Abstract: Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86th site, the 373rd site, the 374th site, the 496th site, the 507th site, or the 747th site of agarase with an amino acid sequence as shown in SEQ ID NO. 1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.

Abstract: Layered super-hydrophilic Ti—Cu-MOFs and a preparation method and use thereof provided, which relates to the technical field of composite materials. According to the present application, copper ions and titanium ions are used as bimetallic centers to have a coordination reaction with rigid aromatic amine ligands, and layered super-hydrophilic Ti—Cu-MOFs with a lamellar structure is efficiently synthesized. Compared with the prior technology, the preparation method of the present application is simple, and the morphology and structure are controllable; the surface of the prepared layered super-hydrophilic Ti—Cu-MOFs are rich in hydroxyl groups, and have high adsorption capacity for phospholipid co-extracts in clinical biological samples, and thus can be used for the analysis of phenothiazine drugs and metabolites thereof in biological samples and realize one-step separation and purification, thus greatly improving the pretreatment efficiency of clinical biological samples and shortening the analysis time.

Abstract: Disclosed herein are gene therapy vectors used for efficiently transducing cells to express a human ?-globin gene. Specifically disclosed is an expression vector which comprises: an expression cassette for a ?-globin gene, which comprises exons and introns of human ?-globin gene, as well as cis-acting elements including one or more of WPRE, SV40 polyadenylation signal and/or SV40 ori. The disclosed expression vectors have significantly enhanced viral vector packaging efficiency in viral vector packaging cell lines, which leads to effective integration of lentiviral vectors and high expression level of ?-globin gene in target cells. Also disclosed are pharmaceutical compositions and therapeutic methods utilizing such expression vectors.

Abstract: The application discloses an optical cable assembly and an optical cable testing method. The optical cable assembly comprises: an optical cable wire with a test end; a spool for winding the optical cable; a connection assembly has an optical cable connection port and a test port, the connection assembly is configured to connect objects at both ends of the connection assembly, and the test end of the optical cable is connected to the connection assembly as an object at one end of the optical cable connection port, the test port is located at an operable area of the spool, configured to connect the test equipment to form an optical path between the test equipment and the optical cable wire when the optical cable is tested.

Abstract: The present disclosure provides a loudspeaker assembly and an electronic device, relating to the technical field of electronic devices, so as to solve the problems of single sound effect and lack of good sound effect. The loudspeaker assembly includes a first housing, a second housing, a loudspeaker vibrator and an adjusting mechanism. The first housing and the loudspeaker vibrator form a first cavity, and the second housing and the loudspeaker vibrator form a second cavity. The adjusting mechanism includes a driving mechanism and a filling part. The driving mechanism drives the filling part to move, so as to change the volume of the filling part extending into the second cavity.

Abstract: An optical camera lens includes a first camera lens component including at least one first lens and a second camera lens component including a second lens barrel and at least one second lens mounted in the second lens barrel. The first and second lenses together constitute an imageable optical system. At least one of the two camera lens components has a smooth region located on an end surface of the camera lens component, and a connecting medium for fixing the first and second camera lens components together. A corresponding assembly method of the optical camera lens and a corresponding camera module and an assembly method thereof are also provided. The optical camera lens can improve a pre-positioning accuracy by improving a height measurement accuracy, and can use a distance measuring point as a feature point of image recognition so as to perform pre-positioning and active calibration.

Abstract: An outdoor tent with a modular base includes a main body and a base, the main body being detachably arranged on the base. The base includes a first module and several splicing modules, said several splicing modules being detachably connected to the periphery of the first module, and the first module can be folded to form a box structure, and said several splicing modules can be independently folded and stored in the box formed by the first module. When the base is in the storage state, the first module is folded to form a box structure in which items can be placed, and the second module, third module, fourth module and fifth module are folded and accommodated in the box formed by the first module, reducing space occupation during transportation, improving utilization rate of user space during transportation, and making it easy to carry and transport.

Abstract: Disclosed are compounds of Formula I, methods of using the compounds for inhibiting KRAS activity and pharmaceutical compositions comprising such compounds. The compounds are useful in treating, preventing or ameliorating diseases or disorders associated with KRAS activity such as cancer.

Abstract: There is provided a display substrate, including: a base; light-emitting units on a side of the base; a flat light-shielding functional layer, including a black matrix and a first planarization layer, on a side of the light-emitting units away from the base, light outgoing openings being provided in the black matrix and being in one-to-one correspondence with the light-emitting units, and the first planarization layer at least filling the light outgoing openings; and a color filter layer, including color filter patterns in one-to-one correspondence with the light outgoing openings, on a side of the flat light-shielding functional layer away from the base, an orthographic projection of each color filter pattern on the base covering an orthographic projection of the light outgoing opening corresponding to the color filter pattern on the base. A method for manufacturing a display substrate, a display panel and a display apparatus are further provided.

Abstract: Performing UCI multiplexing includes generating PUCCH resources Z at an L1 HP procedure and an L1 LP procedure. The L1 HP procedure and the L1 LP procedure results in a plurality of HP PUCCHs and LP PUCCHs, respectively. A modified procedure for Set Q to resources Z is performed with the plurality of HP PUCCHs and LP PUCCHs as inputs. The plurality of HP PUCCHs according to starting symbols is scanned to identify an earliest starting HP PUCCH. A PUCCH from the plurality of LP PUCCHs or HP PUCCHS that overlaps with the earliest starting HP PUCCH is collected. Inter-L1 priority multiplexing is performed on the earliest starting HP PUCCH and the collected PUCCH, which may result in a new HP PUCCH. The new HP PUCCH is inserted into the Set Q. The modified procedure is repeated until there is no overlap among the HP PUCCHs and the LP PUCCHs.

Abstract: A tent with a charging device and wiring thereof includes a main body that includes a frame and a wall, the frame being connected to the wall. The tent also includes an electrical component, which includes an electrical appliance, a power supply and an electric wire. The electrical appliance and the power supply are electrically connected through the electric wire, and the electrical appliance and the electric wire are detachably or fixedly arranged on the frame and/or the wall. For the tent with a charging device and wiring thereof, electrical appliances and electric wires are reasonably arranged on the frame or the wall of the tent in a fixed or manner, by which layout of electrical appliances and electric wires is more reasonable and regular, and the electrical appliances and electric wires will not occupy activity space in the tent, effectively improving users' experience on tents.

Abstract: A floor brush, a cleaning device and a roller brush disassembly method; wherein the floor brush includes: a floor brush body, one end of the floor brush body being provided with a driving means; a roller brush, and one end of the roller brush is drivable-connected to the driving means; a driving shaft of the driving means is capable of rotating in a first direction of rotation, causing the roller brush to move relatively to the driving shaft and be off the preset mounting position; the first direction of rotation is opposite to the direction of rotation of the roller brush when performing cleaning. Roller brush is driven to move by means of the driving means, and can be quickly disassembled from the floor brush body.

Abstract: A method for assembling an optical lens includes preparing a first lenses part including a first lens and a second lenses part including a second lens, capturing the first lenses part, and adjusting a posture of the first lenses part according to a distance measurement result, so that an included angle between a first end surface of the first lenses part and a second end surface of the second lenses part is less than a threshold of inclination angle. The method also includes adjusting the first lenses part at degree of freedom of linear movement to complete pre-positioning, performing an active alignment of a position of the first lenses part at the degree of freedom of linear movement according to an actual imaging result, and fixing the first and second lenses parts together, so that their relative positions are maintained at the relative positions determined by the active alignment.

Abstract: Provided are a tray and a cleaning apparatus system. The tray includes: a tray body, where a roller brush accommodation region is arranged on an outer surface of the tray body, and a mounting cavity is provided in the tray body; and a drying unit, where the drying unit is mounted in the mounting cavity, and the drying unit runs to perform drying treatment on the roller brush accommodation region and an object in the roller brush accommodation region. The tray may be used with a cleaning machine. When the cleaning machine is disposed on the tray, a roller brush on the cleaning machine is located in the roller brush accommodation region, and the drying unit runs to perform drying treatment on the roller brush accommodation region and the object in the roller brush accommodation region.

Abstract: A touch-controlling region of a touch-controlling base plate includes a touch-controlling layer; a non-touch-controlling region includes a plurality of lead wires and at least one touch-controlling unit; the touch-controlling layer includes a plurality of first electrically conductive units and a plurality of second electrically conductive units; the touch-controlling unit is located on at least a first side of the touch-controlling layer; the lead wires are connected to the touch-controlling unit, and are located on a same one side of the touch-controlling layer; and a plurality of lines of the first electrically conductive units that are arranged in the first channel direction are grouped into two groups, and at least one of the groups is connected to the lead wires via each line of the first electrically conductive units that are located at an edge of the first side of the touch-controlling layer.

Abstract: Disclosed are a self-righting unmanned ship suitable for adverse sea conditions and a self-righting working mode thereof, belonging to the field of unmanned ship equipment and techniques. The unmanned ship comprises a main hull, a self-righting deck, an equipment and pipeline mast, a propeller, a radar, an air inlet and exhaust system, and a main engine system. Through the design of a watertight deck, the hull of the unmanned ship has a self-righting function, avoiding the possibility of the unmanned ship itself turning over, without installing additional self-righting equipment. Meanwhile, the internal structure and the self-righting working mode of the unmanned ship make it possible for the hull to automatically turn off the main engine and the air inlet and exhaust system when the heeling angle of the hull exceeds a certain angle, making the whole ship watertight.

Abstract: A method for displaying a smooth movement of a vehicle on a map may include obtaining a route, a last real-time location of the vehicle, and a last uploading time point. The method may also include obtaining driving data of one or more neighboring vehicles and determining a predicted location of the vehicle at a prediction generating time point. The method may further include displaying a smooth movement of the vehicle from the last real-time location to the predicted location on a map. A method for displaying a driving path of a vehicle on a map may include obtaining a request for displaying a driving path of a vehicle, location information of the vehicle, and scene related information associated with the driving path. The method may further include verifying the location information based on the scene related information and displaying the driving path of the vehicle on a map.

Abstract: A method for assembling an optical lens includes preparing a first lenses part including a first lens and a second lenses part including a second lens, capturing the first lenses part, and adjusting a posture of the first lenses part according to a distance measurement result, so that an included angle between a first end surface of the first lenses part and a second end surface of the second lenses part is less than a threshold of inclination angle. The method also includes adjusting the first lenses part at degree of freedom of linear movement to complete pre-positioning, performing an active alignment of a position of the first lenses part at the degree of freedom of linear movement according to an actual imaging result, and fixing the first and second lenses parts together, so that their relative positions are maintained at the relative positions determined by the active alignment.

Abstract: A touch-controlling region of a touch-controlling base plate includes a touch-controlling layer; a non-touch-controlling region includes a plurality of lead wires and at least one touch-controlling unit; the touch-controlling layer includes a plurality of first electrically conductive units and a plurality of second electrically conductive units; the touch-controlling unit is located on at least a first side of the touch-controlling layer; the lead wires are connected to the touch-controlling unit, and are located on a same one side of the touch-controlling layer; and a plurality of lines of the first electrically conductive units that are arranged in the first channel direction are grouped into two groups, and at least one of the groups is connected to the lead wires via each line of the first electrically conductive units that are located at an edge of the first side of the touch-controlling layer.

Abstract: An optical camera lens includes a first camera lens component including at least one first lens and a second camera lens component including a second lens barrel and at least one second lens mounted in the second lens barrel. The first and second lenses together constitute an imageable optical system. At least one of the two camera lens components has a smooth region located on an end surface of the camera lens component, and a connecting medium for fixing the first and second camera lens components together. A corresponding assembly method of the optical camera lens and a corresponding camera module and an assembly method thereof are also provided. The optical camera lens can improve a pre-positioning accuracy by improving a height measurement accuracy, and can use a distance measuring point as a feature point of image recognition so as to perform pre-positioning and active calibration.