Abstract: Disclosed are calibration techniques that can be implemented by a device that conducts biological tests. In certain embodiments, the device for testing a biological specimen includes a receiving mechanism to receive a carrier, a camera module arranged to capture imagery of the carrier, and a processor. Some examples of the processor can detect a calibration mode trigger. In calibration mode, the processor can divide the captured imagery into segments and selectively perform one or more calibration procedures for each segment. Then, the processor records a calibration result for each segment.

Abstract: Disclosed are verification techniques that can be implemented by a device that conducts biological tests to verify that the specimen holding area carries a valid biological specimen. In certain embodiments, the testing device includes a receiving mechanism to receive a carrier, and the carrier includes a holding area that is to carry or to be exposed to a biological specimen. The device can also include a camera module arranged to capture imagery of the carrier, and a processor. In some examples, the processor can capture the imagery of the carrier and identify a visual cue on the carrier. Then, the processor can verify, based on a manner of how the visual cue is displayed in the captured imagery, whether the holding area carries a valid biological specimen.

Abstract: A sperm sorting device suitable for selectively isolating a plurality of motile sperms from a semen specimen includes a base whose bottom wall and surrounding wall define a receiving space, and a sorter securely coupled to the base and including a mounting member and a sorting filter. The mounting member has a sleeve located in the receiving space and an injection portion located at the bottom end of the sleeve and having an injection hole. The sorting filter is fixed on the sleeve and, together with the injection portion and the sleeve, defines a sorting chamber with an open top end. The sorting filter, the bottom wall, and the surrounding wall define a specimen chamber for receiving the semen specimen. The injection hole brings the specimen chamber and the sorting chamber into communication vertically, so the semen specimen can be injected through the injection hole into the specimen chamber rapidly.

Abstract: Disclosed herein are methods for the detection of the presence of sperm DNA fragmentation in a semen sample. The methods include embedding of sperm cells of the semen sample in a gel, denaturing DNA of the sperm cells, and lysing the nuclear proteins of the sperm cells. The present method includes an ionic surfactant sodium dodycyl sulfate (SDS) and a chaotropic agent urea in the lysis solution for releasing DNA from protamine of chromosome, which significantly reduces the time required for lysis. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.

Abstract: Disclosed herein are methods for the detection of the presence of sperm DNA fragmentation in a semen sample. The methods include embedding of sperm cells of the semen sample in a gel, denaturing DNA of the sperm cells, and lysing the nuclear proteins of the sperm cells. The present method includes an ionic surfactant sodium dodycyl sulfate (SDS) and a chaotropic agent urea in the lysis solution for releasing DNA from protamine of chromosome, which significantly reduces the time required for lysis. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.

Abstract: Disclosed herein is a method for the detection of the presence of sperm DNA fragmentation in a semen sample. The method comprises a step of embedding the semen sample containing sperm cells in a gel comprising acrylamide, acrylic acid, methacrylic acid, N-isopropylacrylamide (NIPAM), alginate, or polyethylene glycol (PEG), to obtain a sperm cells-embedded gel. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.

Abstract: A sperm sorting device suitable for selectively isolating motile sperms from a semen specimen includes a base and a sorting unit. The base comprises a central protruding block including a truncated cone-shaped surface, a bottom wall that surrounds the central protruding block, and an intermediate ring. The intermediate ring connects to the bottom wall via an inner wall surface. The sorting unit includes a sorting ring and a protruding ring. The sorting ring includes a central hole for a sorting filter that filters the semen specimen and is beveled at an angle that increases a permeable surface of the sorting filter. The protruding ring connects to the sorting ring via an inner sleeve surface. The sperm sorting device enhances sperm sorting quality by allowing the motile sperms to swim upward through the first sorting filter naturally.

Abstract: Disclosed herein are methods for the detection of the presence of sperm DNA fragmentation in a semen sample. The methods include embedding of sperm cells of the semen sample in a gel, denaturing DNA of the sperm cells, and lysing the nuclear proteins of the sperm cells. The present method includes an ionic surfactant sodium dodycyl sulfate (SDS) and a chaotropic agent urea in the lysis solution for releasing DNA from protamine of chromosome, which significantly reduces the time required for lysis. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.

Abstract: Disclosed herein is a method for the detection of the presence of sperm DNA fragmentation in a semen sample. The method comprises a step of embedding the semen sample containing sperm cells in a gel comprising acrylamide, acrylic acid, methacrylic acid, N-isopropylacrylamide (NIPAM), alginate, or polyethylene glycol (PEG), to obtain a sperm cells-embedded gel. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.

Abstract: Disclosed are verification techniques that can be implemented by a device that conducts biological tests to verify that the specimen holding area carries a valid biological specimen. In certain embodiments, the testing device includes a receiving mechanism to receive a carrier, and the carrier includes a holding area that is to carry or to be exposed to a biological specimen. The device can also include a camera module arranged to capture imagery of the carrier, and a processor. In some examples, the processor can capture the imagery of the carrier and identify a visual cue on the carrier. Then, the processor can verify, based on a manner of how the visual cue is displayed in the captured imagery, whether the holding area carries a valid biological specimen.

Abstract: Disclosed are calibration techniques that can be implemented by a device that conducts biological tests. In certain embodiments, the device for testing a biological specimen includes a receiving mechanism to receive a carrier, a camera module arranged to capture imagery of the carrier, and a processor. Some examples of the processor can detect a calibration mode trigger. In calibration mode, the processor can divide the captured imagery into segments and selectively perform one or more calibration procedures for each segment. Then, the processor records a calibration result for each segment.

Abstract: Embodiments include a device for testing biological specimen. The device can include a receiving mechanism to receive a carrier. The carrier may include a holding area. The device includes a camera to capture a plurality of images of the holding area. A processor in the device may utilize the camera module to: adaptively select, based on the plurality of images of the holding area, an analytical algorithm suitable for a motion property that is characteristic of the biological specimen being tested; and perform a set of analytic processes that corresponds to the selected analytic algorithm on the captured imagery to generate an analytic result associated with the biological specimen.

Abstract: A collection bottle and a method for detecting a characteristic of a sample are provided. The collection bottle includes a body and a cover. The body of the collection bottle has an accommodating space. The cover of the collection bottle is removably assembled to the body of the collection bottle, and the cover includes a channel and a testing part. The channel of the collection bottle communicates the accommodating space and the outside environment. While the cover of the collection bottle is assembled to the body, the testing part extends toward the accommodating space.

Abstract: Embodiments include a device for testing biological specimen. The device can include a receiving mechanism to receive a carrier. The carrier may include a holding area. The device may include a camera arranged to capture a plurality of images, including a first image and a second image, of the holding area. The device may include a positioning mechanism operable to adjust a relative location of the carrier to the camera. A processor in the device may utilize the camera module to: identify an edge of the first image; cause the positioning mechanism to adjust the relative location of the carrier to the camera in a manner such that, when the camera takes the second image, an edge of the second image aligns with the identified edge of the first image; and perform a set of analytic processes on a combined image from the first and second images.

Abstract: Embodiments include a device for testing biological specimen. The device can include a receiving mechanism to receive a carrier. The carrier may include a holding area. The device may include a camera arranged to capture a plurality of images, including a first image and a second image, of the holding area. The device may include a positioning mechanism operable to adjust a relative location of the carrier to the camera. A processor in the device may utilize the camera module to: identify an edge of the first image; cause the positioning mechanism to adjust the relative location of the carrier to the camera in a manner such that, when the camera takes the second image, an edge of the second image aligns with the identified edge of the first image; and perform a set of analytic processes on a combined image from the first and second images.

Abstract: Embodiments include a device for testing biological specimen. The device can include a receiving mechanism to receive a carrier. The carrier may include a holding area. The device includes a camera to capture a plurality of images of the holding area. A processor in the device may utilize the camera module to: adaptively select, based on the plurality of images of the holding area, an analytical algorithm suitable for a motion property that is characteristic of the biological specimen being tested; and perform a set of analytic processes that corresponds to the selected analytic algorithm on the captured imagery to generate an analytic result associated with the biological specimen.

Abstract: Embodiments disclose a device for testing biological specimen. The device includes a receiving mechanism to receive a carrier. The carrier includes a holding area that carries or has been exposed to the biological specimen. The device includes a camera module arranged to capture imagery of the holding area. The camera module includes an focusing motor operable to adjust a focal point of the camera. The device also includes a processor that is configured to utilize the camera module to determine, based on operations of the focusing motor, a volumetric property of the holding area and perform a set of analytic processes on at least a portion of the captured imagery of the holding area to determine one or more properties of the biological specimen.

Abstract: A biosensor strip includes a strip body, a working electrode and a chemical reagent layer, wherein the strip body has a conductive portion and a reaction channel. The partial wall that forms the reaction channel is a partial or an entire surface layer of the conductive portion. The reaction channel has an opening that receives the sample fluid and an electrode hole. The working electrode is embedded in the electrode hole. The chemical reagent is configured in the reaction channel and generates an electrochemical reaction with the analyte. Therefore, after the sample fluid covers the reaction channel and the working electrode via the opening, the conductive portion and the working electrode on the wall of the reaction channel generate sensing currents and output the signals via the first and second signal output sides to determine an analyte concentration in the sample fluid.

Abstract: Embodiments disclose a device for testing biological specimen. The device includes a receiving mechanism to receive a carrier. The carrier includes a holding area that carries or has been exposed to the biological specimen. The device includes a camera module arranged to capture imagery of the holding area. The camera module includes an focusing motor operable to adjust a focal point of the camera. The device also includes a processor that is configured to utilize the camera module to determine, based on operations of the focusing motor, a volumetric property of the holding area and perform a set of analytic processes on at least a portion of the captured imagery of the holding area to determine one or more properties of the biological specimen.