Abstract: An improved culture vessel or bioreactor for growing cells, cellular aggregates, tissue particles or tissue “organoids” and methods for using same which includes a housing having at least one chamber defined by a curved wall symmetrical about an axis. The perfused version of the vessel has at least one inlet and at least one outlet and at least one filter in fluid communication with at least one of the inlets or outlets structured to pass culture media but retain cells, cellular aggregates or tissues within the culture chamber. The batch culture version of the vessel has at least part of the vessel wall made of a gas permeable material. The vessel housing is rotated about the horizontal or proximate horizontal axis to suspend the cells, in an unobstructed, three-dimensional or toroidal flow. The chamber has a substantially ellipsoid, oblate ellipsoid, spherical or oblate spherical shape and an unobstructed volume for growth of cells, cellular aggregates or tissues.

Abstract: A bioreactor system for culturing cells, cellular aggregates, tissues and organoids and methods of using same includes a cylindrical reactor vessel having an unobstructed longitudinal axis that is rotated about that horizontal axis to suspend cells and/or tissue in a culture medium. Filters are used within the vessel to retain the cells and/or tissues within the culture chamber and may be also used to subdivide the culture chamber. A pump maintains a flow of oxygen rich culture medium through the vessel to sustain cell respiration. A gas exchange device maintains desired gas concentrations in the medium and the entire system is operated within an enlarged incubator to maintain appropriate temperatures. Access ports on the vessel are used to introduce various materials into the culture chamber and to remove bubbles that may form in the medium. Sensors and a microprocessor unit monitor operational conditions and direct adjustments to maintain desired conditions.

Abstract: A bioreactor system for culturing cells, cellular aggregates, tissues and organoids and methods of using same includes a cylindrical reactor vessel having an unobstructed longitudinal axis that is rotated about that horizontal axis to suspend cells and/or tissue in a culture medium. Filters are used within the vessel to retain the cells and/or tissues within the culture chamber and may be also used to subdivide the culture chamber. A pump maintains a flow of oxygen rich culture medium through the vessel to sustain cell respiration. A gas exchange device maintains desired gas concentrations in the medium and the entire system is operated within an enlarged incubator to maintain appropriate temperatures. Access ports on the vessel are used to introduce various materials into the culture chamber and to remove bubbles that may form in the medium. Sensors and a microprocessor unit monitor operational conditions and direct adjustments to maintain desired conditions.