Abstract: Supplemented mixotrophic method. A mixotrophic fermentation method is disclosed including providing a naturally acetogenic organism; providing a fermentation medium comprising a carbon source and a supplemented non-sugar reductant; and culturing the organism in the fermentation medium, where both the carbon source and the non-sugar reductant are metabolized and a fermentation broth is formed, which contains at least one carbon-containing bioproduct.

Abstract: Methods for producing a bioproduct selected from acetone, isopropanol and a combination thereof with a microorganism in a fermentor are disclosed. The methods include separating cells of the microorganism from a fermentation broth to form separated cells and recycling at least a fraction of the separated cells to the fermentor to achieve one or more of the following: (1) cell concentration in said fermentor greater than 2 g/L; mass yield on a first feedstock greater than 32%; productivity greater than 0.12 g/L/h; and bioproduct titer greater than 10 g/L.

Abstract: Integrated mixotrophic fermentation method comprising (i) an isolated naturally acetogenic organism; (ii) a first feedstock comprising a carbon source for use in a fermentation medium; (iii) a second feedstock comprising elemental hydrogen for use in the fermentation medium; wherein the second feedstock comprises performing electrolysis; and (iv) culturing the organism in the fermentation medium, whereby both feedstocks are metabolized and a fermentation broth is formed, which broth comprises at least one bioproduct.

Abstract: Supplemented mixotrophic method. A mixotrophic fermentation method is disclosed including providing a naturally acetogenic organism; providing a fermentation medium comprising a carbon source and a supplemented non-sugar reductant; and culturing the organism in the fermentation medium, where both the carbon source and the non-sugar reductant are metabolized and a fermentation broth is formed, which contains at least one carbon-containing bioproduct.

Abstract: High cell density anaerobic fermentation for protein expression. A method for producing a protein is described, including providing a modified Butyribacterium methylotrophicum (Bme) strain host; providing a fermentation medium including a carbon source and a nitrogen source; culturing the modified host in the fermentation medium under anaerobic conditions, where the carbon source is metabolized and a fermentation broth including at least one protein is formed and optionally separating the protein; where the Bme cell density in the fermentation broth is greater than 15 g/L.

Abstract: Integrated mixotrophic fermentation method comprising (i) an isolated naturally acetogenic organism; (ii) a first feedstock comprising a carbon source for use in a fermentation medium; (iii) a second feedstock comprising elemental hydrogen for use in the fermentation medium; wherein the second feedstock comprises performing electrolysis; and (iv) culturing the organism in the fermentation medium, whereby both feedstocks are metabolized and a fermentation broth is formed, which broth comprises at least one bioproduct.

Abstract: Methods for producing a bioproduct selected from acetone, isopropanol and a combination thereof with a microorganism in a fermentor are disclosed. The methods include separating cells of the microorganism from a fermentation broth to form separated cells and recycling at least a fraction of the separated cells to the fermentor to achieve one or more of the following: (1) cell concentration in said fermentor greater than 2 g/L; mass yield on a first feedstock greater than 32%; productivity greater than 0.12 g/L/h; and bioproduct titer greater than 10 g/L.