Abstract: A method of proteolyzing a protein, including immobilizing a protein in at least one pore of a porous body, and contacting the protein immobilized in the pore and a protease immobilized on a solid surface such that the protease selectively accesses a site of the protein and proteolyzes the protein at the site.

Abstract: The method according to the present invention for preparing peptide fragments comprises bringing an antibody, which includes a Fc domain immobilized in pores of a porous body, into contact with a protease immobilized on surface of microparticles. Thus, the Fab domain of the antibody is site-specifically cleaved by the protease and a sample containing Fab domain-derived peptide fragments at a high concentration can be obtained. The protease to be used in the present invention is an animal-derived trypsin contaminated with chymotrypsin wherein the chymotrypsin is inactivated and the trypsin is chemically modified at amino group in lysine residues.

Abstract: A method of proteolyzing a protein, including immobilizing a protein in at least one pore of a porous body, and contacting the protein immobilized in the pore and a protease immobilized on a solid surface such that the protease selectively accesses a site of the protein and proteolyzes the protein at the site.

Abstract: A method of proteolyzing a protein, including immobilizing a protein in at least one pore of a porous body, and contacting the protein immobilized in the pore and a protease immobilized on a solid surface such that the protease selectively accesses a site of the protein and proteolyzes the protein at the site.

Abstract: A method of proteolyzing a protein, including immobilizing a protein in at least one pore of a porous body, and contacting the protein immobilized in the pore and a protease immobilized on a solid surface such that the protease selectively accesses a site of the protein and proteolyzes the protein at the site.

Abstract: A method of proteolyzing a protein, including immobilizing a protein in at least one pore of a porous body, and contacting the protein immobilized in the pore and a protease immobilized on a solid surface such that the protease selectively accesses a site of the protein and proteolyzes the protein at the site.

Abstract: A method of proteolyzing a protein, including immobilizing a protein in at least one pore of a porous body, and contacting the protein immobilized in the pore and a protease immobilized on a solid surface such that the protease selectively accesses a site of the protein and proteolyzes the protein at the site.

Abstract: A measurement plate 10 for MALDI mass spectrometry consists of a base plate 11 having an upper surface and a carbon sheet 12 laid on at least a portion of the upper surface. The carbon sheet 12 is a highly-oriented graphite sheet with the orientation direction being parallel to the upper surface. The highly-oriented graphite sheet has a high thermal conductivity in the in-plane direction and a low thermal conductivity in the perpendicular direction. Therefore, the thermal energy generated in a matrix substance by laser irradiation is quickly transferred through the highly-oriented graphite sheet in the in-plane direction. As a result, the thermal energy is uniformly distributed within the laser-irradiated area, so that the reaction of sublimation and ionization of the target sample assisted by the matrix substance uniformly takes place. Thus, a decrease in the detection sensitivity is prevented.