Patents by Inventor Charisse M. Buising

Charisse M. Buising has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of regeneration of Medicago sativa and expressing foreign DNA in same
    Patent number: 6566137
    Abstract: The invention relates to improved transformation and regeneration of alfalfa, Medicago sativa. Regeneration and transformation of alfalfa is made possible by use of immature cotyledons of alfalfa. By using immature cotyledon tissue, it is possible to regenerate and transform varieties of alfalfa never before capable of regeneration and transformation.
    Type: Grant
    Filed: May 5, 1999
    Date of Patent: May 20, 2003
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Charisse M. Buising, Dwight Tomes
  • Methods of regeneration of Medicago sativa and expressing foreign DNA in same
    Patent number: 5324646
    Abstract: The invention relates to improved transformation and regeneration of alfalfa, Medicago sativa.A method is used to transform alfalfa by using particle acceleration. Optimum results with mature cotyledons occurs when bombarding after 24 to 120 hours of imbibing water. Regeneration and transformation of alfalfa is greatly improved by using immature cotyledons or embryos of immature cotyledons for transformation and regeneration. Immature cotyledons include those up to about 25 days past pollination, and preferably include cotyledons excised at 10-15 days past pollination, most preferably including those excised at about 10 days past pollination. These cotyledons have a light green to translucent appearance. Plants resulting from bombardment of somatic embryos of immature cotyledons retain regeneration ability.
    Type: Grant
    Filed: January 6, 1992
    Date of Patent: June 28, 1994
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Charisse M. Buising, Dwight Tomes, Janice Schmidt
  • Soybean transformation by microparticle bombardment
    Patent number: 5322783
    Abstract: Plant cells in tissue culture are more efficiently transformed in a method which involves treatment with a cytokinin followed by incubation for a period sufficient to permit undifferentiated cells in cotyledonary node tissue to differentiate into meristematic cells and to permit the cells to enter the phases between the G1 and division phases of development.
    Type: Grant
    Filed: October 17, 1989
    Date of Patent: June 21, 1994
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Dwight Tomes, Dennis Bidney, Charisse M. Buising