Abstract: The present disclosure relates a method of fabricating a literal flow immunoassay (LFIA) for the diagnosis of diseases, including COVID-19. The present disclosure further relates to a fusion-epitopes peptide that can be used in the LFIA test to improve sensitivity, specificity and accuracy of the test.

Abstract: Described are nanogels suitable for scaffolds for encapsulating human dermal fibroblasts for non-healing chronic wounds. Peptide nanogels Ac-IVZK-NH2 and Ac-IVFK-NH2 are selected and produce silver nanoparticles in situ within the nanogels to assess their efficacy on micropigs with full-thickness excision wounds. The in situ generation of the silver nanoparticles is done solely through UV irradiation and no reducing agent is used. Application of the peptide nanogels on full thickness micropig wounds demonstrate that the scaffolds are biocompatible and do not trigger wound inflammation. This suggests that scaffolds are safe for topical application. A comparison of the effect of both nanogels even without the addition of the silver nanoparticles, reveals that the scaffold itself has a high potential as an antibacterial agent, which may suppress both the inflammatory reaction and the activity of proteases. The effect on wound closure of the peptide nanogels is comparable to standard care hydrogels.

Abstract: The present disclosure relates a method of fabricating a literal flow immunoassay (LFIA) for the diagnosis of diseases, including COVID-19. The present disclosure further relates to a fusion-epitopes peptide that can be used in the LFIA test to improve sensitivity, specificity and accuracy of the test.

Abstract: A facile strategy is used to synthesize the gold nanoparticles via a green and simple approach showing self-alignment on the assembled nanofibers of ultrashort oligopeptides as a composite material. A photochemical reduction method is used, without requiring any external chemical reagents for the reduction of gold ions and producing gold nanoparticles of size ca. 5 nm under mild UV light exposure. The specific arrangement of gold nanoparticles on peptide nanofibers may indicate electrostatic interactions of two components and interactions with the amino group of the peptide building block. The gold-peptide nanoparticle composites show the ability as a catalyst to degradation of environmental pollutant p-nitrophenol to p-aminophenol, and the reaction rate constant for catalysis is 0.057 min?1 at a 50-fold dilute sample of 2 mg/mL and 0.72 mM gold concentration in the composites.

Abstract: Newly developed peptide nanogels provide native cues to human dermal fibroblasts as well as mouse myoblast cells and promote proliferation and extensive network formation in vitro is presented. The results represent an improvement in the fabrication of dermal grafts as well as 3D skin models. In addition, the application of these ultrashort peptide nanogels on full-thickness wounds in a minipig model demonstrated biocompatibility with the minipig skin tissue, as the peptide nanogels did not trigger wound inflammation. Thus, they can be considered as a safe biomaterial for topical applications. It is shown that both peptides are printable. The ability to print peptides and the return of high cell viability within the printed construct will open up the possibility of 3D bioprinting of different cell types in future.

Abstract: Described is a biomaterial/carbonate-based ink that comprises a biopolymer-based mixture and bioceramics. The photo- and ionic crosslinkable biopolymer mixture comprises polysaccharide and gelatin-based materials. The bioceramics comprises an apatite and a carbonate. The biopolymer-based mixture is mixed with the bioceramics to form the ink. The ink is capable of being applied under wet or dry conditions. The wet condition is seawater or water or other aqueous solution. The ink is capable to instantly get solidified, when UV or blue light is applied in the presence of the ionic components found in seawater. After photo- or ionic crosslinking, this ink is stable for months.

Abstract: A modified approach for coral restoration by merging 3D printing and molding techniques is presented. This is achieved by 3D scanning live coral specimens, retrieved from sea dives, to obtain a CAD model of the complete coral 3D construction with complex geometries. Select areas of the model are flattened to create a 2D base for micro-fragment adhesion. From the CAD models, disclosed embodiments propose two methods of fabrication. Method A consists of 3D printing the CAD models with commercial thermoplastic materials to create a negative mold, subsequently loaded with synthesized Calcium Carbonate Photoinitiated (CCP) ink to form an eco-friendly coral skeleton. Method B uses syringe-based extrusion 3D printing to directly print a coral skeleton with CCP ink. Both methods are evaluated as a combined proof-of-concept process, 3D CoraPrint, for coral gardening and restoration and providing details required for mimicking coral and bone 3D structures for implantation in bone grafting applications.

Abstract: The present disclosure relates to a cell-laden microgel comprising self-assembly ultrashort peptide (SUP) and a method of frabricating such cell-laden microgels. The present disclosure also relates to a cell microcarrier comprising cell-laden microgels, which is suitable for medical applications such as cell therapy. The present disclosure further relates to a system comprising a combination of SUP microgel and SUP bulk hydrogel for vascularized tissue culture and a method of creating such a vascularized 3D tissue constructs with improved cell viability and proliferation.

Abstract: The present disclosure relates to Dopa containing ultrashort peptides capable of forming a gel, to a gel comprising a peptide in accordance with the present disclosure, and to a glue comprising a peptide in accordance with the present disclosure. Such gel is adhesive and is biocompatible. The peptides are suitable for building 3D structures, 3D printing, gluing as well as other applications.

Abstract: The present invention relates to a functional 3D neuronal model based on ultrashort self-assembling peptide scaffolds in accordance with the present invention, and to a method of preparing such a model. The models are suitable for in vitro drug testing, cellular replacement therapies as well as other applications.

Abstract: The present invention relates to a device and a method for building a 3D object by mixing a bioink solution, a buffer solution capable of inducing gelation of the bioink solution and a dispersion containing micro and/or nanoparticles, and ejecting the formed hydrogel out of a nozzle. The present invention further relates to a method of obtaining a hydrogel.

Abstract: The present disclosure relates to a coaxial nozzle for building a 3D tissue model. Accordingly, a nozzle for 3D tissue bioprinting may include at least one buffer solution inlet, at least one peptide inlet, at least one cell inlet, and a mixing chamber. The least one buffer solution inlet is attached to the at least one peptide inlet. The at least one cell inlet is attached to the at least one peptide inlet. The at least one buffer solution inlet may be attached from a side of the at least one peptide inlet, and the at least one cell inlet may be disposed externally and attached at an angle to the at least one peptide inlet.

Abstract: Portable and interchangeable marine tiles, marine pods and tanks are disclosed. The marine tile typically contains a first face, a second face, and one or more sites for supporting one or more marine organisms, such as coral, and. may contain a tracking device. The marine pod typically contains a first panel having a first slit and a second panel having a second slit. The first panel and the second panel can be interlocked via sliding the first slit into the second slit. The marine pod can have one or more marine tiles attached thereto. The marine tank typically contains one or more coral reef organisms. Water can be pumped into and through the marine tank to flow in the form of a river stream. Typically, the flow of the seawater is controlled such that a consistent temperature is maintained in the marine tank.

Abstract: Compositions for making native-like marine structures are provided. The compositions include materials, typically a limestone and/or ceramic composite, for 3D printing of marine structures. Environmentally-friendly 3D printing and coating methods are also provided. The methods can be used to print structures/objects composed of a 3D printing material such as the compositions described herein, and/or coat formed structures/objects with an adhesive, such as a peptide-based bioorganic adhesive. Compositions and methods for supporting the attachment and growth of marine organisms such as coral to a substrate are also provided. The compositions contain one or more self-assembling peptides suitable for use as bioorganic adhesives. The peptide compositions are suitable for adhesive applications in the marine environment such as supporting coral growth and restoration. Methods of using the peptide-based adhesive compositions are also provided.

Abstract: The present invention relates to peptides capable of forming a gel and to their use in tissue engineering and bioprinting. The present invention furthermore relates to a gel comprising a peptide in accordance with the present invention, to a method of preparing such gel and to the use of such gel. In one embodiment, such gel is a hydrogel. The present invention furthermore relates to a wound dressing or wound healing agent comprising a gel according to the present invention and to a surgical implant or stent comprising a peptide scaffold formed by a gel according to the present invention. Moreover, the present invention also relates to a pharmaceutical and/or cosmetic composition, to a biomedical device or an electronic device comprising the peptide according to the present invention.

Abstract: The present disclosure relates generally to an osteo-tissue graft capable of promoting bone tissue growth and regeneration, comprising at least one self-assemble peptide and mesenchymal stem cells (MSCs) in accordance with the present invention and a method of preparing such an osteo-tissue graft. The grafts are suitable for treatment of bone disorder or damages through tissue engineering, cellular replacement therapies as well as other applications.

Abstract: The present disclosure relates to a coaxial nozzle for building a 3D tissue model. Accordingly, a nozzle for 3D tissue bioprinting may include at least one buffer solution inlet, at least one peptide inlet, at least one cell inlet, and a mixing chamber. The least one buffer solution inlet is attached to the at least one peptide inlet. The at least one cell inlet is attached to the at least one peptide inlet. The at least one buffer solution inlet may be attached from a side of the at least one peptide inlet, and the at least one cell inlet may be disposed externally and attached at an angle to the at least one peptide inlet.

Abstract: The present disclosure relates to ultrashort peptides capable of forming a gel, to a gel comprising a peptide in accordance with the present disclosure, and to a method of preparing such gel. Such gel is a hydrogel or an organogel. The peptides are suitable bioinks for a bioprinter to build 3D structures through 3D printing as well as other applications.