Abstract: The present invention provides compositions and methods for regulating expression of nucleotide sequences in fungi. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the Agaricus bisporus lectin gene. The sequences drive expression preferentially to fruit body tissue. A method for expressing a nucleotide sequence in fungi using the regulatory sequences disclosed herein is provided. The method comprises transforming a fungal cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed fungus from the transformed cell.

Abstract: The present invention provides compositions and methods for regulating expression of nucleotide sequences in fungi. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the Agaricus bisporus lectin gene. The sequences drive expression preferentially to fruit body tissue. A method for expressing a nucleotide sequence in fungi using the regulatory sequences disclosed herein is provided. The method comprises transforming a fungal cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed fungus from the transformed cell.

Abstract: The present invention provides compositions and methods for regulating expression of nucleotide sequences in fungi. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the Agaricus bisporus lectin gene. The sequences drive expression preferentially to fruit body tissue. A method for expressing a nucleotide sequence in fungi using the regulatory sequences disclosed herein is provided. The method comprises transforming a fungal cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed fungus from the transformed cell.

Abstract: The present invention provides compositions and methods for regulating expression of nucleotide sequences in fungi. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the Agaricus bisporus lectin gene. The sequences drive expression preferentially to fruit body tissue. A method for expressing a nucleotide sequence in fungi using the regulatory sequences disclosed herein is provided. The method comprises transforming a fungal cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed fungus from the transformed cell.

Abstract: Disclosed is a new technology that enables the transgenic modification of a mushroom-forming fungus to confer a transgenic genotype and/or phenotype. According to the invention herein, one can manipulate the fruiting body of a fungus conferring an altered phenotype, but a wild-type genotype. The fruiting body itself is devoid of any cognate transgene, and Applicants have discovered a new mechanism of protein synthesis and accumulation in fungi and a technique to independently control the genotype and phenotype of the fruiting body.

Abstract: Disclosed is a new technology that enables the transgenic modification of a mushroom-forming fungus to confer a transgenic genotype and/or phenotype. According to the invention herein, one can manipulate the fruiting body of a fungus conferring an altered phenotype, but a wild-type genotype. The fruiting body itself is devoid of any cognate transgene, and Applicants have discovered a new mechanism of protein synthesis and accumulation in fungi and a technique to independently control the genotype and phenotype of the fruiting body.

Abstract: The present invention provides compositions and methods for regulating expression of nucleotide sequences in fungi. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the Agaricus bisporus lectin gene. The sequences drive expression preferentially to fruit body tissue. A method for expressing a nucleotide sequence in fungi using the regulatory sequences disclosed herein is provided. The method comprises transforming a fungal cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed fungus from the transformed cell.

Abstract: Applicants have devised a highly effective, convenient, and expeditious genetic transformation system for filamentous fungi, such as Agaricus bisporus. The preferred method uses an Agrobacterium-mediated transformation protocol. The critical features of this protocol include co-cultivation of the bacterium with fruit body tissue instead of spores. In a preferred embodiment, even higher transformation efficiencies were observed with the use of a homologous promoter in the polynucleotide expression construct in order to drive gene expression.

Abstract: Applicants have devised a highly effective, convenient, and expeditious genetic transformation system for filamentous fungi, such as Agaricus bisporus. The preferred method uses an Agrobacterium-mediated transformation protocol. The critical features of this protocol include co-cultivation of the bacterium with fruit body tissue instead of spores. In a preferred embodiment, even higher transformation efficiencies were observed with the use of a homologous promoter in the polynucleotide expression construct in order to drive gene expression.

Abstract: Applicants have devised a highly effective, convenient, and expeditious genetic transformation system for filamentous fungi, such as Agaricus bisporus. The preferred method uses an Agrobacterium-mediated transformation protocol. The critical features of this protocol include co-cultivation of the bacterium with fruit body tissue instead of spores. In a preferred embodiment, even higher transformation efficiencies were observed with the use of a homologous promoter in the polynucleotide expression construct in order to drive gene expression.

Abstract: This invention provides methods and reagents for screening plants, particularly geraniums, for infections with bacterial pathogens. Specifically, the invention provides nucleic acid amplification and detection methods for specifically detecting a certain pathovar of a bacterial infectious agent in geraniums. The invention provides a nucleic acid hybridization probe that is specific for Xanthomonas campestris pathovar pelargonii, and paired sets of oligonucleotide primers for polymerase chain reaction-catalyzed amplification of DNA fragments comprising the nucleic acid sequences of the invention specific for Xanthomonas campestris pathovar pelargonii template DNA. The invention also provides PCR-based methods for detecting Xanthomonas campestris pathovar pelargonii DNA, and in particular for distinguishing pathovar DNA from the DNA of the host infected plant. Methods for producing detectably-labeled hybridization probes using PCR are also provided.

Abstract: There is provided a method of cultivating mushrooms and mushroom spawn, wherein a delayed-release nutrient supplement is utilized. The method is an improvement over prior methods which utilize nutrient supplements in the growing of mushrooms and mushroom spawn. The improvement comprises using as a nutrient supplement an intact seed having its sprouting capability irreversibly inhibited or destroyed, or mixtures thereof, and having inherent delayed-release properties.

Abstract: Intact seed of rapeseed is added to compost at spawning to increase the yield of mushrooms similar to chemical-based delayed-release supplements. No chemicals, however, are needed in its preparation, only a treatment to destroy sprouting of the seed.

Abstract: A synthetic substrate which supports the growth and development of filamentous fungi is disclosed. The substrate is comprised of a nutrient in a hydrated hydrogel matrix forming a capsule. In a preferred embodiment, the capsule has an irregular external surface to allow filamentous fungi to adhere thereto even when shaken. Also disclosed is a synthetic CACing agent which includes the synthetic substrate of the present invention, a method of manufacturing the synthetic substrate of the present invention and a method of cultivating mushroom spawn and cultivating mushrooms utilizing the synthetic substrate.