Abstract: The present invention provides sample devices. The present invention also provides in vivo drug screening systems. The present invention further provides a method for in vivo drug screening.

Abstract: The present invention utilizes multiphoton microscopy for a quantitative analysis of a bio tissue or a skin. Multiphoton microscopy is characterized in low invasion, low photo damage and high penetration. Hence, scanning through multiphoton microscopy does not hurt the bio tissue. It is thus fit for scanning live bio tissues to know its aging status. In addition, the quantitative analysis provides a precise index for diagnosing a damage severity of the tissue, like cancer.

Abstract: The present invention provides imaging devices. The present invention also provides in vivo drug screening systems. The present invention further provides a method for in vivo drug screening.

Abstract: A multiphoton excitaion microscope for simultaneously detecting differently colored fluorescence materials on biochips includes a multiphoton excitation source, objectives, and a plurality of detection channels. The biochip is hybridized and labeled with fluorescence materials for expressing hybridized biological signals. The multiphoton excitation source is focused to a light spot on the biochip to excite the fluorescence materials bound thereon. After that, the fluorescence emission at different wavelengths from the different fluorescent materials can be detected by the plural detection channels.

Abstract: This invention provides a multiphoton excitaion microscope for simultaneously detecting differently colored fluorescence materials on biochips, which includes a multiphoton excitation source, objectives, and a plurality of detection channels. The biochip is hybridized and labeled with fluorescence materials for expressing hybridized biological signals. The multiphoton excitation source is focused to a light spot on the biochip to excite the fluorescence materials bound thereon. After that, the fluorescence emission at different wavelengths from the different fluorescent materials can be detected by the plural detection channels.

Abstract: The invention features a microscopy system for imaging a sample including: a substantially transparent optical block having an interface for positioning the sample adjacent the interface; a light source which during operation overlaps at least two optical beams at the interface, the source directing the beams into the block towards the interface at incident angles that cause the beams to reflect from the interface and establish an evanescent standing wave of electromagnetic energy that extends away from the interface and into the sample; and a detector which during operation records an image of the sample based on optical radiation emitted from the sample in response to the evanescent standing wave.

Abstract: A scanning fluorescence lifetime microscope measures modulation and phase in fluorescence emission stimulated by spatially overlapped pump and probe beams operating at different frequencies. A pump laser modulated at a first frequency is focused onto a diffraction limited spot to excite a fluorescent sample under study. Simultaneously, a probe laser modulated at a second frequency is focused onto the same spot to induce a stimulated fluorescence emission in response to the optically combined output of the pump and probe laser light. Fluorescence emitted from the sample produces a cross-correlation signal which is dependent upon the spatial overlapping of the pump and probe beams at the focal point thereby producing a beneficial axial sectioning effect. Choosing a small differency frequency between the modulation of the first and second laser sources produces a low frequency cross correlation signal even when the modulation frequencies of the pump and probe lasers are very high.