9, 2018Assignee: DH Technologies Development Pte. Ltd.Inventors: Subhakar Dey, Sasi Pillai, Brian L. Williamson, Subhasish Purkayastha, Michal Weinstock
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Patent number: 9970942Abstract: Methods and systems for detecting allergens using mass spectrometry are provided herein. In some aspects, a sample can be screened for the presence or quantity of ovalbumin, lysozyme, casein (isoform S1 and S2), lactoglobulin, high and low glutens, wheat, rye, oats, barley, mustard, sesame, and various types of nuts including macadamia, pistachio, brazil, walnuts, peanuts and hazelnuts by detecting one or more peptides specific to the allergen of interest using selected MRM transitions.Type: GrantFiled: September 4, 2012Date of Patent: May 15, 2018Assignee: DH Technologies Development Pte. Ltd.Inventors: Stephen J. Lock, Subhasish Purkayastha, Brian L. Williamson, Keling Dong
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Publication number: 20170115258Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids and includes testosterone. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.Type: ApplicationFiled: October 31, 2016Publication date: April 27, 2017Inventors: Subhakar Dey, Sasi Pillai, Brian L. Williamson, Subhasish Purkayastha, Michal Weinstock
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Patent number: 9546393Abstract: A method for screening a drug for cytochrome P450 (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P450 isoform. The isoforms can be selected from 2B6, 3A4, 1A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided.Type: GrantFiled: January 22, 2015Date of Patent: January 17, 2017Assignee: DH Technologies Development Pte. Ltd.Inventors: Brian L. Williamson, Subhasish Purkayastha
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Patent number: 9500622Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.Type: GrantFiled: May 20, 2013Date of Patent: November 22, 2016Assignee: DH Technologies Development Pte. Ltd.Inventors: Subhakar Dey, Sasi Pillal, Brian L. Williamson, Subhasish Purkayastha, Michal Weinstock
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Patent number: 9257267Abstract: A composition, method, and kit for calibrating a mass spectrometer including a predetermined concentration of a calibrant having a mixture of amino acid polyethylene glycol compounds and a solvent for dissolving the calibrant. The calibrant can be used in either positive or negative ionization mode, and it can be used in calibrating an atmospheric pressure chemical ionization or an electrospray mass spectrometer. The calibrant can include a mixture to enable calibration across a broad range of masses.Type: GrantFiled: March 30, 2012Date of Patent: February 9, 2016Assignee: DH Technologies Development Pte. Ltd.Inventors: Subhakar N. Dey, Marjorie S. Minkoff, Sasi K. Pillai, Subhasish Purkayastha, Brian L. Williamson
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Publication number: 20160025741Abstract: Methods and systems for detecting allergens using mass spectrometry are provided herein. In some aspects, a sample can be screened for the presence or quantity of ovalbumin, lysozyme, casein (isoform S1 and S2), lactoglobulin, high and low glutens, wheat, rye, oats, barley, mustard, sesame, and various types of nuts including macadamia, pistachio, brazil, walnuts, peanuts and hazelnuts by detecting one or more peptides specific to the allergen of interest using selected MRM transitions.Type: ApplicationFiled: September 2, 2012Publication date: January 28, 2016Applicant: DH Technologies Development Pte. Ltd.Inventors: Stephen J. Lock, Subhasish Purkayastha, Brian L. Williamson, Keling Dong
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Patent number: 9188596Abstract: Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled m Diels Alder reagent.Type: GrantFiled: December 12, 2013Date of Patent: November 17, 2015Assignee: DH Technologies Development Pte. LtdInventors: Subhakar Dey, Sasi Pillal, Brian L. Williamson, Subhasish Purkayastha
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Publication number: 20150140600Abstract: A method for screening a drug for cytochrome P450 (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P450 isoform. The isoforms can be selected from 2B6, 3A4, 1A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided.Type: ApplicationFiled: January 22, 2015Publication date: May 21, 2015Inventors: Brian L. Williamson, Subhasish Purkayastha
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Publication number: 20150045256Abstract: A method for screening a drug for cytochrome P450 (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P450 isoform. The isoforms can be selected from 2B6, 3A4, 1A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided.Type: ApplicationFiled: August 21, 2014Publication date: February 12, 2015Inventors: Brian L. Williamson, Subhasish Purkayastha
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Patent number: 8940545Abstract: A method for screening a drug for cytochrome P450 (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P450 isoform. The isoforms can be selected from 2B6, 3A4, 1A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided.Type: GrantFiled: October 14, 2010Date of Patent: January 27, 2015Assignee: DH Technologies Development Pte. Ltd.Inventors: Brian L. Williamson, Subhasish Purkayastha
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Publication number: 20140127825Abstract: Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled m Diels Alder reagent.Type: ApplicationFiled: December 12, 2013Publication date: May 8, 2014Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.Inventors: Subhakar Dey, Sasi Pillal, Brian L. Williamson, Subhasish Purkayastha
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Publication number: 20140027625Abstract: A composition, method, and kit for calibrating a mass spectrometer including a predetermined concentration of a calibrant having a mixture of amino acid polyethylene glycol compounds and a solvent for dissolving the calibrant. The calibrant can be used in either positive or negative ionization mode, and it can be used in calibrating an atmospheric pressure chemical ionization or an electrospray mass spectrometer. The calibrant can include a mixture to enable calibration across a broad range of masses.Type: ApplicationFiled: March 30, 2012Publication date: January 30, 2014Applicant: DH Technilogies Development Pte. Ltd.Inventors: Subhakar N. Dey, Marjorie S. Minkoff, Sasi K. Pillai, Subhasish Purkayastha, Brian L. Williamson
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Publication number: 20140004616Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.Type: ApplicationFiled: May 20, 2013Publication date: January 2, 2014Applicant: DH Technologies Development Pte. Ltd.Inventors: Subhakar Dey, Sasi Pillal, Brian L. Williamson, Subhasish Purkayastha, Michal Weinstock
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Publication number: 20110091981Abstract: A method for screening a drug for cytochrome P450 (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P450 isoform. The isoforms can be selected from 2B6, 3A4, 1A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided.Type: ApplicationFiled: October 14, 2010Publication date: April 21, 2011Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.Inventors: Brian L. Williamson, Subhasish Purkayastha
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Publication number: 20110003395Abstract: Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.Type: ApplicationFiled: June 1, 2010Publication date: January 6, 2011Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.Inventors: Subhakar DEY, Sasi PILLAI, Brian L. WILLIAMSON, Subhasish PURKAYASTHA, Michal Weinstock