Abstract: Lower eukaryote host cells in which an endogenous or heterologous Ca2+ ATPase is overexpressed are described. Also described are lower eukaryote host cells in which a calreticulin and/or ERp57 protein are overexpressed. These host cells are useful for producing recombinant glycoproteins that have reduced O-glycosylation.

Abstract: A voice communication method and system that enables establishing a voice communication in a URC environment is provided. The voice communication method and system of the present invention allow establishing a voice communication channel between terminals (private IP address to private IP address, public IP address to public IP address, and private IP address to public IP address) in a URC environment. Particularly, the voice communication system of the present invention is implemented with a call server acting as a STUN server for supporting voice communication between two terminals of which one is assigned private IP address and controlling the communication session in the URC environment.

Abstract: The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells exhibit GnTIII activity, which produce bisected N-glycan structures and may be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

Abstract: The present invention is related to methods and for producing higher titers of recombinant protein in a modified yeast host cell, for example Pichia pastoris, wherein the modified yeast cell lacks vacuolar sorting activity or has decreased vacuolar sorting activity relative to an unmodified yeast host cell of the same species. In particular embodiments vacuolar sorting activity is reduced or eliminated by deletion or disruption of a gene encoding Vps10 or a Vps10 homolog. The invention is also related to the modified yeast cells which are modified in accordance with the methods disclosed herein.

Abstract: Described is a method for increasing the N-glycosylation site occupancy of a therapeutic glycoprotein produced in recombinant host cells modified as described herein and genetically engineered to express the glycoprotein compared to the N-glycosylation site occupancy of the therapeutic glycoprotein produced in a recombinant host cell not modified as described herein. In particular, the method provides recombinant host cells that overexpress a heterologous single-subunit oligosaccharyltransferase, which in particular embodiments is capable of functionally suppressing the lethal phenotype of a mutation of at least one essential protein of the yeast oligosaccharyltransferase (OTase) complex, for example, the Leishmania major STT3D protein, in the presence of expression of the host cell genes encoding the endogenous OTase complex.

Abstract: The present invention relates to a switch control circuit, a switch control method, and a converter using the same. An input voltage rectified from an AC input in a converter is transmitted to an inductor, and output power is generated from an inductor current by the input voltage. The converter includes a power switch connected to the inductor to control the inductor current and a sense resistor having a first end connected to a ground and a second end connected to the AC input. At a time point that a sense voltage generated in the sense voltage reaches the peak point and then decreased to an on-reference voltage, the power switch is turned on. The on-reference voltage is a sense voltage at a resonance start time point between a parasite capacitor of the power switch and the inductor.

Abstract: The present invention relates to a switch control circuit, a switch control method, and a converter using the same. An input voltage of a converter is provided to an inductor, and an output voltage is generated by an inductor current caused by the input voltage. A switch control circuit for controlling a switching operation of a power switch connected to the inductor to control the inductor current senses a drain current flowing to the power switch while the power switch is turned on, and controls a slope of a sawtooth wave signal for determining a turn-off time of the power switch according to the sensed drain current.

Abstract: The present invention provides Herpes Simplex Virus (HSV) gD, gC, gB and/or gE recombinant glycoproteins having a particular pre-selected N-linked glycosylation pattern as the predominant N-glycoform. The present invention also provides methods of producing these recombinant glycoproteins in yeast, preferably Pichia pastoris, which may be glycoengineered to provide particular glycosylation patterns. The present invention further provides vaccines comprising gD and gC, and optionally gB and/or gE, at least one of which has a particular pre-selected N-linked glycosylation pattern as the predominant N-glycoform. The recombinant glycoproteins are produced by a method which, in one embodiment, comprises transforming a yeast of the genus Pichia with an expression vector containing a DNA encoding an HSV glycoprotein, which is under regulation of a promoter functional in a yeast of the genus Pichia, culturing the yeast in a medium, and recovering the recombinant glycoprotein from the obtained culture.

Abstract: The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g.

Abstract: Lower eukaryote host cells in which the function of at least one endogenous gene encoding a chaperone protein, such as a Protein Disulphide Isomerase (PDI), has been reduced or eliminated and at least one mammalian homolog of the chaperone protein is expressed are described. In particular aspects, the host cells further include a deletion or disruption of one or more O-protein mannosyltransferase genes, and/or overexpression of an endogenous or exogenous Ca2+ATPase. These host cells are useful for producing recombinant glycoproteins in large amounts and for producing recombinant glycoproteins that have reduced O-glycosylation.

Abstract: A system to provide various content services based on a network to users includes a service provider to provide a service using the network, a user to receive the service, and a service framework to provide the service to the user through the network. The service framework includes a messenger device that manages the service transmitted between the service provider and the user. Content services, which are being provided based on a network, can be provided to various terminals including not only a PC and a mobile phone, but also a robot. Thus, extending services is easy and the user can conveniently receive desired information from a knowledge provider or website without performing repetitive operations.

Abstract: The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g.

Abstract: A client device, a control method thereof, and an image service system including the client service and the control method are disclosed. The client device includes an image capturing unit to capture an image, an encoding unit to encode the image captured by the image capturing unit into a JPEG format according to a group map that includes not only Frames Per Second (FPS) values required for types of a plurality of image services but also compression information for each frame image, and a client controller to determine not only the FPS satisfying the plurality of image services but also the compression information for each frame image, and control an operation of the encoding unit in such a manner that images captured by the image capturing unit are encoded according to the determined FPS and compression information.

Abstract: Lower eukaryote host cells in which an endogenous or heterologous Ca2+ ATPase is overexpressed are described. Also described are lower eukaryote host cells in which a calreticulin and/or ERp57 protein are overexpressed. These host cells are useful for producing recombinant glycoproteins that have reduced O-glycosylation.

Abstract: The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g.

Abstract: The present invention relates to a power factor correction circuit and a driving method thereof. The power factor correction circuit includes a power switch controlling an inductor current flowing in an inductor, an auxiliary inductor coupled to the inductor with a predetermined turn ratio, and a power factor correction controller controlling output power by controlling a switching operation of the power switch. The power factor correction controller determines whether or not an output voltage of the output power is an over-voltage by using the sum of a source current and a sink current that control a zero current detection voltage to be included within a predetermined clamping range, the zero current detection voltage corresponding to an auxiliary voltage that is a both-end voltage of an auxiliary inductor.

Abstract: Disclosed herein is a system which transmits an image using a lossless compression method in a robot to provide a service over a network and a method thereof. The network-based robot separates an image acquired by a stereo camera into various image formats and transmits the various image formats to a service server. The service server synthesizes the separated image formats to suit an image request such as face recognition, object recognition, navigation or monitoring to restore and provide an original image. When the network-based robot transmits the separated image formats to the server, the original image is transmitted using the lossless method to improve the performance of the server. Even when a service using a new image is added, separated images are transmitted with respect to the image format requested by this service to more flexibly cope with the service using the new image. Since channels are separated in order to receive lossless data, network gain is obtained.

Abstract: The present invention provides a novel lower eukaryotic host cell producing human-like glycoproteins characterized as having a terminal ?-galactose residue and essentially lacking fucose and sialic acid residues. The present invention also provides a method for catalyzing the transfer of a galactose residue from UDP-galactose onto an acceptor substrate in a recombinant lower eukaryotic host cell, which can be used as a therapeutic glycoprotein.

Abstract: A software robot apparatus (sobot apparatus) with a genetic code is disclosed. The sobot apparatus includes an application module, an artificial animated object engine, a sobot protocol module, an update agent, an update protocol module, a network interface, a sobot player, a script engine, a player database, a repository interface, a sobot graphic/audio interface, a 3D engine, a system software, a memory device, a display part, and an audio output part.

Abstract: Provided are a network-based robot control system and a robot velocity control method in the network-based robot control system. A client calculates a robot control velocity according to its reception state of video data frames captured by a robot, generates a robot control message including the calculated robot control velocity, and transmits the robot control message to the robot. The robot then changes its velocity according to the robot control velocity included in the received robot control message. In this way, the velocity of the robot is controlled according to the video data reception state of the client, thereby allowing a user to easily control the robot regardless of the performance of the client.