Abstract: Provided are methods, systems and modules useful in split and pool workflow.

Abstract: The present invention relates to expression cassettes comprising at least one transcription regulating nucleotide sequence obtainable from the group of genes of monocotyledonous plants consisting of caffeoyl-CoA-O-methyltransferase genes, C8,7-sterol isomerase genes, hydroxyproline-rich glycoprotein (HRGP) genes, lactate dehydrogenase genes, and chloroplast protein 12 like genes. More preferably the transcription regulating sequences are obtainable from Zea mays or Oryza sativa. The transcription regulating sequences are especially useful for root/kernel-preferential, leaf/endosperm-preferential, root/silk/kernel-preferential, or constitutive expression.

Abstract: The present invention relates to expression cassettes comprising at least one transcription regulating nucleotide sequence obtainable from the group of genes of monocotyle-donous plants consisting of caffeoyl-CoA-O-methyltransferase genes, C8,7-sterol isomerase genes, hydroxyproline-rich glycoprotein (HRGP) genes, lactate dehydrogenase genes, and chloroplast protein 12 like genes. More preferably the transcription regulating sequences are obtainable from Zea mays or Oryza sativa. The transcription regulating sequences are especially useful for root/kernel-preferential, leaf/endosperm-preferential, root/silk/kernel-preferential, or constitutive expression.

Abstract: The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.

Abstract: The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.

Abstract: The present invention relates generally to the field of molecular biology and concerns a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a GRP (Growth-Related Protein). The present invention also concerns plants having modulated expression of a nucleic acid encoding a GRP, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. The GRP may be one of the following: Seed Yield Regulator (SYR), FG-GAP, CYP90B, CDC27, AT-hook transcription factors, DOF transcription factors and Cyclin Dependent Kinase Inhibitors (CKIs).

Abstract: The present invention relates generally to the field of molecular biology and concerns a method for increasing plant yield relative to suitable control plants. More specifically, the present invention concerns a method for increasing plant yield comprising increasing expression in a plant of a nucleic acid encoding a Dof (DNA-binding with one finger) domain transcription factor polypeptide. The present invention also concerns plants having increased expression of a nucleic acid encoding a Dof domain transcription factor polypeptide, which plants have increased yield relative to suitable control plants. The invention also provides constructs useful in the methods of the invention.

Abstract: The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics. More specifically, the present invention concerns a method for improving plant growth characteristics by modulating expression in a plant of a plant nucleic acid encoding a cyclin dependent kinase (CDK) and/or by modulating activity in a plant of a plant CDK protein, which CDK protein comprises different motifs or which CDK nucleic acid encodes such protein. The present invention also concerns plants having modulated expression of a plant CDK nucleic acid and/or modulated activity of a plant CDK protein, which CDK protein comprises different sequence motifs or which nucleic acid encodes such protein and which plants have improved growth characteristics relative to corresponding wild type plants.

Abstract: A method for producing a plant with increased yield as compared to a corresponding wild type plant whereby the method comprises at least the following step: increasing or generating in a plant or a part thereof one or more activities of a polypeptide selected from the group consisting of 2-oxoglutarate-dependent dioxygenase, 3-ketoacyl-CoA thiolase, 3?-phosphoadenosine 5?-phosphate phosphatase, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, 5OS chloroplast ribosomal protein L21, 57972199. R01.1-protein, 60952769. R01.1-protein, 60S ribosomal protein, ABC transporter family protein, AP2 domain-containing transcription factor, argonaute protein, AT1 G29250.

Abstract: The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.

Abstract: The present invention relates to the field of agricultural biotechnology. Disclosed herein are expression constructs with expression specificity for the starchy endosperm and/or the germinating embryo, transgenic plants comprising such expression constructs, and methods of making and using such DNA constructs and transgenic plants.

Abstract: The present invention relates to the field of agricultural biotechnology. Disclosed herein are methods, nucleic acid molecules and expression constructs or vectors thereof for an expression specific for the germinating embryo, transgenic plants and cells comprising such nucleic acids, vectors, expression constructs, as well as methods of making and using such DNA constructs and transgenic plant.

Abstract: The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics. More specifically, the present invention concerns a method for improving plant growth characteristics by modulating expression in a plant of a plant nucleic acid encoding a cyclin dependent kinase (CDK) and/or by modulating activity in a plant of a plant CDK protein, which CDK protein comprises different motifs or which CDK nucleic acid encodes such protein. The present invention also concerns plants having modulated expression of a plant CDK nucleic acid and/or modulated activity of a plant CDK protein, which CDK protein comprises different sequence motifs or which nucleic acid encodes such protein and which plants have improved growth characteristics relative to corresponding wild type plants.

Abstract: The invention relates to methods for the identification and use of introns with gene expression enhancing properties. The teaching of this invention enables the identification of introns causing intron-mediated enhancement (IME) of gene expression. The invention furthermore relates to recombinant expression construct and vectors comprising said IME-introns operably linked with a promoter sequence and a nucleic acid sequence. The present invention also relates to transgenic plants and plant cells transformed with these recombinant expression constructs or vectors, to cultures, parts or propagation material derived there from, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.

Abstract: The present invention relates to the field of agricultural biotechnology. Disclosed herein are expression constructs with expression specificity for the starchy endosperm and/or the germinating embryo, transgenic plants comprising such expression constructs, and methods of making and using such DNA constructs and transgenic plants.

Abstract: The present invention relates generally to the field of molecular biology and concerns a method for increasing plant yield relative to suitable control plants plants. More specifically, the present invention concerns a method for increasing plant yield comprising increasing expression in a plant of a nucleic acid encoding a Dof (DNA-binding with one finger) domain transcription factor polypeptide. The present invention also concerns plants having increased expression of a nucleic acid encoding a Dof domain transcription factor polypeptide, which plants have increased yield relative to suitable control plants. The invention also provides constructs useful in the methods of the invention.

Abstract: The present invention relates to expression cassettes comprising at least one transcription regulating nucleotide sequence obtainable from the group of genes of monocotyle-donous plants consisting of caffeoyl-CoA-O-methyltransferase genes, C8,7-sterol isomerase genes, hydroxyproline-rich glycoprotein (HRGP) genes, lactate dehydrogenase genes, and chloroplast protein 12 like genes. More preferably the transcription regulating sequences are obtainable from Zea mays or Oryza sativa. The transcription regulating sequences are especially useful for root/kernel-preferential, leaf/endosperm-preferential, root/silk/kernel-preferential, or constitutive expression.

Abstract: The invention relates to transgenic expression constructs and vectors comprising plant promoters with a non-seed tissue, preferably vegetative plant tissue specific expression profile, and the use of these transgenic expression constructs or vectors for the transgenic expression of nucleic acid sequences in plants. More preferably, the promoters of the invention are the promoter of the Pisum sativum ptxA gene, or the promoter of the Glycine max extensin (SbHRGP3) gene, and functional equivalent fragments and functional equivalent homologs thereof, or their complements, having essentially the same promoter activity. The invention furthermore relates to transgenic plants and plant cells transformed with these expression constructs or vectors, to cultures, parts or propagation material derived therefrom, and to the use of same for the preparation of foodstuffs, animal feeds, seed, pharmaceuticals or fine chemicals, to improve plant biomass, yield, or provide desirable phenotypes.