Patents by Inventor Camilla Estmer-Nilsson
Camilla Estmer-Nilsson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240076605Abstract: The present invention relates to a method for controlling process parameters in a bioreactor when producing a target product with a pre-determined critical to quality, CTQ, profile. The method comprises: monitoring (S20) at least one product quality attribute, PQA, for the target product; identifying (S30) trends and/or deviations by comparing the monitored at least one PQA to the CTQ profile of the target product; and controlling (S40) process parameters of the bioreactor based on the identified trends and/or deviations to maintain the target product within the CTQ profile.Type: ApplicationFiled: February 1, 2022Publication date: March 7, 2024Applicant: CYTIVA SWEDEN ABInventors: ANDREAS AXÉN, HELENA VESTERBERG, MAGNUS WETTERHALL, CAMILLA ESTMER NILSSON
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Publication number: 20230357702Abstract: The present invention relates to a computer implemented method for controlling a fluid coupling network (170), the fluid coupling network (170) being configured to be fluidly couplable to bioreactor (110), a gas source (120), a buffer source (140), a waste port (150), a processing system (160) and a conduit reservoir (208), the fluid coupling network (170) comprising a barrier unit (220) configured to sterilely separate fluid paths within the fluid coupling network (170), the fluid coupling network (170) being controllable to sample the bioreactor (110), the method comprising obtaining (510) a fluid sample from the bioreactor (110), wherein the step of obtaining the fluid sample comprises controlling a flow of fluid from the bioreactor (110) via the conduit reservoir (208) and the fluid coupling network (170) to the waste port (150), to fill the conduit reservoir (208) with the fluid from the bioreactor (110), providing (530) the fluid sample, wherein the step of providing the fluid sample comprises to providType: ApplicationFiled: October 13, 2021Publication date: November 9, 2023Inventors: Tuomo Frigård, Camilla Estmer Nilsson, Patricia Roch, Björn Markus Olovsson, Magnus Wetterhall, Nils Stafström, Inger Salomonsson
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Patent number: 11525111Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.Type: GrantFiled: September 27, 2019Date of Patent: December 13, 2022Assignee: Cytiva Sweden ABInventors: Klaus Gebauer, Andreas Castan, Camilla Estmer-Nilsson, Thomas Falkman, Anders Wilen, Michael Miller, Ralph Stankowski
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Publication number: 20200087605Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.Type: ApplicationFiled: September 27, 2019Publication date: March 19, 2020Applicant: GE Healthcare Bio-Sciences ABInventors: Klaus Gebauer, Andreas Castan, Camilla Estmer-Nilsson, Thomas Falkman, Anders Wilen, Michael Miller, Ralph Stankowski
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Patent number: 10450540Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.Type: GrantFiled: April 27, 2015Date of Patent: October 22, 2019Assignee: GE Healthcare Bio-Sciences ABInventors: Klaus Gebauer, Andreas Castan, Camilla Estmer-Nilsson, Thomas Falkman, Anders Wilen, Michael Miller, Ralph Stankowski
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Patent number: 9983203Abstract: Provided are methods for protein analysis in which proteins to be analyzed are displayed on a population of discrete and dispersible structures, such as beads or other particles, for subsequent affinity reactions and analysis/detection. The proteins to be analyzed may be provided as denatured protein samples in the presence of a denaturing agent.Type: GrantFiled: February 12, 2015Date of Patent: May 29, 2018Assignee: GE HEALTHCARE BIO-SCIENCES ABInventors: Daniel Ivansson, Camilla Estmer Nilsson, Therese Graner, Johan Ohman
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Publication number: 20170044477Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.Type: ApplicationFiled: April 27, 2015Publication date: February 16, 2017Inventors: Klaus Gebauer, Andreas Castan, Camilla Estmer-Nilsson, Thomas Falkman, Anders Wilen, Michael Miller, Ralph Stankowski
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Publication number: 20160320377Abstract: The present invention relates to a method for protein analysis in which the proteins to be analyzed are displayed on a population of discrete and dispersible structures, such as beads or other particles, for subsequent affinity reactions and analysis/detection.Type: ApplicationFiled: February 12, 2015Publication date: November 3, 2016Inventors: Daniel Ivannson, Camilla Estmer-Nilsson, Therese Graner, Johan Ohman
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Patent number: 9340828Abstract: The present invention relates to a simple and efficient method to isolate and purify nucleic acids, preferably genomic DNA, from complex samples compared with available methods, by using a ligand which relies on hydrogen bonding to purify the nucleic acids. Preferably the ligand is bound to magnetic beads/particles. More closely the method comprises adding a sample comprising nucleic acid to a polymer having neutral charge; reversibly binding said nucleic acid to said polymer by hydrogen bonding under pH conditions <5; washing said polymer; and eluting said nucleic acid from said polymer under conditions of pH >5. The method is very suitable for sample preparation of nucleic acids, for example for PCR applications.Type: GrantFiled: October 25, 2012Date of Patent: May 17, 2016Assignee: GE HEALTHCARE BIO-SCIENCES ABInventors: Camilla Estmer Nilsson, Johan Ohman
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Patent number: 9115181Abstract: The present invention relates to a process of enriching one target compound from a liquid, which process comprises at least one step of isolation performed by differentially partitioning between two aqueous phases. In the present invention the phases are formed by adding a thermally responsive, self-associating (i.e. clouding) hydrophilic polymer, and if needed some additional salts, to an aqueous biotechnical solution (such as a fermentation sample or bioseparation process stream) under thermal and other conditions where the solution separates into a one polymer, two-phase system with one phase enriched in the polymer. The target compound is to be found in the phase not enriched in the polymer, while a significant though varying percentage of contaminants may differentially partition to the phase interface or the polymer enriched phase.Type: GrantFiled: January 7, 2010Date of Patent: August 25, 2015Assignee: GE Healthcare Bio-Sciences ABInventors: James Van Alstine, Jamil Shanagar, Rolf Hjorth, Martin Hall, Camilla Estmer Nilsson
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Publication number: 20140272999Abstract: The present invention relates to a simple and efficient method to isolate and purify nucleic acids, preferably genomic DNA, from complex samples compared with available methods, by using a ligand which relies on hydrogen bonding to purify the nucleic acids. Preferably the ligand is bound to magnetic beads/particles. More closely the method comprises adding a sample comprising nucleic acid to a polymer having neutral charge; reversibly binding said nucleic acid to said polymer by hydrogen bonding under pH conditions <5; washing said polymer; and eluting said nucleic acid from said polymer under conditions of pH >5. The method is very suitable for sample preparation of nucleic acids, for example for PCR applications.Type: ApplicationFiled: October 25, 2012Publication date: September 18, 2014Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Camilla Estmer Nilsson, Johan Ohman
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Patent number: 8481298Abstract: The present invention relates to a method of preparing a separation matrix comprising at least one insoluble carrier to which sulphate ligands have been attached via extenders, which method comprises coupling, in a first step, of the sulphate ligands to the extenders and, in a subsequent step, attaching the extenders to an insoluble carrier. The invention also relates to a separation matrix comprised of at least one insoluble carrier to which sulphate ligands have been attached via extenders. Advantageously, no sulphate ligands are directly attached to the insoluble carrier. The extenders may be natural polymers, such as dextran, and the insoluble carrier may be made from natural polymers, such as agarose, or synthetic polymers. The invention also relates to a method of purifying virus, such as influenza virus, using a separation matrix according to the invention.Type: GrantFiled: September 26, 2007Date of Patent: July 9, 2013Assignee: GE Healthcare Bio-Sciences ABInventors: Inger Andersson, Andreas Axen, Peder Bergvall, John Clachan, Camilla Estmer-Nilsson, Lena M. Sandberg
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Publication number: 20120010390Abstract: The present invention relates to a process of enriching one target compound from a liquid, which process comprises at least one step of isolation performed by differentially partitioning between two aqueous phases. In the present invention the phases are formed by adding a thermally responsive, self-associating (i.e. clouding) hydrophilic polymer, and if needed some additional salts, to an aqueous biotechnical solution (such as a fermentation sample or bioseparation process stream) under thermal and other conditions where the solution separates into a one polymer, two-phase system with one phase enriched in the polymer. The target compound is to be found in the phase not enriched in the polymer, while a significant though varying percentage of contaminants may differentially partition to the phase interface or the polymer enriched phase.Type: ApplicationFiled: January 7, 2010Publication date: January 12, 2012Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: James Van Alstine, Jamil Shanagar, Rolf Hjorth, Martin Hall, Camilla Estmer Nilsson
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Publication number: 20100099163Abstract: The present invention relates to a method of preparing a separation matrix comprising at least one insoluble carrier to which sulphate ligands have been attached via extenders, which method comprises coupling, in a first step, of the sulphate ligands to the extenders and, in a subsequent step, attaching the extenders to an insoluble carrier. The invention also relates to a separation matrix comprised of at least one insoluble carrier to which sulphate ligands have been attached via extenders. Advantageously, no sulphate ligands are directly attached to the insoluble carrier. The extenders may be natural polymers, such as dextran, and the insoluble carrier may be made from natural polymers, such as agarose, or synthetic polymers. The invention also relates to a method of purifying virus, such as influenza virus, using a separation matrix according to the invention.Type: ApplicationFiled: September 26, 2007Publication date: April 22, 2010Inventors: Inger Andersson, Andreas Axen, Peder Bergvall, John Clachan, Camilla Estmer-Nilsson, Lena M. Sandberg