Abstract: Apparatus and method for separating whole cells from a mixture, e.g., including liquid, other cell types, nucleic acid material, or other components. Focused acoustic energy may be used to move whole cells in a chamber so that the cells exit the chamber via a first outlet rather than a second outlet. A filter may, or need not, be used to assist in separation.

Abstract: Methods and systems for preparing crystalline particle compositions using focused acoustic processing to control a number or count of crystalline particles generated. Peak incident power of focused acoustic energy used to cause primary nucleation kinetics of a solute may be adjusted to adjust the number or count of crystalline particles.

Abstract: Apparatus and method for separating whole cells from a mixture, e.g., including liquid, other cell types, nucleic acid material, or other components. Focused acoustic energy may be used to move whole cells in a chamber so that the cells exit the chamber via a first outlet rather than a second outlet. A filter may, or need not, be used to assist in separation.

Abstract: The present disclosure relates to a vessel configured to apply pressure greater than ambient pressure and/or provide a reduced headspace to a sample, while subject to focused acoustic treatment. Such treatment may generally result in enhanced effects of sterilization and/or other processing than if the sample is otherwise treated under ambient conditions. The sample may be a medicament that is sterilized under pressure using focused acoustic energy, where the sterilized sample is subsequently delivered to a patient. A fluid delivery cartridge may include a vessel having a transfer end opposite a plunger end. The transfer end may include a sealed cover. A plunger may be movably disposed in the vessel to accommodate entry of a liquid via piercing of the sealed cover. Upon processing (e.g., sterilizing) of the sample with focused acoustic energy, the sample may be appropriately dispensed through the pierced sealed cover.

Abstract: Methods and systems for preparing crystalline particle compositions using focused acoustic processing to control a number or count of crystalline particles generated. Peak incident power of focused acoustic energy used to cause primary nucleation kinetics of a solute may be adjusted to adjust the number or count of crystalline particles.

Abstract: Methods and systems for acoustically treating material using a continuous process in which material may be caused to flow in a continuous or intermittent fashion into/out of an acoustic treatment chamber where the material is exposed to focused acoustic energy. The methods and systems may be arranged to permit continuous processing for extended periods while an acoustic energy source operates at a relatively high power output. Treatment chambers may include features such as an acoustic window and/or a chamber wall which may comprise an acoustically reflective material or a gas/wall interface that serves to reflect acoustic energy to form one or more secondary focal zones. Treatment system configurations relating to arrangements of a treatment chamber relative to an acoustic source and coupling medium, material flow paths, and others are provided.

Abstract: Focused ultrasonic acoustic processing is used to prepare formulations particles ranging between approximately 10 nm and approximately 50 microns (e.g., between 1 micron and 20 microns), or between approximately 10 nm approximately 400 nm (e.g., between 10 nm and 100 nm). Formulations (e.g., nanoformulations) may include a suspension (e.g., nanosuspension), an emulsion (e.g., nanoemulsion) or another small particle system. Formulations may be used as delivery systems for therapeutic agents, e.g., a formulation may include a bioactive agent and a carrier compound such as a surfactant that encapsulates the bioactive agent.

Abstract: The present disclosure relates to a composition that includes an apolipoprotein and a lipid bilayer, and methods and systems for preparing the composition. The apolipoprotein may be incorporated within at least a portion of the lipid bilayer. The lipid bilayer may form a liposome or other suitable carrier for transporting the apolipoprotein. The apolipoprotein incorporated lipid bilayer may provide a suitable delivery vehicle for the apolipoprotein to the body. Compositions of the present disclosure may be formed by exposing a mixture of an apolipoprotein and a lipid formulation to focused acoustic energy which, in some embodiments, may result in a liposome that at least partially encapsulates the apolipoprotein. In some embodiments, apolipoprotein A-V may be incorporated within a liposome, where the apolipoprotein A-V is suitably bioactive, or therapeutic, when delivered to cells and/or into the body of a patient.

Abstract: Methods and systems for acoustically treating material using a continuous process in which material may be caused to flow in a continuous or intermittent fashion into/out of an acoustic treatment chamber where the material is exposed to focused acoustic energy. The methods and systems may be arranged to permit continuous processing for extended periods while an acoustic energy source operates at a relatively high power output. Treatment chambers may include features such as an acoustic window and/or a chamber wall which may comprise an acoustically reflective material or a gas/wall interface that serves to reflect acoustic energy to form one or more secondary focal zones. Treatment system configurations relating to arrangements of a treatment chamber relative to an acoustic source and coupling medium, material flow paths, and others are provided.

Abstract: Methods and systems for acoustically treating material using a continuous process in which material may be caused to flow in a continuous or intermittent fashion into/out of an acoustic treatment chamber where the material is exposed to focused acoustic energy. The methods and systems may be arranged to permit continuous processing for extended periods while an acoustic energy source operates at a relatively high power output. Treatment chambers may include features such as an acoustic window, a heat exchanger, inlet/outlet flow arrangements, an inspection window, insert elements that define a treatment volume size or shape, etc. Treatment system configurations relating to arrangements of a treatment chamber relative to an acoustic source and coupling medium, material flow paths, and others are provided.

Abstract: Methods and systems for acoustically treating material using a continuous process in which material may be caused to flow in a continuous or intermittent fashion into/out of an acoustic treatment chamber where the material is exposed to focused acoustic energy. The methods and systems may be arranged to permit continuous processing for extended periods while an acoustic energy source operates at a relatively high power output. Treatment chambers may include features such as an acoustic window and/or a chamber wall which may comprise an acoustically reflective material or a gas/wall interface that serves to reflect acoustic energy to form one or more secondary focal zones. Treatment system configurations relating to arrangements of a treatment chamber relative to an acoustic source and coupling medium, material flow paths, and others are provided.

Abstract: The present disclosure relates to a vessel configured to apply pressure greater than ambient pressure and/or provide a reduced headspace to a sample, while subject to focused acoustic treatment. Such treatment may generally result in enhanced effects of sterilization and/or other processing than if the sample is otherwise treated under ambient conditions. The sample may be a medicament that is sterilized under pressure using focused acoustic energy, where the sterilized sample is subsequently delivered to a patient. A fluid delivery cartridge may include a vessel having a transfer end opposite a plunger end. The transfer end may include a sealed cover. A plunger may be movably disposed in the vessel to accommodate entry of a liquid via piercing of the sealed cover. Upon processing (e.g., sterilizing) of the sample with focused acoustic energy, the sample may be appropriately dispensed through the pierced sealed cover.

Abstract: Methods and systems for acoustically treating material using a continuous process in which material may be caused to flow in a continuous or intermittent fashion into/out of an acoustic treatment chamber where the material is exposed to focused acoustic energy. The methods and systems may be arranged to permit continuous processing for extended periods while an acoustic energy source operates at a relatively high power output. Treatment chambers may include features such as an acoustic window, a heat exchanger, inlet/outlet flow arrangements, an inspection window, insert elements that define a treatment volume size or shape, etc. Treatment system configurations relating to arrangements of a treatment chamber relative to an acoustic source and coupling medium, material flow paths, and others are provided.

Abstract: Methods and systems for acoustically treating material using a continuous process in which material may be caused to flow in a continuous or intermittent fashion into/out of an acoustic treatment chamber where the material is exposed to focused acoustic energy. The methods and systems may be arranged to permit continuous processing for extended periods while an acoustic energy source operates at a relatively high power output. Treatment chambers may include features such as an acoustic window, a heat exchanger, inlet/outlet flow arrangements, an inspection window, insert elements that define a treatment volume size or shape, etc. Treatment system configurations relating to arrangements of a treatment chamber relative to an acoustic source and coupling medium, material flow paths, and others are provided.

Abstract: Methods and systems for preparing nanocrystalline compositions using focused acoustic processing to cause and/or enhance crystal growth. A flow through system may be employed to expose sample material having a volume of greater or less than 30 mL to focused acoustic energy while flowing through a process chamber at a rate of at least 0.1 mL/min. Sample material may be processed by a suitable focused acoustic field in a cyclic fashion and/or with adjustment of processing parameters based on monitored characteristics of the sample, such as level of crystallinity. Nanocrystalline particles within the sample may have a tight particle size distribution with an average particle size between 10 nm and 1 micron. Stable nanocrystalline compositions may be reproducibly prepared using focused acoustics to have controllable morphologies and dimensions.

Abstract: Methods and systems for acoustically treating material using a continuous process in which material may be caused to flow in a continuous or intermittent fashion into/out of an acoustic treatment chamber where the material is exposed to focused acoustic energy. The methods and systems may be arranged to permit continuous processing for extended periods while an acoustic energy source operates at a relatively high power output. Treatment chambers may include features such as an acoustic window and/or a chamber wall which may comprise an acoustically reflective material or a gas/wall interface that serves to reflect acoustic energy to form one or more secondary focal zones. Treatment system configurations relating to arrangements of a treatment chamber relative to an acoustic source and coupling medium, material flow paths, and others are provided.

Abstract: Methods and systems for acoustically treating material using a continuous process in which material may be caused to flow in a continuous or intermittent fashion into/out of an acoustic treatment chamber where the material is exposed to focused acoustic energy. The methods and systems may be arranged to permit continuous processing for extended periods while an acoustic energy source operates at a relatively high power output. Treatment chambers may include features such as an acoustic window, a heat exchanger, inlet/outlet flow arrangements, an inspection window, insert elements that define a treatment volume size or shape, etc. Treatment system configurations relating to arrangements of a treatment chamber relative to an acoustic source and coupling medium, material flow paths, and others are provided.