Abstract: An electrochemical method and a test strip for detecting hemoglobin in a specimen are provided. The method includes the steps of providing the specimen with a reagent including a buffer solution, a surfactant and an electron mediator, tetrathiafulvalene, modified by cyclodextrin; detecting electric current produced by reaction of the hemoglobin and the electron mediator in the specimen under a potentiostatic condition; and calculating a concentration of the hemoglobin in the specimen according to the detected electric current.

Abstract: The present invention provides an electrochemical method and a test strip for detecting hemoglobin in a specimen. The method includes the steps of providing the specimen with a reagent, which has a buffer solution, a surfactant and an electron mediator, tetrathiafulvalene, modified by cyclodextrin; detecting electric current produced by the reaction of the hemoglobin and the electron mediator in a specimen under a potentiostatic condition; and calculating a hemoglobin concentration in the specimen according to the detected electric current. In the method of the present invention, tetrathiafulvalene modified by cyclodextrin is used as an electron mediator, which directly interacts with the hemoglobin in the specimen.

Abstract: An electrochemical immuno diagnostic strip and its preparation method are disclosed herein. A screen-printed electrode is prepared on an insulating substrate, which is divided into an electrode area and a connecting area. An electrochemical precursor is placed on the electrode area and a reaction membrane is provided on the electrode area. A specimen loading area is at the reaction membrane. The present invention is advantageous to overcome the interference problem of the electrochemical detection technology and to provide the quantitative analysis of the prompt diagnostic strip.

Abstract: A sensing member for detecting total cholesterol of a blood sample is disclosed, which includes: a working electrode and a counter electrode respectively formed on a first substrate and a second substrate, wherein, an opening is formed in one of the first and second substrates corresponding in position to the electrode for allowing the blood sample to pass through; a dielectric layer formed on one of the surfaces of the first and second substrates and exposing the electrode and end connector such that the end connector can be electrically connected to an electronic device used for applying electric voltage; a reactive pad disposed between the working electrode and the counter electrode and containing an reactive reagent, wherein the reactive reagent comprises an electron transport material, an enzyme capable of reacting with the analyte and generating a current corresponding to the concentration, and a buffer solution; and a separating pad disposed on the opening for removing interfering materials from the bl

Abstract: An electrochemical biosensor formed by screen printing and method of fabricating such biosensor is disclosed in the present invention. The biosensor can quickly absorb a sample to be measured therein, effectively control volume of the sample fed and “fill-and-position” the sample therein. The biosensor includes an electrode layer (electrode area) comprising two or three electrodes, which are a working electrode, a reference electrode and an auxiliary electrode (tri-electrode) on an insulating substrate. An active reaction layer containing reactant, reaction catalyst, mediator, wetting agent and surfactant is spread on the surface of the electrode layer. A sample inflow area is formed above the electrode area by adding an upper cover on top of a middle insulating layer with a U-shaped opening formed therein. Sample solution with a minute amount about 0.

Abstract: An electrochemical biosensor formed by screen printing and method of fabricating such biosensor is disclosed in the present invention. The biosensor can quickly absorb a sample to be measured therein, effectively control volume of the sample fed and “fill-and-position” the sample therein. The biosensor includes an electrode layer (electrode area) comprising two or three electrodes, which are a working electrode, a reference electrode and an auxiliary electrode (tri-electrode) on an insulating substrate. An active reaction layer containing reactant, reaction catalyst, mediator, wetting agent and surfactant is spread on the surface of the electrode layer. A sample inflow area is formed above the electrode area by adding an upper cover on top of a middle insulating layer with a U-shaped opening formed therein. Sample solution with a minute amount about 0.

Abstract: Disclosed is an automatic computer-controlled bioreactor system for enzymatic synthesis of L-tryptophan which comprises a bioreactor linked to a computer equipped with an on-line indole assay device, in which the computer controls the feeding of indole, pyruvic acid and/or its salt and an ammonium salt into the bioreactor and feedback-controls the reaction in the bioreactor by indole concentration determined by the on-line indole assay device, wherein the feeding of indole and ammonium salt is controlled by a pre-defined profile that agrees with a tryptophanase activity profile and pyruvic acid and/or its salt is added at a predetermined time.

Abstract: Disclosed is a process for the preparation of N-benzyloxycarbonyl-.alpha.-L-aspartyl-L-phenylalanine methyl ester wherein a protease is used to catalyze the condensation reaction of N-benzyloxycarbonyl-L-aspartic acid with L-phenylalanine methyl ester in a system consisting of ethyl acetate, citrate buffer with the addition of glycerol as a dispersing agent.

Abstract: A method of recovering and separating water-soluble cyclodextrins from cyclodextrin formation liquid, wherein the CD formation liquid or the CD liquid after separating .beta.-CD by concentrated precipitation method is mixed with .alpha.-amylase to hydrolyze dextrins to small oligosaccharides, then is concentrated and precipitated again to separate the remained .beta.-CD, after that, soluble .alpha.- and .gamma.-CD are recovered by using inclusion extraction method with organic solvent from the residual liquid.