Patents by Inventor Christian Korfhage
Christian Korfhage has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240158836Abstract: The technology provided herein relates to high resolution multiplex methods and kits for detecting different analytes in a sample in parallel by sequential signal-encoding of said analytes, wherein the method allows a differentiation of targets which distance is below the diffraction limit of optical microscopes, that is, targets with spatial optical overlap. The disclosed methods also include in vitro methods for screening, identifying and/or testing a substance and/or drug and in vitro methods for diagnosis of a disease, and an optical multiplexing system.Type: ApplicationFiled: June 29, 2023Publication date: May 16, 2024Applicant: Resolve Biosciences GmbHInventors: Cheng Frank ZHONG, Christian Korfhage, Frank Reinecke, Stephan Tirier
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Publication number: 20230227907Abstract: The technology provided herein relates to multiplex methods and kits for detecting different analytes in a sample in parallel by sequential signal-encoding of said analytes, as well as in vitro methods for screening, identifying and/or testing a substance and/or drug and in vitro methods for diagnosis of a disease, and an optical multiplexing system.Type: ApplicationFiled: June 18, 2021Publication date: July 20, 2023Applicant: Resolve BioSciences GmbHInventors: Andreas Geipel, Frank Reinecke, Christian Korfhage
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Publication number: 20220235402Abstract: The present invention is directed to a method of sequential signal-encoding of analytes in a sample, a use of a set of decoding oligonucleotides to sequentially signal-encode analytes in a sample, and to a kit for sequentially signal-encoding of analytes in a sample.Type: ApplicationFiled: June 18, 2020Publication date: July 28, 2022Applicant: RESOLVE BIOSCIENCES GMBHInventors: Andreas GEIPEL, Frank REINECKE, Christian KORFHAGE, Nadine JAGEMANN, Vanessa MOELLERING
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Publication number: 20220205028Abstract: The technology provided herein relates to multiplex methods and kits for detecting different analytes and different subgroups/variations of an analyte in a sample, for example in parallel by sequential signal-encoding of said analytes, as well as in vitro methods for screening, identifying and/or testing a substance and/or drug and in vitro methods for diagnosis of a disease, and an optical multiplexing system.Type: ApplicationFiled: December 17, 2021Publication date: June 30, 2022Applicant: Resolve Biosciences GMBHInventors: Andreas GEIPEL, Christian KORFHAGE, Frank REINECKE
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Patent number: 11220705Abstract: The present invention is directed to a method for immobilizing nucleic molecule on solid support and to a use of a nucleic acid non-immobilized primer in combination with a nucleic acid primer linked to a solid support in said a method.Type: GrantFiled: April 25, 2016Date of Patent: January 11, 2022Assignee: QIAGEN GmbHInventors: Christian Korfhage, Evelyn Fricke
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Patent number: 11085073Abstract: The present invention is directed to a method for immobilizing a nucleic acid molecule on a solid support and to a use of a combination of a first nucleic acid immobilized primer linked to a solid support and a second immobilized primer linked to said solid support in said method.Type: GrantFiled: April 25, 2016Date of Patent: August 10, 2021Assignee: QIAGEN GmbHInventors: Christian Korfhage, Evelyn Fricke
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Patent number: 10087482Abstract: The present invention relates to a reaction mixture for the amplification of nucleic acids, the non-methylated cytosine bases of which have been converted to uracil bases by means of a bisulfition reaction. The invention also discloses methods for amplifying bisulfited nucleic acid and for determining the nucleic acid methylation state, and also kits based on the reaction mixture according to the invention.Type: GrantFiled: February 6, 2009Date of Patent: October 2, 2018Assignee: QIAGEN GMBHInventors: Christian Korfhage, Dirk Loeffert, Ralf Peist, Nicolas Rudinger
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Publication number: 20180112251Abstract: The present invention is directed to a method for immobilizing nucleic molecule on solid support and to a use of a nucleic acid non-immobilized primer in combination with a nucleic acid primer linked to a solid support in said a method.Type: ApplicationFiled: April 25, 2016Publication date: April 26, 2018Inventors: Christian KORFHAGE, Evelyn FRICKE
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Publication number: 20180105871Abstract: The present invention is directed to a method for immobilizing a nucleic acid molecule on a solid support and to a use of a combination of a first nucleic acid immobilized primer linked to a solid support and a second immobilized primer linked to said solid support in said method.Type: ApplicationFiled: April 25, 2016Publication date: April 19, 2018Inventors: Christian Korfhage, Evelyn Fricke
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Publication number: 20180087099Abstract: The present invention is directed to a method for hybridizing a nucleic acid molecule to another nucleic acid molecule and to a use of a nucleic acid molecule to reduce and/or avoid the formation of hairpin structures of a nucleic acid target molecule.Type: ApplicationFiled: April 25, 2016Publication date: March 29, 2018Inventors: Christian KORFHAGE, Evelyn FRICKE
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Patent number: 9777316Abstract: What is described is a method for quantitative and qualitative analysis of complex template nucleic acids to be analyzed. The method comprises the co-amplification of a control nucleic acid with the complex template nucleic acid by means of isothermal strand displacement reaction. The method of the invention further comprises the determination of the amount of amplified control nucleic acid as measure for the determination of the quantity and/or quality of the complex template nucleic acid used. The present invention also relates to a kit for carrying out a method of the invention. Furthermore, the use of the method of the invention or the kit of the invention for standardization of whole-genome, whole-transcriptome and whole-bisulfitome analyses is described.Type: GrantFiled: June 3, 2010Date of Patent: October 3, 2017Assignee: QIAGEN STRASSE 1Inventors: Christian Korfhage, Evelyn Fisch
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Patent number: 9683255Abstract: The present invention concerns a method for activating a nucleic acid for a polymerase reaction with the steps: (a) Heating a nucleic acid to a temperature of 55° C. to 80° C., (b) cooling the nucleic acid to a temperature at which a polymerase shows no substantial decrease in activity, and (c) starting the polymerase reaction by the addition of a heat-labile polymerase to the nucleic acid.Type: GrantFiled: September 11, 2006Date of Patent: June 20, 2017Assignee: QIAGEN GMBHInventors: Christian Korfhage, Dirk Löffert
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Patent number: 9611506Abstract: This invention relates to a process for synthesis of a cDNA in a sample, in an enzymatic reaction, whereby the process comprises the steps: simultaneous preparation of a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, a buffer, at least one ribonucleotide, at least one deoxyribonucleotide, an anchor oligonucleotide; addition of a sample that comprises a ribonucleic acid; and incubation of the agents of the previous steps in one or more temperature steps, which are selected such that the first enzyme and the second enzyme show activity. The invention further relates to a reaction mixture that comprises a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, optionally a buffer, optionally at least one ribonucleotide, optionally at least one deoxyribonucleotide, and optionally an anchor oligonucleotide. Moreover, the invention relates to a kit that comprises a corresponding reaction mixture.Type: GrantFiled: November 12, 2015Date of Patent: April 4, 2017Assignee: QIAGEN, GMBHInventors: Holger Engel, Subrahmanyam Yerramilli, Martin Kreutz, Dirk Loeffert, Christian Korfhage
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Publication number: 20160130634Abstract: This invention relates to a process for synthesis of a cDNA in a sample, in an enzymatic reaction, whereby the process comprises the steps: simultaneous preparation of a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, a buffer, at least one ribonucleotide, at least one deoxyribonucleotide, an anchor oligonucleotide; addition of a sample that comprises a ribonucleic acid; and incubation of the agents of the previous steps in one or more temperature steps, which are selected such that the first enzyme and the second enzyme show activity. The invention further relates to a reaction mixture that comprises a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, optionally a buffer, optionally at least one ribonucleotide, optionally at least one deoxyribonucleotide, and optionally an anchor oligonucleotide. Moreover, the invention relates to a kit that comprises a corresponding reaction mixture.Type: ApplicationFiled: November 12, 2015Publication date: May 12, 2016Inventors: Holger ENGEL, Subrahmanyam Yerramilli, Martin Kreutz, Dirk Loeffert, Christian Korfhage
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Patent number: 9217173Abstract: This invention relates to a process for synthesis of a cDNA in a sample, in an enzymatic reaction, wherein the process comprises the steps: simultaneous preparation of a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, a buffer, at least one ribonucleotide, at least one deoxyribonucleotide, an anchor oligonucleotide; addition of a sample that comprises a ribonucleic acid; and incubation of the agents of the previous steps in one or more temperature steps, which are selected such that the first enzyme and the second enzyme show activity. The invention further relates to a reaction mixture that comprises a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, optionally a buffer, optionally at least one ribonucleotide, optionally at least one deoxyribonucleotide, and optionally an anchor oligonucleotide. Moreover, the invention relates to a kit that comprises a corresponding reaction mixture.Type: GrantFiled: August 13, 2007Date of Patent: December 22, 2015Assignee: QIAGEN GMBHInventors: Holger Engel, Subrahmanyam Yerramilli, Martin Kreutz, Dirk Loeffert, Christian Korfhage
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Patent number: 9133508Abstract: A method is disclosed for improved isothermal amplification of nucleic acids comprising the step of release of an essential component from a matrix under predetermined conditions. Furthermore, the invention relates to a kit comprising mesophilic enzyme and a matrix with embedded essential components for isothermal amplification. A composition comprising a matrix and a mesophilic enzyme and a method for embedding a mesophilic enzyme are disclosed as well.Type: GrantFiled: August 9, 2011Date of Patent: September 15, 2015Assignee: Qiagen GmbHInventor: Christian Korfhage
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Patent number: 9074248Abstract: It has been unexpectedly and surprisingly found that Helicase Dependent Amplification (HDA) primers having termini enriched for A or C at the 5?-ends, result in a much more efficient HDA reaction than those primers having G or T rich 5?-ends. Since A is a low melting base and C is a high-melting base, the melting characteristic of primer termini is not correlated with melting characteristics of amplicon termini. Optimized HDA primers, methods of making and using optimized primers, as well as methods of as well as kits for optimized HDA are disclosed.Type: GrantFiled: December 18, 2012Date of Patent: July 7, 2015Assignee: QIAGEN GMBHInventors: Christian Korfhage, Sven van Ooyen
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Patent number: 9005933Abstract: The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.Type: GrantFiled: August 16, 2011Date of Patent: April 14, 2015Assignee: Qiagen GmbHInventors: Christian Korfhage, Gerd Grosshauser, Thomas Rothmann, Ralf Himmelreich
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Patent number: 8932831Abstract: The present invention concerns a method for inserting one or more tag sequences into a nucleic acid characterized by the following steps: (a) preparation of a template nucleic acid; (b) hybridization of at least one anchor sequence of at least one anchor oligonucleotide with one sequence section of the template nucleic acid; and (c) synthesization of a new strand of nucleic acid, which is partially complementary to the template nucleic acid and which contains a sequence complementary to the non-hybridized portion of the anchor oligonucleotide, e.g. to at least one tag sequence, on its 3? end.Type: GrantFiled: May 4, 2007Date of Patent: January 13, 2015Assignee: Qiagen GmbHInventors: Christian Korfhage, Holger Engel, Dirk Löffert, Ralf Peist
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Patent number: 8785120Abstract: The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo-oder heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carbonxylic acid amides, f) inorganic ammonium saType: GrantFiled: August 24, 2010Date of Patent: July 22, 2014Assignee: Qiagen GmbHInventors: Christian Korfhage, Friederike Wilmer, Dirk Loffert, Ralf Himmelreich, Claudia Fritz, Kathleen Rieske