Abstract: Compositions and methods for gene editing. In some embodiments, a polynucleotide encoding Cas9 is provided that can provide one or more of improved editing efficiency, reduced immunogenicity, or other benefits.

Abstract: Polynucleotides, polypeptides, compositions, and methods for genome editing using deamination are provided. An mRNA containing an open reading frame (ORF) encoding a polypeptide is provided herein. The polypeptide includes a cytidine deaminase and an RNA-guided nickase, and does not include a uracil glycosylase inhibitor (UGI). A composition provided herein may include two different mRNAs. The first mRNA includes an ORF encoding a cytidine deaminase and an RNA-guided nickase, and the second mRNA includes an ORF encoding uracil glycosylase inhibitor (UGI).

Abstract: This disclosure relates to the field of poly-adenylated (poly-A) tails. In some embodiments, a DNA encodes a poly-A tail located 3? to nucleotides encoding a protein of interest, wherein the poly-A tail comprises one or more non-adenine nucleotide.

Abstract: Compositions and methods for gene editing. In some embodiments, a polynucleotide encoding Cas9 is provided that can provide one or more of improved editing efficiency, reduced immunogenicity, or other benefits.

Abstract: This disclosure relates to the field of poly-adenylated (poly-A) tails. In some embodiments, a DNA encodes a poly-A tail located 3? to nucleotides encoding a protein of interest, wherein the poly-A tail comprises one or more non-adenine nucleotide.

Abstract: Compositions and methods for gene editing. In some embodiments, a polynucleotide encoding Cas9 is provided that can provide one or more of improved editing efficiency, reduced immunogenicity, or other benefits.

Abstract: An embodiment of the invention relates to a method of operating a communication system that comprises a master node (MN) and at least two slave nodes (SN1-SN4) and is operated in a time-division multiplexing technique. A transmission subframe (TSF) comprises at least one downlink slot (DS) for transmission of downlink data packets (DDP) from the master node (MN) to the slave nodes (SN1-SN4), and at least one uplink slot (US1-US4) for transmission of uplink data packets (UDP) from each of the slave nodes (SN1-SN4) to the master node (MN). The master node (MN) evaluates the uplink data packets (UDP) and generates a re-transmission schedule (RTS) that defines a re-transmission of uplink and/or downlink data packets (UDP, DDP) in the auxiliary subframe (ASF). Prior to the re-transmission of uplink and/or downlink data packets (UDP, DDP) in the auxiliary subframe (ASF), the master node (MN) broadcasts the re-transmission schedule (RTS) to the slave nodes (SN1-SN4).

Abstract: Compositions and methods for gene editing. In some embodiments, a polynucleotide encoding Cas9 is provided that can provide one or more of improved editing efficiency, reduced immunogenicity, or other benefits.

Abstract: This disclosure relates to the field of poly-adenylated (poly-A) tails. In some embodiments, a DNA encodes a poly-A tail located 3? to nucleotides encoding a protein of interest, wherein the poly-A tail comprises one or more non-adenine nucleotide.

Abstract: The invention provides lipid nanoparticle-based compositions and methods useful for delivery of CRISPR/Cas gene editing components.

Abstract: Heparan sulphate HS/BMP2 is disclosed, together with the use of HS/BMP2 in the repair and regeneration of bone tissue.

Abstract: Heparan sulphate HS/BMP2 is disclosed, together with the use of HS/BMP2 in the repair and regeneration of bone tissue.

Abstract: The isolation and identification of glycosaminoglycans capable of binding to proteins having a heparin-binding domain is disclosed, as well as the use of the glycosaminoglycans isolated in the growth and/or development of tissue.

Abstract: The invention relates to heparan sulphate GAGs obtained by affinity chromatography using the heparin-binding domain of BMP2. The GAGs were obtained from osteoblast extracellular matrix and from a commercially available heparan sulfate (Celsus HS).

Abstract: Compositions and methods are provided for enhancing the efficiency of gene editing by timing the expression and activity of a nuclease to correspond with availability of a repair template. Compositions and methods for temporally regulating the duration of nuclease activity, and methods of selectively preventing nuclease expression during viral vector production, are also provided.

Abstract: Compositions and methods are provided for enhancing the efficiency of gene editing by timing the expression and activity of a nuclease to correspond with availability of a repair template. Compositions and methods for temporally regulating the duration of nuclease activity, and methods of selectively preventing nuclease expression during viral vector production, are also provided.

Abstract: Heparan sulphate HS/BMP2 is disclosed, together with the use of HS/BMP2 in the repair and regeneration of bone tissue.

Abstract: Heparan sulphate HS/BMP2 is disclosed, together with the use of HS/BMP2 in the repair and regeneration of bone tissue.

Abstract: Heparan sulphate HS/BMP2 is disclosed, together with the use of HS/BMP2 in the repair and regeneration of bone tissue.

Abstract: The invention discloses a method for sending real-time sensitive data using a transmitter for a multi channel communication system, wherein the communication system has n sub-channels, wherein n is greater than 1, wherein the sub-channels are spatially different and/or the sub-channels have different frequencies. The method has the steps of receiving information about the channel state of the n sub-channels, receiving information about the number of statistically independent sub-channels m, wherein m is less than or equal to n, and receiving data intended to be sent.