Abstract: Provided is a microfluidic device comprising an incubation layer, the incubation layer including at least one dock, each of the at least one dock defines a stepped tank comprising an upper tank and a lower tank, an inflow channel in fluid communication with the stepped tank for supplying a fluid to the stepped tank, and an outflow channel in fluid communication with the stepped tank for draining the fluid from the stepped tank, wherein the geometry of the upper tank is configured to allow culturing of a fish larva therein, and wherein the geometry of the lower tank is configured to reversibly receive the fish larva from the upper tank and to dock the fish larva at a controlled orientation for imaging or observation.

Abstract: Provided is a microfluidic device comprising an incubation layer, the incubation layer including at least one dock, each of the at least one dock defines a stepped tank comprising an upper tank and a lower tank, an inflow channel in fluid communication with the stepped tank for supplying a fluid to the stepped tank, and an outflow channel in fluid communication with the stepped tank for draining the fluid from the stepped tank, wherein the geometry of the upper tank is configured to allow culturing of a fish larva therein, and wherein the geometry of the lower tank is configured to reversibly receive the fish larva from the upper tank and to dock the fish larva at a controlled orientation for imaging or observation.

Abstract: A microfluidic system for separating an analyte from a sample fluid including a series of fluidic channels including at least one first region and at least one second region. The first region includes a plurality of L-nodes, which connects to each other in series. The second region includes a plurality of R-nodes, which connects to each other in series. The first region is configured to trigger at least about one lamination process cycle for both the sample fluid and the buffer fluid and the second region is configured to trigger at least about one reverse lamination process cycle for both the sample fluid and the buffer fluid, whereby the lamination process cycle and the reverse lamination process cycle causes the analyte to diffuse to the buffer fluid from the sample fluid. A method for separating the analyte is also disclosed.

Abstract: A microfluidic system for separating an analyte from a sample fluid including a series of fluidic channels including at least one first region and at least one second region. The first region includes a plurality of L-nodes, which connects to each other in series. The second region includes a plurality of R-nodes, which connects to each other in series. The first region is configured to trigger at least about one lamination process cycle for both the sample fluid and the buffer fluid and the second region is configured to trigger at least about one reverse lamination process cycle for both the sample fluid and the buffer fluid, whereby the lamination process cycle and the reverse lamination process cycle causes the analyte to diffuse to the buffer fluid from the sample fluid. A method for separating the analyte is also disclosed.

Abstract: This invention relates to apparatus and methods for on-chip monitoring of cellular reactions, and in particular to such an apparatus and method that enables soft-docking of cells by using a dam wall that is parallel to the direction of fluid flow. The invention further relates to an apparatus and method capable of generating a controlled concentration gradient, and in particular a concentration gradient such that the cells are aligned in the direction of the concentration gradient after docking.

Abstract: A tapered microchannel structure allows individual cells to be reacted with a continuum of concentrations or dosages of an analyte or drug from one sample. A dual sandbag structure that divides up a tapered micro channel into thirds permits performing two simultaneous tests on two different sets of cells isolated in the two sandbags by introducing a single analyte or drug into the region between the two sandbag structures.

Abstract: This invention relates to apparatus and methods for on-chip monitoring of cellular reactions, and in particular to such an apparatus and method that enables soft-docking of cells by using a dam wall that is parallel to the direction of fluid flow. The invention further relates to an apparatus and method capable of generating a controlled concentration gradient, and in particular a concentration gradient such that the cells are aligned in the direction of the concentration gradient after docking.