Patents by Inventor Charles H. Squires
Charles H. Squires has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11944083Abstract: An apparatus for stowing and/or transporting rod-shaped structures such that the rods are easily insertable into, and removable from, the rod holder when it is located underneath an overhead obstruction that would otherwise prevent insertion or removal of rods into the rod holder. The rod holder rotates away from the receiving structure to which it is attached by operation of a rotatable attachment between the rod holder and a base. The base comprises a latching mechanism to prevent the rod holder from rotating unless it is released using a latch release lever. The rod holder allows insertion of longer rods into the rod holder than would otherwise be possible using rod holders of the prior art. The rod holder increases ease of access to rods stored in the rod holder. An exemplary use case is the storage of fishing rods under a T-top on a fishing boat.Type: GrantFiled: October 8, 2021Date of Patent: April 2, 2024Inventors: Michael J. Ottomanelli, Jordan H. Smith, Charles C. Squires
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Patent number: 10689640Abstract: The present invention provides an array for rapidly identifying a host cell population capable of producing a heterologous recombinant protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous recombinant protein of interest in a periplasm compartment or may secrete the heterologous recombinant protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest including therapeutic proteins, hormones, growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.Type: GrantFiled: December 19, 2016Date of Patent: June 23, 2020Assignee: Pfenex Inc.Inventors: Diane M. Retallack, Charles H. Squires, Thomas M. Ramseier, Russell J. Coleman, Jane C. Schneider, Charles D. Hershberger
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Publication number: 20180291412Abstract: The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances. Processes for improved production of isolated mammalian, particularly human, proteins are provided.Type: ApplicationFiled: June 21, 2018Publication date: October 11, 2018Inventors: Diane M. Retallack, Charles H. Squires, David C. Watkins, Stacey L. Lee, Frank H. Gaertner, Robert Shutter
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Patent number: 10041102Abstract: The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances. Processes for improved production of isolated mammalian, particularly human, proteins are provided.Type: GrantFiled: August 5, 2016Date of Patent: August 7, 2018Assignee: PFENEX INC.Inventors: Diane M. Retallack, Charles H. Squires, David C. Watkins, Stacey L. Lee, Frank H. Gaertner, Robert Shutter
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Patent number: 9708616Abstract: Provided herein are methods and compositions for expression of a nucleic acid construct comprising nucleic acids encoding a) a recombinant polypeptide, and b) a prototrophy-restoring enzyme in a host cell that is auxotrophic for at least one metabolite. In various embodiments, the host cell is auxotrophic for a nitrogenous base compound or an amino acid. The invention involves introducing an analog into the growth media for the host cell such that the analog is incorporated into the recombinant polypeptide or a nucleic acid coding sequence thereof. In various embodiments, the compositions and methods disclosed herein result in improved recombinant protein expression compared to expression of recombinant protein in an antibiotic selection system, or compared to expression of the recombinant protein in an expression system that lacks a metabolite analog.Type: GrantFiled: December 18, 2015Date of Patent: July 18, 2017Assignee: PFENEX, INC.Inventors: Diane M. Retallack, Lawrence C. Chew, Charles H. Squires
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Publication number: 20170183646Abstract: The present invention provides an array for rapidly identifying a host cell population capable of producing heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment, or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest, including therapeutic proteins, hormones, a growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.Type: ApplicationFiled: December 19, 2016Publication date: June 29, 2017Inventors: Diane M. Retallack, Charles H. Squires, Thomas M. Ramseier, Russell J. Coleman, Jane C. Schneider, Charles D. Hershberger
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Patent number: 9580719Abstract: The present invention provides an array for rapidly identifying a host cell population capable of producing a heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest including therapeutic proteins, hormones, growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.Type: GrantFiled: October 30, 2009Date of Patent: February 28, 2017Assignee: PFENEX, INC.Inventors: Diane M. Retallack, Charles H. Squires, Thomas M. Ramseier, Russell J. Coleman, Jane C. Schneider, Charles D. Hershberger
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Publication number: 20170051327Abstract: The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable Circumstances Processes for improved production of isolated mammalian, particularly human, proteins are provided.Type: ApplicationFiled: August 5, 2016Publication date: February 23, 2017Inventors: Diane M. Retallack, Charles H. Squires, David C. Watkins, Stacey L. Lee, Frank H. Gaertner, Robert Shutter
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Patent number: 9458487Abstract: The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances Processes for improved production of isolated mammalian, particularly human, proteins are provided.Type: GrantFiled: April 7, 2006Date of Patent: October 4, 2016Assignee: PFENEX, INC.Inventors: Diane M. Retallack, Charles H. Squires, David C. Watkins, Stacey L. Lee, Frank H. Gaertner, Robert Shutter
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Patent number: 9453251Abstract: The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances Processes for improved production of isolated mammalian, particularly human, proteins are provided.Type: GrantFiled: January 18, 2005Date of Patent: September 27, 2016Assignee: PFENEX INC.Inventors: Diane M. Retallack, Charles H. Squires, David C. Watkins, Stacey L. Lee, Frank H. Gaertner, Robert Shutter
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Publication number: 20160208268Abstract: Provided herein are methods and compositions for expression of a nucleic acid construct comprising nucleic acids encoding a) a recombinant polypeptide, and b) a prototrophy-restoring enzyme in a host cell that is auxotrophic for at least one metabolite. In various embodiments, the host cell is auxotrophic for a nitrogenous base compound or an amino acid. The invention involves introducing an analogue into the growth media for the host cell such that the analogue is incorporated into the recombinant polypeptide or a nucleic acid coding sequence thereof. In various embodiments, the compositions and methods disclosed herein result in improved recombinant protein expression compared to expression of recombinant protein in an antibiotic selection system, or compared to expression of the recombinant protein in an expression system that lacks a metabolite analogue.Type: ApplicationFiled: December 18, 2015Publication date: July 21, 2016Inventors: Diane M. Retallack, Lawrence C. Chew, Charles H. Squires
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Patent number: 9394571Abstract: The present invention provides an array for rapidly identifying a host cell population capable of producing a heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest including therapeutic proteins, hormones, growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.Type: GrantFiled: April 25, 2008Date of Patent: July 19, 2016Assignee: PFENEX INC.Inventors: Thomas M. Ramseier, Russell J. Coleman, Jane C. Schneider, Charles D. Hershberger, Diane M. Retallack, Charles H. Squires
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Patent number: 9243253Abstract: Provided herein are methods and compositions for expression of a nucleic acid construct comprising nucleic acids encoding a) a recombinant polypeptide, and b) a prototrophy-restoring enzyme in a host cell that is auxotrophic for at least one metabolite. In various embodiments, the host cell is auxotrophic for a nitrogenous base compound or an amino acid. The invention involves introducing an analogue into the growth media for the host cell such that the analogue is incorporated into the recombinant polypeptide or a nucleic acid coding sequence thereof. In various embodiments, the compositions and methods disclosed herein result in improved recombinant protein expression compared to expression of recombinant protein in an antibiotic selection system, or compared to expression of the recombinant protein in an expression system that lacks a metabolite analogue.Type: GrantFiled: May 10, 2010Date of Patent: January 26, 2016Assignee: Pfenex, Inc.Inventors: Diane M. Retallack, Lawrence C. Chew, Charles H. Squires
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Patent number: 9109229Abstract: This invention is a process for improving the production levels of recombinant proteins or peptides or improving the level of active recombinant proteins or peptides expressed in host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant protein and modifying the cell to change the expression of a gene product that is upregulated in response to the recombinant protein expression. The process can improve protein production or can improve protein quality, for example, by increasing solubility of a recombinant protein.Type: GrantFiled: November 4, 2013Date of Patent: August 18, 2015Assignee: Pfenex Inc.Inventors: Thomas M. Ramseier, Hongfan Jin, Charles H. Squires
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Publication number: 20140162279Abstract: This invention is a process for improving the production levels of recombinant proteins or peptides or improving the level of active recombinant proteins or peptides expressed in host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant protein and modifying the cell to change the expression of a gene product that is upregulated in response to the recombinant protein expression. The process can improve protein production or can improve protein quality, for example, by increasing solubility of a recombinant protein.Type: ApplicationFiled: November 4, 2013Publication date: June 12, 2014Applicant: PFENEX INC.Inventors: THOMAS M. RAMSEIER, HONGFAN JIN, CHARLES H. SQUIRES
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Patent number: 8603824Abstract: This invention is a process for improving the production levels of recombinant proteins or peptides or improving the level of active recombinant proteins or peptides expressed in host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant protein and modifying the cell to change the expression of a gene product that is upregulated in response to the recombinant protein expression. The process can improve protein production or can improve protein quality, for example, by increasing solubility of a recombinant protein.Type: GrantFiled: July 26, 2005Date of Patent: December 10, 2013Assignee: Pfenex, Inc.Inventors: Thomas M. Ramseier, Hongfan Jin, Charles H. Squires
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Publication number: 20110020830Abstract: The present invention provides methods and compositions for the generation and identification of expression constructs that can be used to express sufficient levels of a polypeptide of interest. The compositions include a population of expression vectors, wherein members of the population have a type IIS restriction enzyme recognition site adjacent to a regulatory sequence, and wherein the regulatory element is distinct in at least two members of the population of expression vectors. In various embodiments, the expression vectors further comprise a polynucleotide sequence encoding a polypeptide of interest, wherein the polynucleotide encoding the polypeptide, the polynucleotide of the regulatory sequence, or both, are distinct in at least two members of the population. The compositions are useful for identifying a combination of coding sequences and/or regulatory elements useful for the heterologous expression of the polypeptide of interest.Type: ApplicationFiled: March 31, 2009Publication date: January 27, 2011Inventors: Jane C. Schneider, Charles H. Squires, Huizhu Liu
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Publication number: 20100137162Abstract: The present invention provides an array for rapidly identifying a host cell population capable of producing heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment, or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest, including therapeutic proteins, hormones, a growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.Type: ApplicationFiled: October 30, 2009Publication date: June 3, 2010Applicant: Pfenex Biopharmaceuticals, Inc.Inventors: Diane M. Retallack, Charles H. Squires, Thomas M. Ramseier, Russell J. Coleman, Jane C. Schneider, Charies D. Hershberger
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Patent number: 6274372Abstract: The invention provides a novel flavin reductase, which has been isolated from Rhodococcus strain sp. IGTS8 and purified to homogeneity. The flavin reductase is of use in the biocatalytic desulfurization of a fossil fuel containing organic sulfur compounds. The method comprises the steps of (1) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon—sulfur bonds and a rate-enhancing amount of the flavin reductase of the present invention, thereby forming a fossil fuel and aqueous phase mixture; (2) maintaining the mixture under conditions sufficient for cleavage of the carbon—sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and (3) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase.Type: GrantFiled: May 3, 1999Date of Patent: August 14, 2001Assignee: Enchira Biotechnology CorporationInventors: Kevin A. Gray, John D. Childs, Charles H. Squires
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Patent number: 6235519Abstract: The present invention relates to an isolated nucleic acid molecule which encodes one or more of the enzymes which catalyze one or more steps in the desulfurization of thiophene, or a homologue or active fragment thereof. The invention also includes a recombinant microorganism containing one or more such heterologous nucleic acid molecules. The invention also provides a method for desulfurizing a fossil fuel containing thiophene and/or one or more substituted thiophenes. The method comprises contacting the fossil fuel with an organism containing a recombinant nucleic acid molecule which encodes an enzyme which catalyzes the desulfurization of thiophene.Type: GrantFiled: February 26, 1998Date of Patent: May 22, 2001Assignee: Energy Biosystems CorporationInventors: Yongzhao Wang, John D. Childs, Charles H. Squires