Abstract: Methods are provided for detection of concentrations of less than 3.4 picomolar of adenosine triphosphate (ATP) using recombinant luciferase in the luciferin-luciferase reaction. Aspects include a low pH composition for use in detecting the presence of ATP. The low pH composition can include low concentrations of detergents and can be used in combination with methods for reading, calculating and interpreting luminescence generated by the ATP-luciferin-luciferase reaction.

Abstract: Methods are provided for detection of concentrations of less than 3.4 picomolar of adenosine triphosphate (ATP) using recombinant luciferase in the luciferin-luciferase reaction. Aspects include a low pH composition for use in detecting the presence of ATP. The low pH composition can include low concentrations of detergents and can be used in combination with methods for reading, calculating and interpreting luminescence generated by the ATP-luciferin-luciferase reaction.

Abstract: Methods are provided for sensitive detection of adenosine triphosphate (ATP) in samples using the luciferin-luciferase reaction. Aspects include using a pH composition that maximizes a signal to noise ratio. The maximum signal to noise ratio can be particularly useful with recombinant luciferase including recombinant Coleoptera luciferase.

Abstract: Methods are provided for sensitive detection of adenosine triphosphate (ATP) in samples using the luciferin-luciferase reaction. Aspects include using a pH composition that maximizes a signal to noise ratio. The maximum signal to noise ratio can be particularly useful with recombinant luciferase including recombinant Coleoptera luciferase.

Abstract: Methods, devices and systems are provided for detection of adenosine triphosphate (ATP) in samples using the luciferin-luciferase reaction. Aspects include a low pH composition for use in detecting the presence of ATP. The low pH composition can include low molarity buffers and detergents and can be used in combination with methods for reading, calculating and interpreting luminescence generated by the ATP-luciferin-luciferase reaction. Both the low molarity and low pH composition, and the methods for reading, calculating and interpreting luminescence, can be used with a single service hygiene monitoring format.

Abstract: A test method and test device for hygiene monitoring of a test sample from a biological material or a surface with a biological material to detect the presence of phosphate and glucose as a measure of hygiene by employing a swab-type, puncturable-membrane test apparatus or a lateral-flow or capillary-flow test apparatus.

Abstract: Methods, devices and systems are provided for detection of adenosine triphosphate (ATP) in samples using the luciferin-luciferase reaction. An aspect of the invention includes a low molarity and low pH composition for use in detecting the presence of ATP. The low molarity and low pH composition can be used in combination with methods for reading, calculating and interpreting luminescence generated by the ATP-luciferin-luciferase reaction. Both the low molarity, low pH composition and the methods for reading, calculating and interpreting luminescence can be used with a single service hygiene monitoring format.

Abstract: A test method and test device for hygiene monitoring of a test sample from a biological material or a surface with a biological material to detect the presence of phosphate and glucose as a measure of hygiene by employing a swab-type, puncturable-membrane test apparatus or a lateral-flow or capillary-flow test apparatus.