Abstract: A suture method includes a first arrangement step of using a first treatment device including first/second grasping pieces to hook the first grasping piece to a first position of peripheral tissues of a lesion; a second arrangement step of hooking the second grasping piece to a second position of the peripheral tissues; a grasping step of grasping the first position and the second position while pressing the first position and the second position to the peripheral tissues; a retracting step of retracting the lesion site toward a proximal end side of the first treatment device while drawing the first position and the second position grasped by the first treatment device; and a suturing step of suturing a first suturing position at an external side of the first position and a second suturing position at an external side of the second position in the peripheral tissues.

Abstract: A cell cluster from which unwanted components are removed by efficiently washing the cell cluster is collected. Provided is a cell washing method including a centrifuging step (S1) of collecting a cell suspension in a centrifuge container having a washing fluid discharge port opposing a bottom part and centrifugally separating cells at the bottom part by rotating in such a manner that the bottom part faces outward in the radial direction; a supernatant ejecting step (S2) of ejecting a supernatant centrifugally separated in the centrifuging step (S1); and a washing step (S3) of discharging the washing fluid from the discharge port while rotating the centrifuge container while ejecting the supernatant in the supernatant ejecting step (S2).

Abstract: To enable to yield a group of fat-derived stem cells of constant quality regardless of the individual difference in fat tissue.