Abstract: The present invention provides a cell culture having a cartilage-like tissue forming property, including a cell population in which the expression intensity of at least one cell surface marker selected from the group consisting of CD166, CD165, CD99, GD2, STRO-1, CD108, CD164, CD6, CD106, and CD107b is not higher than the threshold for each cell surface marker, and/or, the expression intensity of at least one cell surface marker selected from the group consisting of CD26, CD73, CD105, CD44, CD120a, CD201, EGFR, CD146, CD140a, and CD90 is not lower than the threshold for each cell surface marker.

Abstract: Provided is a cell sheet suitable for cartilage repair. The present invention provides a cell sheet for cartilage repair, formed from a culture of cells derived from a cartilage tissue, and the cell sheet is negative for immunostaining using an antibody against type II collagen. The present invention also provides a method for producing a cell sheet for cartilage repair, formed from a culture of cells derived from a cartilage tissue, and the method includes culturing cells derived from a cartilage tissue on a surface of a membrane, where a temperature-responsive polymer is immobilized on the surface, to give the cell sheet. The culturing is stopped before the cell sheet becomes positive for immunostaining using an antibody against type II collagen.

Abstract: Disclosed is a method for isolating or identifying target clone cells from a cell population, the method including steps of: preparing a cell population into which a barcode sequence and at least one reporter protein abnormal expression cassette linked to the barcode sequence are introduced; introducing a barcode sequence recognition module targeting an arbitrary barcode sequence and a nucleic acid mutation repair enzyme into cells; repairing a nucleic acid mutation causing abnormal expression occurring in the at least one reporter protein abnormal expression cassette by expression of a complex of the barcode sequence recognition module and the nucleic acid mutation repair enzyme in a cell containing the target barcode sequence, to induce normal expression of the reporter protein; and isolating or identifying target clone cells in which the reporter protein is expressed.

Abstract: A cell culture device comprises a first inner cylinder formed in a tubular shape, a cell culture membrane placed to cover over an entire one end of the first inner cylinder and configured to be folded down toward an outer surface of the first inner cylinder along an outer circumference at the one end of the first inner cylinder, and an outer cylinder formed in a tubular shape, configured to place the first inner cylinder inside thereof such that a height direction of the outer cylinder is identical with a height direction of the first inner cylinder, and provided with a first slit in an area overlapping with at least the one end of the first inner cylinder to be extended in the height direction, wherein the cell culture membrane is held between an inner wall surface of the outer cylinder and the outer surface of the first inner cylinder.

Abstract: The present invention relates to a method for producing a double-stranded DNA fragment having a desired nucleotide sequence through dual asymmetric PCR (DA-PCR). The method comprises: (1) providing a plurality of oligonucleotides (sense oligonucleotides) each corresponding to a part of a sense strand of the double-stranded DNA fragment and a plurality of oligonucleotides (antisense oligonucleotides) each corresponding to a part of an antisense strand of the double-stranded DNA fragment and mixing together the oligonucleotides with equal concentrations, DNA polymerase, and dNTP to prepare a reaction mixture solution; (2) performing PCR by using the reaction mixture solution from step (1); (3) adding a primer set capable of amplifying the double-stranded DNA fragment of full length to the reaction mixture solution from step (2); and (4) performing PCR by using the reaction mixture solution from step (3).

Abstract: Provided are a stent to be placed in the bile duct and a process for producing the stent, the stent hollow being less apt to be blocked even when the stent is placed in the bile duct for a long period. The inner peripheral surface of the stent is coated with a resin layer with resistance to sludge formation that includes a polymer obtained by polymerizing 2-methoxyethyl acrylate. This stent is produced by applying a coating fluid that contains 0.1-0.5 mass % polymer obtained by polymerizing 2-methoxyethyl acrylate, to the inner peripheral surface of a stent.

Abstract: Provided is a cell sheet suitable for cartilage repair. The present invention provides a cell sheet for cartilage repair, formed from a culture of cells derived from a cartilage tissue, and the cell sheet is negative for immunostaining using an antibody against type II collagen. The present invention also provides a method for producing a cell sheet for cartilage repair, formed from a culture of cells derived from a cartilage tissue, and the method includes culturing cells derived from a cartilage tissue on a surface of a membrane, where a temperature-responsive polymer is immobilized on the surface, to give the cell sheet. The culturing is stopped before the cell sheet becomes positive for immunostaining using an antibody against type II collagen.

Abstract: Provided is a polymer material having effective antibacterial properties and having bio adaptability in which cytotoxicity, especially low hemolysis, when used in contact with in vivo tissue and blood is suppressed so as to be low. The polymer is characterized by comprising a main chain and a side chain portion which is linked to the main chain via a linker and which contains at least a structure specified by the following (A) and (B). (A) A structure containing a cationic group and (B) a structure in which expression of bio adaptability is expected.

Abstract: A cell culture device comprises a first inner cylinder formed in a tubular shape. a cell culture membrane placed to cover over an entire one end of the first inner cylinder and configured to be folded down toward an outer surface of the first inner cylinder along an outer circumference at the one end of the first inner cylinder, and an outer cylinder formed in a tubular shape, configured to place the first inner cylinder inside thereof such that a height direction of the outer cylinder is identical with a height direction of the first inner cylinder, and provided with a first slit in an area overlapping with at least the one end of the first inner cylinder to be extended in the height direction, wherein the cell culture membrane is held between an inner wall surface of the outer cylinder and the outer surface of the first inner cylinder.

Abstract: Provided are a stent to be placed in the bile duct and a process for producing the stent, the stent hollow being less apt to be blocked even when the stent is placed in the bile duct for a long period. The inner peripheral surface of the stent is coated with a resin layer with resistance to sludge formation that includes a polymer obtained by polymerizing 2-methoxyethyl acrylate. This stent is produced by applying a coating fluid that contains 0.1-0.5 mass % polymer obtained by polymerizing 2-methoxyethyl acrylate, to the inner peripheral surface of a stent.

Abstract: A bird knocking-off rod of a bird knocking-off device is disposed outside a jet engine, in front of an air inlet of the jet engine and capable of rotating with a rotation area that is larger than an opening area of the air inlet of the jet engine. The rotary drive unit contains a rotary drive source and is capable of rotationally driving the bird knocking-off rod independently of rotation of the jet engine. Thus, bird strike against aircraft can be prevented.

Abstract: A display control apparatus controls attributes of characters to be displayed on a display unit. The display control apparatus includes a storage storing predetermined attributes, and a converter for converting attributes of each of characters making up an input character string by referring to the storage so that characters making up a converted character string have the predetermined attribute. The converted character string is output for display on the display unit.

Abstract: A display control apparatus controls attributes of characters to be displayed on a display unit. The display control apparatus includes a storage storing predetermined attributes, and a converter for converting attributes of each of characters making up an input character string by referring to the storage so that characters making up a converted character string have the predetermined attribute. The converted character string is output for display on the display unit.