Abstract: The present invention provides an optimization control method for an aero-engine transient state based on reinforcement learning, and belongs to the technical field of aero-engine transient states. The method comprises: adjusting an existing twin-spool turbo-fan engine model as a model for invoking a reinforcement learning algorithm; to simultaneously satisfy high level state space and continuous action output of a real-time model, designing an Actor-Critic network model; designing a deep deterministic policy gradient (DDPG) algorithm based on an Actor-Critic frame, to simultaneously solve the problems of high-dimensional state space and continuous action output; training the model after combining the Actor-Critic frame with the DDPG algorithm; and obtaining the control law of engine acceleration transition from the above training process, and using the method to control an engine acceleration process.

Abstract: Methods are provided for nucleic acid amplification including contacting a double stranded nucleic acid with transposases bound to transposon DNA, wherein the transposon DNA includes a transposase binding site and an RNA polymerase promoter sequence, wherein the transposases/transposon DNA complex bind to target locations along the double stranded nucleic acid and cleave the double stranded nucleic acid into a plurality of double stranded fragments, with each double stranded fragment having the transposon DNA bound to each 5? end of the double stranded fragment, extending the double stranded fragments along the transposon DNA to make double stranded extension products having double stranded RNA polymerase promoter sequences at each end, contacting the double stranded extension products with an RNA polymerase to make a plurality of RNA transcripts of each double stranded extension product, reverse transcribing the RNA transcripts into single stranded copy DNA, forming complementary strands to the single strand

Abstract: Methods are provided for nucleic acid amplification including contacting a double stranded nucleic acid with transposases bound to transposon DNA, wherein the transposon DNA includes a transposase binding site and an RNA polymerase promoter sequence, wherein the transposases/transposon DNA complex bind to target locations along the double stranded nucleic acid and cleave the double stranded nucleic acid into a plurality of double stranded fragments, with each double stranded fragment having the transposon DNA bound to each 5? end of the double stranded fragment, extending the double stranded fragments along the transposon DNA to make double stranded extension products having double stranded RNA polymerase promoter sequences at each end, contacting the double stranded extension products with an RNA polymerase to make a plurality of RNA transcripts of each double stranded extension product, reverse transcribing the RNA transcripts into single stranded copy DNA, forming complementary strands to the single strand