Abstract: Methods are provided herein for assembling at least two nucleic acids using a sequence specific nuclease agent (e.g., a gRNA-Cas complex) to create end sequences having complementarity and subsequently assembling the overlapping complementary sequences. The nuclease agent (e.g., a gRNA-Cas complex) can create double strand breaks in dsDNA in order to create overlapping end sequences or can create nicks on each strand to produce complementary overhanging end sequences. Assembly using the method described herein can assemble any nucleic acids having overlapping sequences or can use a joiner oligo to assemble sequences without complementary ends.

Abstract: An apparatus and method for sampling and reporting performance of a communication network includes a first step 200 of defining reporting probability-based rules for sampling and reporting network performance measurements. A next step 202 includes providing the rules to a plurality of mobile stations attached to the network. A next step 204 includes sampling network measurements by the mobile stations in accordance with the rules. A next step 206 includes reporting the network performance measurements to the network in accordance with the rules.

Abstract: A system and method for physical cell identification in a wireless communication system includes a first step 500 of providing a set of temporary physical cell identifications for cells. A next step 502 includes identifying a new cell in the communication system. A next step 506 includes allocating a temporary physical cell identification for the new cell. A next step 510 includes changing the temporary physical cell identification of the new cell to a permanent physical cell identification.