Abstract: A mass spectrometry method comprises: providing a multiplexed sample comprising a mixture of biomolecule-containing samples respectively tagged with mass tags; acquiring MS2 spectra by data-dependent acquisition (DDA) of the multiplexed sample or another mass tagged mixture of the samples during chromatographic elution; acquiring MS2 spectra by data-independent acquisition (DIA) during the elution; forming a spectral library from the DDA MS2 spectra comprising a plurality of the MS2 spectra and the biomolecule retention times; matching fragment-ion peaks in the DIA MS2 spectra to fragment-ion peaks in the MS2 library spectra to find matched biomolecules; determining a total abundance for each matched biomolecule from the DIA MS2 spectra at each of a plurality of retention times; determining abundances of respective reporter ions from the DIA MS2 spectra at the plurality of retention times; and deconvoluting relative abundances of the biomolecules in each respectively tagged biomolecule-containing sample based

Abstract: A hood type cover (4) with lifting mechanism is engaged over a crucible (1) with several material pockets (2, 3). By means of the lifting mechanism, the distance between a surface (5) of the crucible and a seal (8) on the cover (4) is varied. The lifting mechanism comprises a track (12) disposed on the wall (16) of the crucible (1). Engaged in this track are guided elements which are connected to the cover and are forcibly guided and moved upon rotary movements of the crucible (1). These guiding elements bring about a pivoting of the cover (4) or a displacement of the cover (4) or of the crucible (1) parallel to the central axis (17) of the crucible (1). This arrangement of the cover (4) permits a safe positioning of the material pockets (2, 3) inside the vacuum chamber of a coating installation.