Patents by Inventor Casey Stankewicz

Casey Stankewicz has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • MACS-BASED PURIFICATION OF STEM CELL-DERIVED RETINAL PIGMENT EPITHELIUM
    Publication number: 20220033770
    Abstract: Provided herein are methods of enriching a retinal pigment epithelium (RPE) cell population derived from stem cells. Such a method may comprise removing contaminating cells through the depletion of CD24 positive cells, CD56 positive cells, and/or CD90 positive cells from a starting population of RPE cells.
    Type: Application
    Filed: October 4, 2021
    Publication date: February 3, 2022
    Applicant: FUJIFILM Cellular Dynamics, Inc.
    Inventors: Nathan MEYER, Lucas CHASE, Casey STANKEWICZ
  • MACS-based purification of stem cell-derived retinal pigment epithelium
    Patent number: 11162070
    Abstract: Provided herein are methods of enriching a retinal pigment epithelium (RPE) cell population derived from stem cells. Such a method may comprise removing contaminating cells through the depletion of CD24 positive cells, CD56 positive cells, and/or CD90 positive cells from a starting population of RPE cells.
    Type: Grant
    Filed: September 7, 2016
    Date of Patent: November 2, 2021
    Assignee: FUJIFILM Cellular Dynamics, Inc.
    Inventors: Nathan Meyer, Lucas Chase, Casey Stankewicz
  • Cardiomyocyte production
    Patent number: 10604739
    Abstract: Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
    Type: Grant
    Filed: April 27, 2017
    Date of Patent: March 31, 2020
    Assignee: FUJIFILM Cellular Dynamics, Inc.
    Inventors: Casey Stankewicz, Matt Riley, Nathaniel Beardsley, Wen Bo Wang, Peter Fuhrken, Steven Kattman
  • Cardiomyocyte production
    Patent number: 9957482
    Abstract: Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
    Type: Grant
    Filed: March 23, 2015
    Date of Patent: May 1, 2018
    Assignee: Cellular Dynamics International, Inc.
    Inventors: Casey Stankewicz, Matt Riley, Nathaniel Beardsley, Wen Bo Wang, Peter Fuhrken, Steven Kattman
  • CARDIOMYOCYTE PRODUCTION
    Publication number: 20170226481
    Abstract: Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
    Type: Application
    Filed: April 27, 2017
    Publication date: August 10, 2017
    Applicant: Cellular Dynamics International, Inc.
    Inventors: Casey STANKEWICZ, Matt RILEY, Nathaniel BEARDSLEY, Wen Bo WANG, Peter FUHRKEN, Steven KATTMAN
  • MACS-BASED PURIFICATION OF STEM CELL-DERIVED RETINAL PIGMENT EPITHELIUM
    Publication number: 20170067017
    Abstract: Provided herein are methods of enriching a retinal pigment epithelium (RPE) cell population derived from stem cells. Such a method may comprise removing contaminating cells through the depletion of CD24 positive cells, CD56 positive cells, and/or CD90 positive cells from a starting population of RPE cells.
    Type: Application
    Filed: September 7, 2016
    Publication date: March 9, 2017
    Applicant: Cellular Dynamics International, Inc.
    Inventors: Nathan MEYER, Lucas CHASE, Casey STANKEWICZ
  • Priming of pluripotent stem cells for neural differentiation
    Patent number: 9487752
    Abstract: Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including priming stem cells for neural differentiation in a culture medium essentially free of growth factors such as FGF and TGF?. As an advantage, the neural cells may be provided with improved consistency and purity.
    Type: Grant
    Filed: March 30, 2012
    Date of Patent: November 8, 2016
    Assignee: Cellular Dynamics International, Inc.
    Inventors: Nathan Meyer, Matthew George, Casey Stankewicz, Deepika Rajesh
  • Cardiomyocyte production
    Patent number: 9365827
    Abstract: Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: June 14, 2016
    Assignee: Cellular Dynamics International, Inc.
    Inventors: Casey Stankewicz, Matt Riley, Nathaniel Beardsley, Wen Bo Wang, Peter Fuhrken, Steven Kattman
  • Titration of differentiation medium components
    Patent number: 9181529
    Abstract: Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including screening of optimal differentiation conditions for a selected stem cell clone in a selected batch of culture medium and use of the determined optimal condition for differentiation into a specific cell lineage.
    Type: Grant
    Filed: October 19, 2011
    Date of Patent: November 10, 2015
    Assignee: Cellular Dynamics International, Inc.
    Inventors: Steven Kattman, Wen Bo Wang, Peter Fuhrken, Nathan Meyer, Matthew George, Casey Stankewicz
  • CARDIOMYOCYTE PRODUCTION
    Publication number: 20150191697
    Abstract: Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
    Type: Application
    Filed: March 23, 2015
    Publication date: July 9, 2015
    Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.
    Inventors: Casey STANKEWICZ, Matt RILEY, Nathaniel BEARDSLEY, Wen Bo WANG, Peter FUHRKEN, Steven KATTMAN
  • Cardiomyocyte production
    Patent number: 8415155
    Abstract: Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
    Type: Grant
    Filed: October 19, 2010
    Date of Patent: April 9, 2013
    Assignee: Cellular Dynamics International, Inc.
    Inventors: Casey Stankewicz, Matt Riley, Nathaniel Beardsley, Wen Bo Wang, Peter Fuhrken, Steven Kattman
  • PRIMING OF PLURIPOTENT STEM CELLS FOR NEURAL DIFFERENTIATION
    Publication number: 20120276063
    Abstract: Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including priming stem cells for neural differentiation in a culture medium essentially free of growth factors such as FGF and TGF?. As an advantage, the neural cells may be provided with improved consistency and purity.
    Type: Application
    Filed: March 30, 2012
    Publication date: November 1, 2012
    Inventors: Nathan Meyer, Matthew George, Casey Stankewicz, Deepika Rajesh
  • TITRATION OF DIFFERENTIATION MEDIUM COMPONENTS
    Publication number: 20120129211
    Abstract: Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including screening of optimal differentiation conditions for a selected stem cell clone in a selected batch of culture medium and use of the determined optimal condition for differentiation into a specific cell lineage.
    Type: Application
    Filed: October 19, 2011
    Publication date: May 24, 2012
    Inventors: STEVEN KATTMAN, Wen Bo Wang, Peter Fuhrken, Nathan Meyer, Matthew George, Casey Stankewicz
  • CARDIOMYOCYTE PRODUCTION
    Publication number: 20110097799
    Abstract: Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.
    Type: Application
    Filed: October 19, 2010
    Publication date: April 28, 2011
    Inventors: Casey Stankewicz, Matt Riley, Nathaniel Beardsley, Wen Bo Wang, Peter Fuhrken, Steven Kattman
  • Metal ion mediated fluorescence superquenching assays, kits and reagents
    Publication number: 20070238143
    Abstract: Reagents and assays for kinase, phosphatase and protease enzyme activity which employ metal ion-phosphate ligand specific binding and fluorescent polymer superquenching are described. The assays provide a general platform for the measurement of kinase, phosphatase and protease enzyme activity using peptide and protein substrates. Reagents and assays based on DNA hybridization and reagents and assays for proteins which employ aptamers, antibodies and other ligands are also described.
    Type: Application
    Filed: September 7, 2005
    Publication date: October 11, 2007
    Inventors: Wensheng Xia, Frauke Rininsland, Sriram Kumaraswamy, Stuart Kushon, Liangde Lu, Xiaobo Shi, Casey Stankewicz, Shannon Wittenburg, Komandoor Achyuthan, Duncan McBranch, David Whitten
  • Metal mediated fluorescence superquenching assays for detection of phosphorylation and dephosphorylation of quencher-labeled substrates
    Publication number: 20060292653
    Abstract: A method includes contacting an analyte; a phosphorylatable or dephosphorylatable substrate bound to a quencher; and a sensor that includes at least one fluorescent polymer complexed to one or more metal species; to form a composition; and determining the fluorescence of the composition, if any; wherein when the substrate is phosphorylated, an association occurs between the substrate and the sensor such that fluorescent superquenching occurs between the fluorescent polymer and the quencher.
    Type: Application
    Filed: March 20, 2006
    Publication date: December 28, 2006
    Inventors: Frauke Rininsland, Casey Stankewicz