Abstract: Disclosed are nucleic acid segments comprising synthetically-modified genes encoding Coleopteran-toxic B. thuringiensis ?-endotoxins. Also disclosed are methods of using these genes for the recombinant expression of polypeptides, the preparation of vectors containing the genes, and methods for transforming suitable host cells.

Abstract: Disclosed are nucleic acid segments comprising synthetically-modified genes encoding Coleopteran-toxic B. thuringiensis ?-endotoxins. Also disclosed are methods of using these genes for the recombinant expression of polypeptides, the preparation of vectors containing the genes, and methods for transforming suitable host cells.

Abstract: Disclosed are nucleic acid segments comprising synthetically-modified genes encoding Coleopteran-toxic B. thuringiensis ?-endotoxins. Also disclosed are methods of using these genes for the recombinant expression of polypeptides, the preparation of vectors containing the genes, and methods for transforming suitable host cells.

Abstract: Disclosed are nucleic acid segments comprising synthetically-modified genes encoding Coleopteran-toxic B. thuringiensis ?-endotoxins. Also disclosed are methods of using these genes for the recombinant expression of polypeptides, the preparation of vectors containing the genes, and methods for transforming suitable host cells.

Abstract: Methods are described for dilating biological conduits by removing elastin and remodeling collagens in the wall of the conduit. Methods include the use of agents that increase the release of endogenous elastase and collagenase in the wall of the conduit, either by cells that are normally present in the wall of the conduit or by inflammatory cells that are attracted to the conduit, thereby providing additional conduit dilation. Methods also include the use of agents that increase conduit wall permeability and expose elastin and collagen fibers. Methods also include removing components of the extracellular matrix of arteries and veins leading to an inhibition of intimal hyperplasia in the wall of the vessels by decreasing biomechanical stimuli directed toward the cells in the wall of the vessel. Methods further include the use of agent that degrade micro fibers, in addition to elastin, in order to decrease the resynthesis of elastin.

Abstract: Disclosed are nucleic acid segments comprising synthetically-modified genes encoding Coleopteran-toxic B. thuringiensis ?-endotoxins. Also disclosed are methods of using these genes for the recombinant expression of polypeptides, the preparation of vectors containing the genes, and methods for transforming suitable host cells.

Abstract: Expressed Sequence Tags (ESTs) isolated from the Western Corn Rootworm, Diabrotica virgifera virgifera LeConte, are disclosed. The invention encompasses nucleic acid molecules that encode D. v. virgifera protein homologs and fragments thereof. In addition, antibodies capable of binding the proteins are encompassed by the present invention. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, and antibodies, for example, for gene identification and analysis, and preparation of constructs.

Abstract: Disclosed is a means of controlling plant pests by a novel method of expressing Cry2A B. thuringiensis ?-endotoxins in plants. The invention comprises novel nucleic acid segments encoding proteins comprising Cry2A B. thuringiensis ?-endotoxins. The nucleic acid segments are disclosed, as are transformation vectors containing the nucleic acid segments, plants transformed with the claimed segments, methods for transforming plants, and methods of controlling plant infestation by pests.

Abstract: The present invention describes compositions and methods that are useful in the control of lepidopteran insect pests, and more particularly describes nucleotide sequences for use in plants that encode full-length and truncated insecticidal toxins, as well as chimeric toxins. The nucleotide sequences of the present invention exhibit modifications that, when compared to the native sequences obtained from Bacillus thuringiensis species, make them particularly useful for enhanced, improved, and or optimized expression in monocot and dicot plant species. Using methods well known to those skilled in the art the nucleotide sequences described herein can be used to transform plant cells and plant tissue in order to produce transgenic plants that express the encoded proteins, therefore conferring upon the transgenic plants the ability to resist insect infestation.

Abstract: An inkjet recording element comprising a support having thereon, in order over a support, a base layer comprising a synthetic or natural polymer and optionally a polymeric mordant; an inner layer comprising acetoacetylated or carboxylated poly(vinyl alcohol); and an overcoat comprising poly(vinyl alcohol) binder and particles of a synthetic, substantially amorphous aluminosilicate material. Such recording elements exhibit improved adhesion during printing.

Abstract: An inkjet recording element comprising a support having thereon, in order, a base layer comprising a hydrophilic polymer and a polymeric mordant, an inner layer, and an overcoat. Such recording elements exhibit improved resistance to humidity keeping.

Abstract: An inkjet recording element comprising a support having thereon, in order over a support, at least three hydrophilic absorbing layers, including a base layer comprising a synthetic or natural polymer and optionally a polymeric mordant; an inner layer comprising a poly(vinyl alcohol) binder and particles of synthetic, substantially amorphous aluminosilicate material; and an overcoat comprising a poly(vinyl alcohol) binder and particles of synthetic, substantially amorphous aluminosilicate material. Such recording elements exhibit improved interlayer adhesion during printing.

Abstract: The present invention discloses methods and compositions comprising a group of novel expression cassettes which provide significantly improved levels of accumulation of Coleopteran inhibitory Cry3B and Cry3B variant amino acid sequences when these are expressed in plants. The preferred embodiments of the invention provide at least up to ten fold higher levels of insect controlling protein relative to the highest levels obtained using prior compositions. In particular, transgenic maize expressing higher levels of a protein designed to exhibit increased toxicity toward Coleopteran pests deliver superior levels of insect protection and are less likely to sponsor development of populations of target insects that are resistant to the insecticidally active protein.

Abstract: Methods and compositions are provided for screening candidate antiviral agents using cells containing subgenomic viral replication systems such as replicons and minigenomes. The methods involve the simultaneous assay of more than one subgenomic viral replication system. Compositions useful for these methods are also provided.

Abstract: An inkjet recording element comprising a support having thereon, in order, a support having thereon an ink-receiving layer comprising a hydrophilic polymer, a polymeric mordant, and particles of an aluminosilicate. Such recording elements exhibit improved humidity keeping.

Abstract: An inkjet recording element comprising a support having thereon a subbing layer comprising particles of an aluminosilicate for improved adhesion.

Abstract: An inkjet recording element comprising a support having thereon, in order, a support having thereon a ink-receiving layer comprising a hydrophilic polymer and particles of an aluminosilicate in an amount of less than 30 weight percent solids, based on the total weight of the layer. Such recording elements exhibit improved humidity keeping for print sharpness.

Abstract: An ink jet recording element comprising a support having thereon, in order, a hydrophilic absorbing layer and a polymeric overcoat layer comprising particles of a substantially amorphous synthetic aluminosilicate. Such recording elements exhibit improved humidity keeping and smudge resistance, while maintaining good differential gloss.

Abstract: Disclosed are nucleic acid segments comprising synthetically-modified genes encoding Coleopteran-toxic B. thuringiensis &dgr;-endotoxins. Also disclosed are methods of using these genes for the recombinant expression of polypeptides, the preparation of vectors containing the genes, and methods for transforming suitable host cells.

Abstract: The invention provides transgenic plants and transformed host cells which express modified cry 3B genes with enhanced toxicity to Coleopteran insects. Also disclosed are methods of making and using these transgenic plants, methods of making recombinant host cells expressing these ?-endotoxins, and methods of killing insects such as Colorado potato beetle (Leptinotarsa decemlineata), southern corn rootworm (Diabrotica undecimpunctata howardi Barber) and western corn rootworm (Diabrotica virgifera virgifera LeConte.