Abstract: Provided herein are genetically engineered mammalian (e.g., human) cells that express one or more transgenes at a sustained expression level. Also provided are methods of making and using the cells.

Abstract: Strategies, systems, compositions, and methods for efficient production of knock-in cellular clones without reporter genes. An essential gene is targeted using a knock-in cassette that comprises an exogenous coding sequence for a gene product of interest (or “cargo sequence”) in frame with and downstream (3?) of an exogenous coding sequence or partial coding sequence of the essential gene. Undesired targeting events create a non-functional version of the essential gene, in essence a knock-out, which is “rescued” by correct integration of the knock-in cassette, which restores the essential gene coding region so that a functional gene product is produced and positions the cargo sequence in frame with and downstream of the essential gene coding sequence.

Abstract: A network-based method and system for distributing data, such as service data for various types of service processes and models, including software applications, specifications, user's manuals, or parameters, etc, over a data transmission network. A remote service provider maintains a database of the data. The database is updated frequently. The remote service provider maintains a website for authorized users to access the data. Authorized users can access and download desired data by connecting to the remote service provider via the data transmission network. Certain approaches are used to verify a user's identity. In one aspect, a combination of a product code that is specific to a system and a license/activation code that is generated based on the product code is verified. Unless a proper combination exists, the downloaded data cannot be properly accessed. The remote service provider also allows users to purchase a license to access the data online.

Abstract: A network-based method and system for distributing data, such as service data for various types of service processes and models, including software applications, specifications, user's manuals, or parameters, etc, over a data transmission network. A remote service provider maintains a database of the data. The database is updated frequently. The remote service provider maintains a website for authorized users to access the data. Authorized users can access and download desired data by connecting to the remote service provider via the data transmission network. Certain approaches are used to verify a user's identity. In one aspect, a combination of a product code that is specific to a system and a license/activation code that is generated based on the product code is verified. Unless a proper combination exists, the downloaded data cannot be properly accessed. The remote service provider also allows users to purchase a license to access the data online.