Patents by Inventor Bruce P. Neri
Bruce P. Neri has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 7432048Abstract: The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5? nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: December 8, 2000Date of Patent: October 7, 2008Assignee: Third Wave Technologies, Inc.Inventors: Bruce P. Neri, Jeff G. Hall, Victor Lyamichev, Lloyd M. Smith
-
Patent number: 7429455Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).Type: GrantFiled: September 5, 2006Date of Patent: September 30, 2008Assignee: Third Wave Technologies, Inc.Inventors: Fang Dong, Victor I. Lyamichev, James R. Prudent, Lance Fors, Bruce P. Neri, Mary Ann D. Brow, Todd A. Anderson, James E. Dahlberg
-
Publication number: 20080188375Abstract: The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5? nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: ApplicationFiled: October 28, 2007Publication date: August 7, 2008Applicant: THIRD WAVE TECHNOLOGIES, INC.Inventors: Bruce P. Neri, Jeff G. Hall, Victor I. Lyamichev, Lloyd M. Smith
-
Publication number: 20080187926Abstract: The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to improved methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.Type: ApplicationFiled: October 30, 2007Publication date: August 7, 2008Applicant: THIRD WAVE TECHNOLOGIES, INC.Inventors: James E. Dahlberg, Hatim T. Allawi, Victor Lyamichev, Bruce P. Neri, Marilyn C. Olson-Munoz, LuAnne Chehak, Sarah M. Olson
-
Patent number: 7384746Abstract: The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.Type: GrantFiled: June 23, 2004Date of Patent: June 10, 2008Assignee: Third Wave Technologies, Inc.Inventors: Victor Lyamichev, Zbigniev Skrzypczynski, Hatim T. Allawi, Sarah R. Wayland, Tsetska Y. Takova, Bruce P. Neri, James R. Prudent, Jeff G. Hall
-
Patent number: 7303869Abstract: The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5? nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.Type: GrantFiled: October 11, 2002Date of Patent: December 4, 2007Assignee: Northwestern UniversityInventors: Priscilla Wilkins Stevens, David Kelso, Victor Lyamichev, Jeff Hall, Bruce P. Neri, Robert W. Kwiatkowski, Jr., Lloyd M. Smith
-
Patent number: 7256020Abstract: The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5? nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: February 3, 2003Date of Patent: August 14, 2007Assignee: Third Wave Technologies, Inc.Inventors: Victor Lyamichev, Bruce P. Neri, Jeff Hall, Andrew Lukowiak
-
Patent number: 7195871Abstract: The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5? nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: November 7, 2002Date of Patent: March 27, 2007Assignee: Third Wave Technologies, IncInventors: Victor Lyamichev, Bruce P. Neri, Jeff Hall, Andrew A. Lukowiak
-
Patent number: 7150982Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: February 26, 2002Date of Patent: December 19, 2006Assignee: Third Wave Technologies, Inc.Inventors: Hatim T. Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael W. Kaiser, Robert W. Kwiatkowski, Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
-
Patent number: 7101672Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acids and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).Type: GrantFiled: September 4, 2003Date of Patent: September 5, 2006Assignee: Third Wave Technologies, Inc.Inventors: Fang Dong, Victor I. Lyamichev, James R. Prudent, Lance Fors, Bruce P. Neri, Mary Ann D. Brow, Todd A. Anderson, James E. Dahlberg
-
Patent number: 7060436Abstract: The present invention relates to methods and compositions for analyzing nucleic acids, and in particular, methods and compositions for detection and characterization of nucleic acid sequences and sequence changes. The present invention also provides methods and compositions for identifying oligonucleotides with desired hybridization properties to nucleic acid targets containing secondary structure.Type: GrantFiled: June 15, 2001Date of Patent: June 13, 2006Assignee: Third Wave Technologies, Inc.Inventors: Victor Lyamichev, Hatim Allawi, Fang Dong, Bruce P. Neri, Tatiani I. Vener
-
Patent number: 7045289Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: May 24, 2001Date of Patent: May 16, 2006Assignee: Third Wave Technologies, Inc.Inventors: Hatim Allawi, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Michael Kaiser, Robert W. Kwiatkowski, Jr., Andrew A. Lukowiak, Victor Lyamichev, WuPo Ma, Marilyn C. Olson-Munoz, Sarah M. Olson, James J. Schaefer, Zbigniew Skrzypczynski, Tsetska Y. Takova, Kevin L. Vedvik, Natalie Lyamichev, Bruce P. Neri
-
Publication number: 20040235024Abstract: The present invention relates to methods and compositions for analyzing nucleic acids, and in particular, methods and compositions for detection and characterization of nucleic acid sequences and sequence changes. The present invention also provides methods and compositions for identifying oligonucleotides with desired hybridization properties to nucleic acid targets containing secondary structure.Type: ApplicationFiled: March 23, 2004Publication date: November 25, 2004Inventors: Victor Lyamichev, Hatim Allawi, Fang Dong, Bruce P. Neri, Tatiana I. Vener
-
Publication number: 20040214174Abstract: The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: ApplicationFiled: December 4, 2002Publication date: October 28, 2004Inventors: Bruce P. Neri, Jeff G. Hall, Victor Lyamichev, Lloyd M. Smith
-
Patent number: 6780982Abstract: The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.Type: GrantFiled: February 6, 2001Date of Patent: August 24, 2004Assignee: Third Wave Technologies, Inc.Inventors: Victor Lyamichev, Zbigniew Skrzpczynski, Hatim T. Allawi, Sarah R. Wayland, Tsetska Takova, Bruce P. Neri
-
Publication number: 20040161749Abstract: The present invention provides compositions comprising oligonucleotides that have 3′ end groups (e.g. lipophilic moieties) that are useful in invasive cleavage reactions such as the INVADER assay. Specifically, the present invention provides compositions containing oligonucleotides with 3′ end groups configured for generating a detectable signal in invasive cleavages assays with a high signal-to-background ratio, as well as methods for generating such compositions.Type: ApplicationFiled: January 24, 2003Publication date: August 19, 2004Inventors: Jeff G. Hall, Zbigniev Skrzypczynski, Sarah Wayland, Ned D. Reimer, Luis P. Reynaldo, Joerg Baier, Victor Lyamichev, Bruce P. Neri
-
Patent number: 6759226Abstract: The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.Type: GrantFiled: May 24, 2000Date of Patent: July 6, 2004Assignee: Third Wave Technologies, Inc.Inventors: Wu-Po Ma, Victor I. Lyamichev, Michael W. Kaiser, Natalie E. Lyamicheva, Hatim Taysir Allawi, James J. Schaefer, Bruce P. Neri
-
Publication number: 20040072182Abstract: The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: ApplicationFiled: February 3, 2003Publication date: April 15, 2004Inventors: Victor Lyamichev, Bruce P. Neri, Jeff Hall, Andrew Lukowiak
-
Patent number: 6709819Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).Type: GrantFiled: April 3, 2001Date of Patent: March 23, 2004Assignee: Third Wave Technologies, Inc.Inventors: Victor I. Lyamichev, Fang Dong, Mary Ann D. Brow, Lance Fors, Bruce P. Neri
-
Patent number: 6709815Abstract: The present invention relates to methods and compns. for treating nucleic acids, and in particular, methods and compns. for the detection and characterization of nucleic acid sequences and sequence changes. The invention provides methods for examg. the conformations assumed by single strands of nucleic acid, forming the basis of novel methods of detection of specific nucleic acid sequences. The present invention contemplates use of novel detection methods for, among other uses, clinical diagnostic purposes, including but not limited to the detection and identification of pathogenic organisms. Examples are presented for the analysis of Mycobacterium tuberculosis and hepatitis C virus genes.Type: GrantFiled: July 18, 2000Date of Patent: March 23, 2004Assignee: Third Wave Technologies, Inc.Inventors: Fang Dong, Victor I. Lyamichev, James R. Prudent, Lance Fors, Bruce P. Neri, Mary Ann D. Brow, Todd A. Anderson, James E. Dahlberg