Patents by Inventor Christian Eggeling
Christian Eggeling has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9759659Abstract: A method for identifying the impact on data, such as experimental data, of interfering effects, such as unwanted auto-fluorescence, fluorescence quenching, and fluorescent-sample deterioration, whether or not the data fulfill certain criteria with respect to a threshold indicative of the interfering effects.Type: GrantFiled: March 10, 2011Date of Patent: September 12, 2017Assignee: EVOTEC AGInventors: Christian Eggeling, Kaupo Palo, Nicolas Fay, Maciej Hoffman-Wecker, Pierre Ilouga
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Patent number: 9551658Abstract: In a STED fluorescence light microscope pulses of excitation light (3) are applied to a sample, which excite fluorescent entities contained in the sample for fluorescence, and which are focused on at least one focal area. Further, de-excitation light (12) is applied to the sample, which de-excites the excited fluorescent entities and which comprises an intensity zero point in the at least one focal area, as a continuous wave. Fluorescence light emitted by the excited fluorescent entities in the sample is registered after each pulse of the excitation light (3) and overlapping with applying the de-excitation light (13) with high temporal resolution between consecutive pulses of the excitation light (3).Type: GrantFiled: May 22, 2013Date of Patent: January 24, 2017Assignees: MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V., DEUTSCHES KREBSFORSCHUNGSZENTRUMInventors: Stefan W. Hell, Johann Engelhardt, Matthias Reuss, Volker Westphal, Christian Eggeling, Gael Moneron, Kyu-Young Han, Giuseppe Vicidomini, Katrin Willig
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Patent number: 8735444Abstract: The present invention relates to novel fluorinated 3,6-diaminoxanthene compounds derived from the basic structural formula (I) and to their uses as photostable fluorescent dyes, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional microscopy, stimulated emission depletion (STED) reversible saturable optically linear fluorescent transitions (RESOLFT) microscopy, and fluorescence correlation spectroscopy. The claimed compounds are also useful as molecular probes in various spectroscopic applications.Type: GrantFiled: June 26, 2009Date of Patent: May 27, 2014Assignee: Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.Inventors: Stefan W. Hell, Vladimir N. Belov, Gyuzel Mitronova, Mariano Bossi, Gael Moneron, Christian A. Wurm, Stefan Jakobs, Christian Eggeling, Jakob Bierwagen, Lars Meyer
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Patent number: 8580579Abstract: The invention relates to novel and improved photostable rhodamine dyes of the general structural formulae I or II and their uses as fluorescent markers, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional and stimulated emission depletion (STED) microscopy and fluorescence correlation spectroscopy.Type: GrantFiled: April 26, 2010Date of Patent: November 12, 2013Assignee: Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.Inventors: Stefan Hell, Vladimir N. Belov, Kirill Kolmakov, Volker Westphal, Marcel Lauterbach, Stefan Jakobs, Christian Wurm, Christian Eggeling, Christian Ringemann
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Publication number: 20130256564Abstract: In a STED fluorescence light microscope pulses of excitation light (3) are applied to a sample, which excite fluorescent entities contained in the sample for fluorescence, and which are focused on at least one focal area. Further, de-excitation light (12) is applied to the sample, which de-excites the excited fluorescent entities and which comprises an intensity zero point in the at least one focal area, as a continuous wave. Fluorescence light emitted by the excited fluorescent entities in the sample is registered after each pulse of the excitation light (3) and overlapping with applying the de-excitation light (13) with high temporal resolution between consecutive pulses of the excitation light (3).Type: ApplicationFiled: May 22, 2013Publication date: October 3, 2013Inventors: Stefan W. Hell, Johann Engelhardt, Matthias Reuss, Volker Westphal, Christian Eggeling, Gael Moneron, Kyu-Young Han, Giuseppe Vicidomini, Katrin Willig
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Publication number: 20120135459Abstract: The present invention relates to novel fluorinated 3,6-diaminoxanthene compounds derived from the basic structural formula (I) and to their uses as photostable fluorescent dyes, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional microscopy, stimulated emission depletion (STED) reversible saturable optically linear fluorescent transitions (RESOLFT) microscopy, and fluorescence correlation spectroscopy. The claimed compounds are also useful as molecular probes in various spectroscopic applications.Type: ApplicationFiled: June 26, 2009Publication date: May 31, 2012Applicant: Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.Inventors: Stefan W. Hell, Vladimir N. Belov, Gyuzel Mitronova, Mariano Bossi, Gael Moneron, Christian A. Wurm, Stefan Jakobs, Christian Eggeling, Jakob Bierwagen, Lars Meyer
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Patent number: 8174692Abstract: In high spatial resolution imaging, a structure in a specimen is marked with a substance which, in a first electronic state, is excited by light of one wavelength to emit fluorescent light, which is also converted from its first into a second electronic state by that light, and which returns from its second into its first electronic state. The specimen is imaged onto a sensor at a spatial resolution not resolving an average spacing between neighboring molecules of the substance, and exposed to the light at such an intensity that the molecules in the first state are alternately excited to emit fluorescent light and converted into their second state, and that at least 10% of the molecules presently in their first state lie at a distance from their closest neighboring molecules in their first state which is greater than the spatial resolution of the imaging onto the sensor.Type: GrantFiled: November 18, 2010Date of Patent: May 8, 2012Assignee: Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.Inventors: Stefan W. Hell, Jonas Fölling, Christian Eggeling, Alexander Egner, Andreas Schönle, Mariano Bossi
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Publication number: 20120100559Abstract: The invention relates to novel and improved photostable rhodamine dyes of the general structural formulae I or II and their uses as fluorescent markers, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional and stimulated emission depletion (STED) microscopy and fluorescence correlation spectroscopy. The partially deuterated analogues are useful as molecular mass distribution tags in mass spectroscopic applications.Type: ApplicationFiled: April 26, 2010Publication date: April 26, 2012Applicant: Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.Inventors: Stefan Hell, Vladimir N. Belov, Kirill Kolmakov, Volker Westphal, Marcel Lauterbach, Stefan Jakobs, Christian Wurm, Christian Eggeling, Christian Ringemann
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Patent number: 8084754Abstract: For imaging of a structure, the structure is marked with a substance which can be converted by a switching signal from a first into a second state, and which provides an optical measurement signal in one of its states, only. The switching signal is applied such that at least 10% of the molecules of the substance being in the measurement signal providing state are at a distance from their closest neighbors, which is greater than the spatial resolution limit of imaging the specimen onto a sensor array, which in turn is greater than an average distance between the molecules of the substance. From an intensity distribution of the measurement signal recorded with the sensor array, the position is only determined for those molecules of the substance which are at a distance from their closest neighboring molecules in the measurement signal providing state, which is greater than the spatial resolution limit.Type: GrantFiled: January 25, 2011Date of Patent: December 27, 2011Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften E.V.Inventors: Stefan Hell, Christian Eggeling, Alexander Egner, Jonas Fölling, Andreas Schönle, Mariano Bossi
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Publication number: 20110218767Abstract: The invention provides a method for identifying the impacts of interfering effects on experimental data. In particular, a method is described for identifying the impacts of unwanted auto-fluorescence, fluorescence quenching, and deterioration of a fluorescent sample under study on the collected experimental data. The data are analyzed whether or not said data fulfill certain criteria with respect to a threshold which is indicative for said interfering effect.Type: ApplicationFiled: March 10, 2011Publication date: September 8, 2011Inventors: Christian Eggeling, Kaupo Palo, Nicolas Fay, Maciej Hoffman-Wecker, Pierre Ilouga
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Publication number: 20110160083Abstract: For imaging of a structure, the structure is marked with a substance which can be converted by a switching signal from a first into a second state, and which provides an optical measurement signal in one of its states, only. The switching signal is applied such that at least 10% of the molecules of the substance being in the measurement signal providing state are at a distance from their closest neighbors, which is greater than the spatial resolution limit of imaging the specimen onto a sensor array, which in turn is greater than an average distance between the molecules of the substance. From an intensity distribution of the measurement signal recorded with the sensor array, the position is only determined for those molecules of the substance which are at a distance from their closest neighboring molecules in the measurement signal providing state, which is greater than the spatial resolution limit.Type: ApplicationFiled: January 25, 2011Publication date: June 30, 2011Inventors: Stefan Hell, Christian Eggeling, Alexander Egner, Jonas Fölling, Andreas Schönle, Mariano Bossi
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Publication number: 20110081653Abstract: In high spatial resolution imaging, a structure in a specimen is marked with a substance which, in a first electronic state, is excited by light of one wavelength to emit fluorescent light, which is also converted from its first into a second electronic state by that light, and which returns from its second into its first electronic state. The specimen is imaged onto a sensor at a spatial resolution not resolving an average spacing between neighboring molecules of the substance, and exposed to the light at such an intensity that the molecules in the first state are alternately excited to emit fluorescent light and converted into their second state, and that at least 10% of the molecules presently in their first state lie at a distance from their closest neighboring molecules in their first state which is greater than the spatial resolution of the imaging onto the sensor.Type: ApplicationFiled: November 18, 2010Publication date: April 7, 2011Applicant: Max-Planck-Gesellschaft zur Forderung der Wissenschaftern e.V.Inventors: Stefan W. HELL, Jonas FÖLLING, Christian EGGELING, Alexander EGNER, Andreas SCHÖNLE, Mariano BOSSI
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Patent number: 7880150Abstract: For the high spatial resolution imaging of a structure of interest in a specimen, a substance is selected from a group of substances which have a fluorescent first state and a nonfluorescent second state; which can be converted fractionally from their first state into their second state by light which excites them into fluorescence, and which return from their second state into their first state; the specimen's structure of interest is imaged onto a sensor array, a spatial resolution limit of the imaging being greater (i.e.Type: GrantFiled: May 29, 2008Date of Patent: February 1, 2011Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.Inventors: Stefan Hell, Christian Eggeling, Alexander Egner, Jonas Fölling, Andreas Schönle, Mariano Bossi
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Publication number: 20100211328Abstract: The invention provides a method for identifying the impacts of interfering effects on experimental data. In particular, a method is described for identifying the impacts of unwanted auto-fluorescence, fluorescence quenching, and deterioration of a fluorescent sample under study on the collected experimental data. The data are analyzed whether or not said data fulfill certain criteria with respect to a threshold which is indicative for said interfering effect.Type: ApplicationFiled: January 29, 2010Publication date: August 19, 2010Inventors: Christian Eggeling, Kaupo Palo, Nicolas Fay, Maciej Hoffman-Wecker, Pierre Ilouga
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Publication number: 20100140506Abstract: A method of determining a measurement value on the basis of a plurality of single molecule events of marker molecules in a sample comprises the steps of selecting the marker molecules from a group of marker molecules which are transferable between a measurable state in which a measurement signal necessary for determining the at least one measurement value is obtainable from the marker molecules and a non-measurable state, of providing the marker molecules in the sample at such an absolute concentration that the at least one measurement value is not determinable, if all marker molecules are in their measurable state, and adjusting a measurement concentration of the marker molecules in the measurable state by means of applying the physical signal to the sample at such an intensity that the at least one measurement value is determinable within a defined measurement area of the sample.Type: ApplicationFiled: January 15, 2010Publication date: June 10, 2010Applicant: Max-Planck-Gesellschaft zur Forderung der wissenschaften e.V.Inventors: Christian EGGELING, Andreas SCHÖNLE, Stefan W. HELL, Michael HILBERT
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Patent number: 7719679Abstract: The inventive method for optically measuring a sample consists in temporarily repeatedly transmitting an electromagnetic signal (2) to the sample in such a way that a substance contained in the sample is transferred from a first electronic state (1) into a second electronic state (3), wherein at least one part of said substance in the second state (3) emits photons which are used for carrying out the optical measurement of the sample, the signal (2) is transmitted to the same sample area at a certain repetition interval and said repetition interval of the signal (2) is adjusted with a lifetime of the second state (3) of the substance having an order of magnitude of 1 ns on a value of at least 0.1 ?s which is optimized with respect to photon yield from the substance.Type: GrantFiled: December 13, 2007Date of Patent: May 18, 2010Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.Inventors: Stefan Hell, Christian Eggeling, Gerald Donnert
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Publication number: 20090134342Abstract: For the high spatial resolution imaging of a structure of interest in a specimen, a substance is selected from a group of substances which have a fluorescent first state and a nonfluorescent second state; which can be converted fractionally from their first state into their second state by light which excites them into fluorescence, and which return from their second state into their first state; the specimen's structure of interest is imaged onto a sensor array, a spatial resolution limit of the imaging being greater (i.e.Type: ApplicationFiled: May 29, 2008Publication date: May 28, 2009Inventors: Stefan Hell, Christian Eggeling, Alexander Egner, Jonas Folling, Andreas Schonle, Mariano Bossi
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Publication number: 20080270073Abstract: The invention provides a method for identifying the impacts of interfering effects on experimental data. In particular, a method is described for identifying the impacts of unwanted auto-fluorescence, fluorescence quenching, and deterioration of a fluorescent sample under study on the collected experimental data. The data are analyzed whether or not said data fulfill certain criteria with respect to a threshold which is indicative for said interfering effect.Type: ApplicationFiled: February 8, 2008Publication date: October 30, 2008Inventors: Christian Eggeling, Kaupo Palo, Nicolas Fay, Maciej Hoffman-Wecker, Pierre Ilouga
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Publication number: 20080088839Abstract: The inventive method for optically measuring a sample consists in temporarily repeatedly transmitting an electromagnetic signal (2) to the sample in such a way that a substance contained in the sample is transferred from a first electronic state (1) into a second electronic state (3), wherein at least one part of said substance in the second state (3) emits photons which are used for carrying out the optical measurement of the sample, the signal (2) is transmitted to the same sample area at a certain repetition interval and said repetition interval of the signal (2) is adjusted with a lifetime of the second state (3) of the substance having an order of magnitude of 1 ns on a value of at least 0.1 ?s which is optimized with respect to photon yield from the substance.Type: ApplicationFiled: December 13, 2007Publication date: April 17, 2008Applicant: Max Planck-Gesellschaft zur Forderung der Wissenschaften e.V.Inventors: Stefan Hell, Christian Eggeling, Gerald Donnert
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Patent number: 7345759Abstract: A device for the measurement of chemical and/or biological samples, in particular by means of luminescence spectroscopy, comprises an irradiation unit, a sample receiver, at least one optical unit and a detector unit. The color marker in the sample, which contains at least one color marker, is stimulated by irradiation into producing luminescence and gives off light. The light emitted by the color markers is detected by detectors in the detector unit. According to the invention, the measurement results may be improved by the irradiation unit generating pulsed irradiation. The irradiation unit is thus preferably controlled by a control unit in such a way that the irradiation pulses impinge on the sample in a temporal sequence.Type: GrantFiled: August 22, 2001Date of Patent: March 18, 2008Assignee: Evotec OAI AGInventors: Rolf Guenther, Leif Brand, Christian Eggeling, Karsten Gall, Claus Seidel