Patents by Inventor Charles A. Lissandrello
Charles A. Lissandrello has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240109067Abstract: Miniaturized DNA microarrays are described to be used in conjunction with microfluidic channels or microcentrifuge tubes and microcentrifuge filters to reduce sample size, incubation time and to increase overall binding efficiency.Type: ApplicationFiled: November 28, 2023Publication date: April 4, 2024Inventors: Kirsty A. McFarland, Charles A. Lissandrello, Andrew P. Magyar, Erin Rosenberger
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Publication number: 20240084236Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.Type: ApplicationFiled: November 21, 2023Publication date: March 14, 2024Inventors: Vishal Tandon, Charles A. Lissandrello, Jenna L. Balestrini, Jonathan R. Coppeta, Patricia A. Swierk
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Patent number: 11890620Abstract: Miniaturized DNA microarrays are described to be used in conjunction with microfluidic channels or microcentrifuge tubes and microcentrifuge filters to reduce sample size, incubation time and to increase overall binding efficiency.Type: GrantFiled: October 12, 2018Date of Patent: February 6, 2024Assignee: The Charles Stark Draper Laboratory, Inc.Inventors: Kirsty A. McFarland, Charles A. Lissandrello, Andrew P. Magyar, Erin Rosenberger
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Publication number: 20240002919Abstract: A removable cartridge to be used in a system for extracting and detecting nucleic acids from heterogeneous samples includes a plurality of reservoirs defining at least a first wash buffer reservoir for holding a first wash buffer and a microfluidic assembly configured to attach to the plurality of reservoirs. The microfluidic assembly includes at least one sample reservoir and a nucleic acid extraction matrix in fluid communication to an automated sample preparation (ASP) reservoir through a first flow channel defined by the microfluidic assembly. An assay chamber is in fluid communication with a third flow channel and with the waste reservoir through a fourth flow channel such that a labeled nucleic acid-containing sample flows through the assay chamber and then to the waste reservoir, wherein vibration-driven mixing agitates fluids while present in the assay chamber. Finally, a nucleic acid-detecting microarray module is positioned in the assay chamber.Type: ApplicationFiled: May 1, 2023Publication date: January 4, 2024Applicant: The Charles Stark Draper Laboratory, Inc.Inventors: Charles A. LISSANDRELLO, Aditi R. NAIK, Diana J. LEWIS, Erin ROSENBERGER, Joseph Neil URBAN, Jason FIERING, Caleb R. Bell, Cait Ni Chleirigh, Ernest Kim
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Patent number: 11859162Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.Type: GrantFiled: August 30, 2019Date of Patent: January 2, 2024Assignee: The Charles Stark Draper Laboratory, Inc.Inventors: Vishal Tandon, Charles A. Lissandrello, Jenna L. Balestrini, Jonathan R. Coppeta, Patricia A. Swierk
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Publication number: 20230234058Abstract: A microfluidic system can include a substrate comprising an elastic material and defining a microfluidic channel. The substrate can have a first set of dimensions defining a thickness of a wall of the microfluidic channel and a second set of dimensions defining a width of the microfluidic channel. A transducer can be mechanically coupled with the substrate. The transducer can be operated at a predetermined frequency different from a primary thickness resonant frequency of the transducer. A thickness and a width of the transducer can be selected based on the first set of dimensions defining the thickness of the wall of the microfluidic channel and the second set of dimensions defining the width of the microfluidic channel.Type: ApplicationFiled: April 3, 2023Publication date: July 27, 2023Applicant: The Charles Stark Draper Laboratory, Inc.Inventors: Ryan Dubay, Jason Fiering, Rebecca Christianson, Jason Durant, Charles Lissandrello
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Publication number: 20230183631Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated and continuous process that includes flowing the cells between electroporation electrodes to facilitate delivery of a payload into the cells, while acoustophoretically focusing the cells. Also described is a control method for the acoustophoretic focusing of cells that includes detecting locations of cells flowing through a channel, such as with an image analytics system, and modulating a drive signal to an acoustic transducer to change the locations of the cells flowing in the channel. Finally, an electroporation driver module is described that uses a digital to analog converter for generating an electroporation waveform and an amplifier for amplifying the electroporation waveform for application to electroporation electrodes.Type: ApplicationFiled: December 19, 2022Publication date: June 15, 2023Applicant: The Charles Stark Draper Laboratory, Inc.Inventors: Vishal Tandon, Charles A. Lissandrello, Ryan A. Dubay, Rebecca Christianson, Jenna Leigh Balestrini, Peter Hsi, Jason Fiering
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Patent number: 11618022Abstract: A microfluidic system can include a substrate comprising an elastic material and defining a microfluidic channel. The substrate can have a first set of dimensions defining a thickness of a wall of the microfluidic channel and a second set of dimensions defining a width of the microfluidic channel. A transducer can be mechanically coupled with the substrate. The transducer can be operated at a predetermined frequency different from a primary thickness resonant frequency of the transducer. A thickness and a width of the transducer can be selected based on the first set of dimensions defining the thickness of the wall of the microfluidic channel and the second set of dimensions defining the width of the microfluidic channel.Type: GrantFiled: April 3, 2020Date of Patent: April 4, 2023Assignee: The Charles Stark Draper Laboratory, Inc.Inventors: Ryan Dubay, Jason Fiering, Rebecca Christianson, Jason Durant, Charles Lissandrello
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Patent number: 11591561Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated and continuous process that includes flowing the cells between electroporation electrodes to facilitate delivery of a payload into the cells, while acoustophoretically focusing the cells. Also described is a control method for the acoustophoretic focusing of cells that includes detecting locations of cells flowing through a channel, such as with an image analytics system, and modulating a drive signal to an acoustic transducer to change the locations of the cells flowing in the channel. Finally, an electroporation driver module is described that uses a digital to analog converter for generating an electroporation waveform and an amplifier for amplifying the electroporation waveform for application to electroporation electrodes.Type: GrantFiled: October 23, 2018Date of Patent: February 28, 2023Assignee: The Charles Stark Draper Laboratory, Inc.Inventors: Vishal Tandon, Charles A. Lissandrello, Ryan A. Dubay, Rebecca Christianson, Jenna Leigh Balestrini, Peter Hsi, Jason Fiering
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Patent number: 11385400Abstract: The material stack of the present disclosure can be used for fabricating optical waveguides that are thin and flexible, and that can bend light around small turns. The stack of materials can include a polymer core and a cladding, which together can create a large difference in refractive index. As a result, light can remain within the core even when bent around radii where standard glass fibers could fail.Type: GrantFiled: November 13, 2017Date of Patent: July 12, 2022Assignee: The Charles Stark Draper Laboratory, Inc.Inventors: Jesse J. Wheeler, Joseph J. Register, Parshant Kumar, Carlos A. Segura, Charles A. Lissandrello, John J. LeBlanc
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Publication number: 20210290949Abstract: Implantable nerve cuffs and methods for constructing or manufacturing the same are provided. Also provided is a method for installing a nerve into a nerve passage in the nerve cuff and a system using the nerve cuff. The nerve cuff is configured to retain one or more signal carrying elements such as electrodes proximal to a peripheral nerve in a human or animal subject. The nerve cuff may be constructed using a 3D printing method.Type: ApplicationFiled: July 27, 2017Publication date: September 23, 2021Applicants: GALVANI BIOELECTRONICS LIMITED, TRUSTEES OF BOSTON UNIVERSITYInventors: Bradley J. HOLINSKI, Timothy GARDNER, Charles A. LISSANDRELLO, Alice E. WHITE, Winthrop GILLIS, Jun SHEN, Timothy OTCHY, Christos MICHAS
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Publication number: 20210220823Abstract: Miniaturized DNA microarrays are described to be used in conjunction with microfluidic channels or microcentrifuge tubes and microcentrifuge filters to reduce sample size, incubation time and to increase overall binding efficiency.Type: ApplicationFiled: October 12, 2018Publication date: July 22, 2021Inventors: Kirsty A. McFarland, Charles A. Lissandrello, Andrew P. Magyar, Erin Rosenberger
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Publication number: 20200316601Abstract: A microfluidic system can include a substrate comprising an elastic material and defining a microfluidic channel. The substrate can have a first set of dimensions defining a thickness of a wall of the microfluidic channel and a second set of dimensions defining a width of the microfluidic channel. A transducer can be mechanically coupled with the substrate. The transducer can be operated at a predetermined frequency different from a primary thickness resonant frequency of the transducer. A thickness and a width of the transducer can be selected based on the first set of dimensions defining the thickness of the wall of the microfluidic channel and the second set of dimensions defining the width of the microfluidic channel.Type: ApplicationFiled: April 3, 2020Publication date: October 8, 2020Inventors: Ryan Dubay, Jason Fiering, Rebecca Christianson, Jason Durant, Charles Lissandrello
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Publication number: 20200071727Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.Type: ApplicationFiled: August 30, 2019Publication date: March 5, 2020Inventors: Vishal Tandon, Charles A. Lissandrello, Jenna L. Balestrini, Jonathan R. Coppeta, Patricia A. Swierk
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Publication number: 20190292565Abstract: A system for sequential exposure of particles to different fluid streams includes an acoustic actuator device for acoustically driving one or more substrates and a microchannel device of the one or more substrates that receive particles in a first flowing fluid, moves the particles to a second flowing fluid, then moves the particles out of the second flowing fluid using acoustic radiation generated by the acoustic actuator device. The system can control residence times in the streams. According to one use, the first flowing fluid is a cell buffer and the second flowing media is an electroporation buffer. An electroporation system is placed in or downstream of the acoustic actuator device. However, in other uses, the second flowing media might be a wash buffer.Type: ApplicationFiled: March 20, 2019Publication date: September 26, 2019Inventors: Vishal Tandon, Charles A. Lissandrello, Jenna Leigh Balestrini, Ryan A. Dubay
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Publication number: 20190119624Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated and continuous process that includes flowing the cells between electroporation electrodes to facilitate delivery of a payload into the cells, while acoustophoretically focusing the cells. Also described is a control method for the acoustophoretic focusing of cells that includes detecting locations of cells flowing through a channel, such as with an image analytics system, and modulating a drive signal to an acoustic transducer to change the locations of the cells flowing in the channel. Finally, an electroporation driver module is described that uses a digital to analog converter for generating an electroporation waveform and an amplifier for amplifying the electroporation waveform for application to electroporation electrodes.Type: ApplicationFiled: October 23, 2018Publication date: April 25, 2019Inventors: Vishal Tandon, Charles A. Lissandrello, Ryan A. Dubay, Rebecca Christianson, Jenna Leigh Balestrini, Peter Hsi, Jason Fiering
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Publication number: 20180136389Abstract: The material stack of the present disclosure can be used for fabricating optical waveguides that are thin and flexible, and that can bend light around small turns. The stack of materials can include a polymer core and a cladding, which together can create a large difference in refractive index. As a result, light can remain within the core even when bent around radii where standard glass fibers could fail.Type: ApplicationFiled: November 13, 2017Publication date: May 17, 2018Inventors: Jesse J. Wheeler, Joseph J. Register, Parshant Kumar, Carlos A. Segura, Charles A. Lissandrello, John J. LeBlanc
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Publication number: 20180133506Abstract: This disclosure provides a device that can include a first compliant optrode. The first compliant optrode can include a stack of flexible waveguide materials providing a first optical interface and configured to be introduced into a tissue sample. The stack of flexible waveguide materials can have a thickness of less than about 100 microns. The first compliant optrode can be substantially linear and can be configured to bend at a turn radius of less than about 300 microns.Type: ApplicationFiled: November 13, 2017Publication date: May 17, 2018Inventors: Jesse J. Wheeler, Joseph J. Register, Parshant Kumar, Carlos A. Segura, Charles A. Lissandrello, John J. LeBlanc