Patents by Inventor Brian R. Scott
Brian R. Scott has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9279112Abstract: Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acid sequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolyzing cellulose with the modified cellulases in the presence of lignin.Type: GrantFiled: February 26, 2010Date of Patent: March 8, 2016Assignee: IOGEN ENERGY CORPORATIONInventors: Brian R. Scott, Patrick St-Pierre, James Lavigne, Nabil Masri, Theresa C. White, John J. Tomashek
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Patent number: 9206406Abstract: Provided is a modified Family 1 carbohydrate binding module (CBM) comprising amino acid substitutions at one or more of positions 10, 11, 12, 14, 17, 21, 24, 29, 31, 33, and 37, said position determined from alignment of a Family 1 CBM amino acid sequence with SEQ ID NO: 30, and exhibiting from about 50% to about 99.9% amino acid sequence identity to SEQ ID NO: 30. Also provided are modified glycosidase enzymes comprising the modified Family 1 CBM, genetic constructs and genetically modified microbes for expressing the modified Family 1 CBM or modified glycosidase enzyme. The modified Family 1 CBM confers reduced lignin binding and/or increased hydrolyzing activity in the presence of lignin to the modified glycosidase enzyme, which may be used in a process for hydrolyzing cellulose or hemicellulose in the presence of lignin.Type: GrantFiled: February 11, 2011Date of Patent: December 8, 2015Assignee: IOGEN ENERGY CORPORATIONInventors: John J. Tomashek, Brian R. Scott, Daniel Kolczynski
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Publication number: 20140356914Abstract: The present invention provides endoglucanase 1b (EG1b) suitable for use in saccharification reactions.Type: ApplicationFiled: August 27, 2012Publication date: December 4, 2014Applicant: Codexis, Inc.Inventors: Kripa Rao, Xiyun Zhang, Brian R. Scott, John J. Tomashek
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Publication number: 20130344541Abstract: The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures.Type: ApplicationFiled: November 1, 2011Publication date: December 26, 2013Applicant: CODEXIS, INC.Inventors: Ish Kumar Dhawan, Dipnath Baidyaroy, Andrew Shaw, Oleh Tanchak, Christopher Hill, Chengsong Liu, Amala Chokshi, Brian R. Scott
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Patent number: 8486683Abstract: Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improvements in one or more kinetic parameters (KG, KG2, kcat) comprising amino acid substitutions at one or more of positions 43, 101, 260 and 543. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.Type: GrantFiled: May 28, 2010Date of Patent: July 16, 2013Assignee: Iogen Energy CorporationInventors: Brian R. Scott, Chengsong Liu, James Lavigne, John J. Tomashek
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Publication number: 20120295308Abstract: Provided is a modified Family 1 carbohydrate binding module (CBM) comprising amino acid substitutions at one or more of positions 10, 11, 12, 14, 17, 21, 24, 29, 31, 33, and 37, said position determined from alignment of a Family 1 CBM amino acid sequence with SEQ ID NO: 30, and exhibiting from about 50% to about 99.9% amino acid sequence identity to SEQ ID NO: 30. Also provided are modified glycosidase enzymes comprising the modified Family 1 CBM, genetic constructs and genetically modified microbes for expressing the modified Family 1 CBM or modified glycosidase enzyme. The modified Family 1 CBM confers reduced lignin binding and/or increased hydrolysing activity in the presence of lignin to the modified glycosidase enzyme, which may be used in a process for hydrolysing cellulose or hemicellulose in the presence of lignin.Type: ApplicationFiled: February 11, 2011Publication date: November 22, 2012Applicant: IOGEN ENEGY CORPORATIONInventors: John J. Tomashek, Brian R. Scott, Daniel Kolczynski
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Patent number: 8309328Abstract: The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures.Type: GrantFiled: July 2, 2012Date of Patent: November 13, 2012Assignee: Codexis, Inc.Inventors: Ish Kumar Dhawan, Dipnath Baidyaroy, Andrew Shaw, Oleh Tanchak, Christopher Hill, Chengsong Liu, Amala Chokshi, Brian R. Scott
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Publication number: 20120270278Abstract: The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures.Type: ApplicationFiled: July 2, 2012Publication date: October 25, 2012Applicant: CODEXIS, INC.Inventors: ISH KUMAR DHAWAN, DIPNATH BAIDYAROY, ANDREW SHAW, OLEH TANCHAK, CHRISTOPHER HILL, CHENGSONG LIU, AMALA CHOKSHI, BRIAN R. SCOTT
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Patent number: 8236551Abstract: The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures.Type: GrantFiled: November 1, 2011Date of Patent: August 7, 2012Assignee: Codexis, Inc.Inventors: Ish Kumar Dhawan, Dipnath Baidyaroy, Andrew Shaw, Oleh Tanchak, Christopher Hill, Chengsong Liu, Amala Chokshi, Brian R. Scott
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Publication number: 20120107881Abstract: The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures.Type: ApplicationFiled: November 1, 2011Publication date: May 3, 2012Applicant: CODEXIS, INC.Inventors: Ish Kumar Dhawan, Dipnath Baidyaroy, Andrew Shaw, Oleh Tanchak, Christopher Hill, Chengsong Liu, Amala Chokshi, Brian R. Scott
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Patent number: 8110389Abstract: A modified Trichoderma reesei Family 6 (TrCel6A) cellulase enzyme comprising amino acid substitutions at one or more positions selected from the group consisting of 129, 322, 363 and 410 of SEQ ID NO: 1 is provided. Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified TrCel6a cellulase are also provided. The modified TrCel6A cellulase of the invention display at least a 15% decrease in inactivation by lignin relative to a parental TrCel6A cellulase from which the modified TrCel6A is derived. Such cellulases find use in a variety of applications in industry requiring enzymatic hydrolysis of cellulose in the presence of lignin, e.g., the hydrolysis of pretreated lignocellulosic feedstocks for the production of fermentable sugars, sugar alcohols and fuel alcohols.Type: GrantFiled: July 30, 2009Date of Patent: February 7, 2012Assignee: Iogen Energy CorporationInventors: James A. Lavigne, Brian R. Scott, Martine Whissel, John J. Tomashek
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Patent number: 8017361Abstract: Provided is an enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars. The enzyme mixture comprises EG4 at a fractional concentration (fEG4) of about 0.25 to about 0.83 (w/w), Swollenin at a fractional concentration (fSwo1) of about 0 to about 0.66 (w/w), and Cip1 at a fractional concentration (fCip1) of 0 to about 0.33 measured relative to all accessory enzymes present in the enzyme mixture. Also provided are processes for converting a pretreated lignocellulosic feedstock to soluble sugars using the enzyme mixtures, and methods of using and producing such enzyme mixtures.Type: GrantFiled: August 29, 2008Date of Patent: September 13, 2011Assignee: Iogen Energy CorporationInventors: Brian R. Scott, Christopher Hill, John Tomashek, Chengsong Liu
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Publication number: 20100304438Abstract: Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improvements in one or more kinetic parameters (KG, KG2, kcat) comprising amino acid substitutions at one or more of positions 43, 101, 260 and 543. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.Type: ApplicationFiled: May 28, 2010Publication date: December 2, 2010Applicant: Iogen Energy CorporationInventors: Brian R. Scott, Chengsong Liu, James Lavigne, John J. Tomashek
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Publication number: 20100221778Abstract: Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acidsequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolysing cellulose with the modified cellulases in the presence of lignin.Type: ApplicationFiled: February 26, 2010Publication date: September 2, 2010Applicant: IOGEN ENERGY CORPORATIONInventors: Brian R. Scott, Patrick St-Pierre, James Lavigne, Nabil Masri, Theresa C. White, John J. Tomashek
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Patent number: 7703375Abstract: A composite armor 10 is provided with a cellular structure 12 having a plurality of composite inserts 14 arranged in openings 20 provided in the cellular structure 12. A pair of laminate sheets are arranged on oppositely facing sides of the cellular structure 12 and covering the inserts 14. The laminate sheets 26 and 28 are bonded to the structure 12 and the inserts 14 by an adhesive. The cellular structure 12 includes a plurality of fibers extending continuously throughout the cellular structure 12.Type: GrantFiled: August 15, 2006Date of Patent: April 27, 2010Assignee: Lawrence Technological UniversityInventors: Brian R. Scott, Nabil F. Grace
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Publication number: 20100089228Abstract: A composite armor 10 is provided with a cellular structure 12 having a plurality of composite inserts 14 arranged in openings 20 provided in the cellular structure 12. A pair of laminate sheets are arranged on oppositely facing sides of the cellular structure 12 and covering the inserts 14. The laminate sheets 26 and 28 are bonded to the structure 12 and the inserts 14 by an adhesive. The cellular structure 12 includes a plurality of fibers extending continuously throughout the cellular structure 12.Type: ApplicationFiled: August 15, 2006Publication date: April 15, 2010Inventors: Brian R. Scott, Nabil F. Grace
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Publication number: 20100041100Abstract: A modified Trichoderma reesei Family 6 (TrCel6A) cellulase enzyme comprising amino acid substitutions at one or more positions selected from the group consisting of 129, 322, 363 and 410 of SEQ ID NO: 1 is provided. Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified TrCel6a cellulase are also provided. The modified TrCel6A cellulase of the invention display at least a 15% decrease in inactivation by lignin relative to a parental TrCel6A cellulase from which the modified TrCel6A is derived. Such cellulases find use in a variety of applications in industry requiring enzymatic hydrolysis of cellulose in the presence of lignin, e.g., the hydrolysis of pretreated lignocellulosic feedstocks for the production of fermentable sugars, sugar alcohols and fuel alcohols.Type: ApplicationFiled: July 30, 2009Publication date: February 18, 2010Applicant: IOGEN ENERGY CORPORATIONInventors: James A. Lavigne, Brian R. Scott, Martine Whissel, John J. Tomashek
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Publication number: 20090061484Abstract: Provided is an enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars. The enzyme mixture comprises EG4 at a fractional concentration (fEG4) of about 0.25 to about 0.83 (w/w), Swollenin at a fractional concentration (fSwo1) of about 0 to about 0.66 (w/w), and Cip1 at a fractional concentration (fCip1) of 0 to about 0.33 measured relative to all accessory enzymes present in the enzyme mixture. Also provided are processes for converting a pretreated lignocellulosic feedstock to soluble sugars using the enzyme mixtures, and methods of using and producing such enzyme mixtures.Type: ApplicationFiled: August 29, 2008Publication date: March 5, 2009Applicant: IOGEN ENERGY CORPORATIONInventors: Brian R. Scott, Christopher Hill, John Tomashek, Chengsong Liu