Abstract: A method of determining a normalized quantity of an analyte adhering to beads in a detection area of a bead-based assaying system, the method comprising: a) causing a complex of the analyte to fluorescently or chemically emit a first light, or to release a dye; b) measuring an integrated intensity of the first light emitted from the beads in the detection area, or a concentration of the dye released from the beads in the detection area, or both; c) causing light to interact with the beads in the detection area, the interaction not depending on whether or how much analyte is adhering to the beads; d) measuring a second light resulting from the interaction with the beads which does not depend on the analyte; and e) determining the normalized quantity of analyte from the integrated intensity of the first light or concentration of the dye or both, and from the measured second light.

Abstract: A method of determining a normalized quantity of an analyte adhering to beads in a detection area of a bead-based assaying system, the method comprising: a) causing a complex of the analyte to fluorescently or chemically emit a first light, or to release a dye; b) measuring an integrated intensity of the first light emitted from the beads in the detection area, or a concentration of the dye released from the beads in the detection area, or both; c) causing light to interact with the beads in the detection area, the interaction not depending on whether or how much analyte is adhering to the beads; d) measuring a second light resulting from the interaction with the beads which does not depend on the analyte; and e) determining the normalized quantity of analyte from the integrated intensity of the first light or concentration of the dye or both, and from the measured second light.

Abstract: A method of and device for sampling polycyclic aromatic hydrocarbons (PAHs) in the ambient environment, the method including: (a) providing a polymer film for collecting the PAHs; (b) exposing the polymer film to a gas containing the PAHs, and (c) performing an analysis of the PAHs collected by the polymer film, the analysis being selected from the group consisting of fluorescence analysis and Fourier Transform Imaging Microscopy (FT-SIM).

Abstract: A method of and device for sampling polycyclic aromatic hydrocarbons (PAHs) in the ambient environment, the method including: (a) providing a polymer film for collecting the PAHs; (b) exposing the polymer film to a gas containing the PAHs, and (c) performing an analysis of the PAHs collected by the polymer film, the analysis being selected from the group consisting of fluorescence analysis and Fourier Transform Imaging Microscopy (F-SIM).