Abstract: A light sheet microscope includes an illumination optical system configured to generate a light sheet in an intermediate image space. A transport optical system, which is telecentric on both sides, is configured to image the light sheet generated in the intermediate image space into a sample and to image a region of the sample illuminated by the light sheet as an intermediate image into the intermediate image space. A detection optical system is configured to image the intermediate image generated in the intermediate image space onto a detector. The optical axes of the illumination optical system, the transport optical system and the detection optical system intersect one another in the intermediate image space. The microscope further includes a scanning element, which is arranged in the transport optical system and through which the light sheet is moveable in the sample transversely to the optical axis of the transport optical system.

Abstract: A microscope includes at least one correction unit arranged in a beam path for correcting a variable spherical aberration. The correction unit has at least one optical correction element that is arranged in a convergent or divergent area of the beam path such that the optical correction element is movable along an optical axis. The at least one optical correction element has at least one correction surface. A part of the at least one correction surface through which the convergent or divergent area of the beam path passes forms a correction-effective surface section whose radial extension crosswise to the optical axis is adjustable by moving the correction element along the optical axis.

Abstract: An immersion objective lens for a microscope includes a first lens group having positive refractive power, a second lens group having positive refractive power, a third lens group having negative refractive power and a fourth lens group having positive refractive power disposed in this sequence from the object side. The second lens group is moveable along an optical axis so as to achieve a corrective effect with respect to a spherical aberration, such that a sum of a distance between the second lens group and the first lens group and of a distance between the second lens group and the third lens group is constant. The corrective effect of the second lens group is predetermined such that the spherical aberration is minimized for a light incidence that corresponds to a mean numerical aperture that lies between zero and a nominal aperture of the immersion objective lens.

Abstract: A fluorescence microscope has an incident fluorescence illumination unit generating an incident fluorescence illumination beam path a multi-band fluorescence filter system encompassing a multi-band beam splitter for deflecting the incident fluorescence illumination beam path into an objective of the microscope and onto a specimen. The filter system has a multi-band blocking filter that at least partly transmits a fluorescence emission beam path proceeding from the specimen; and having a digital camera for generating a fluorescence image of the specimen. The microscope has a bright-field transmitted illumination unit for generating a bright-field transmitted illumination beam path for transmitted illumination of the specimen. The filter system is arranged fixedly so that upon acquisition of a bright-field image of the specimen, the system remains in a bright-field transmitted light beam path proceeding from the specimen.

Abstract: An objective and an illumination unit for selectable generation of an orthoscopic beam path proceeding through the objective for pointlike scanning illumination, and of a conoscopic beam path proceeding through the objective for evanescent illumination of an object are disclosed. The illumination unit has a light source for generating illuminating rays along an illuminating beam path; a displacement unit for deflecting the illuminating beam path; a scanning eyepiece, placed after the displacement unit for focusing the illuminating rays into an image plane of the scanning eyepiece; and a mirror surface arranged in the image plane of the scanning eyepiece, having a transparent region for generating the orthoscopic beam path and having an at least partly reflective region facing toward the scanning eyepiece for generating the conoscopic beam path from the illuminating beam path, the image plane is located in a plane conjugated with the exit pupil.

Abstract: An illumination device for an optical device, a microscope or a macroscope includes a first illumination source configured to emit light which is directed via an illumination beam path onto an object to be illuminated that is arranged in an object plane. At least one second illumination source is positionable in the illumination beam path, and is transparent or semitransparent as well as self-luminous. The at least one second illumination source is configured to allow light emitted from the first illumination source to pass through at least in part. The object plane having the object to be illuminated is configured to be illuminated both by the first and by the at least one second illumination source.

Abstract: An optical arrangement in a microscope includes an illumination device configured to generate an illuminating light beam extending on an illumination side. A splitting device is configured to split the illuminating light beam into at least two partial beams. A mirror arrangement is configured to reflect the partial beams into an illumination region for plane illumination of a specimen. Detection optics are arranged on a side of the illumination region facing away from the illumination side.

Abstract: A microscope includes at least one correction unit arranged in a beam path for correcting a variable spherical aberration. The correction unit has at least one optical correction element that is arranged in a convergent or divergent area of the beam path such that the optical correction element is movable along an optical axis. The at least one optical correction element has at least one correction surface. A part of the at least one correction surface through which the convergent or divergent area of the beam path passes forms a correction-effective surface section whose radial extension crosswise to the optical axis is adjustable by moving the correction element along the optical axis.

Abstract: A method for microscopically imaging a volume sample includes focusing a microscope objective having a correcting element successively in at least two reference planes which are located within the volume sample at different volume sample depths along the optical axis of the microscope objective; determining, for each reference plane, a reference setting of the correcting element in which an imaging error which is dependent upon the volume sample depth is corrected by the correcting element; determining, on the basis of the reference settings determined for at least one target plane in the volume sample, a target setting for the correcting element in which the imaging error occurring at the volume sample depth of the target plane is corrected by the correcting element; and focusing the microscope objective on the target plane and bringing the correcting element into the target setting in order to image the volume sample.

Abstract: The present invention relates to a microscope (30) comprising an objective (1) and an illumination unit (31) for selectable generation of an orthoscopic beam path (14) proceeding through the objective (1) for pointlike scanning illumination, and of a conoscopic beam path (13) proceeding through the objective (1) for evanescent illumination of an object, the illumination unit comprising: a light source (10) for generating illuminating rays along an illuminating beam path (32); a displacement unit (7) for deflecting the illuminating beam path (32); a scanning eyepiece (8), placed after the displacement unit (7), for focusing the illuminating rays into an image plane (11) of the scanning eyepiece (8); and a mirror surface (6) arranged in the image plane (11) of the scanning eyepiece (8), having a transparent region (27) for generating the orthoscopic beam path (14) and having an at least partly reflective region (28) facing toward the scanning eyepiece (8) for generating the conoscopic beam path (13) from the il

Abstract: A process interface of a process gas analyzer operating by a transmitted light method includes a purging tube, which extends between an optoelectronic element and an interior of a plant part carrying a process gas, wherein the purging tube is closed off, at its end opposite from the optoelectronic element by a window, in the vicinity of which a purging gas feed enters the purging tube, where an annular part is arranged in the interior of the purging tube opposite the entrance of the purging gas feed and is coaxial in relation to the purging tube, and the part has a convex outer side, the vertex line of which divides the entrance of the purging gas feed into a smaller region, open toward the window, and a larger region, open toward the interior of the plant part.

Abstract: An optical arrangement in a microscope includes an illumination device configured to generate an illuminating light beam extending on an illumination side. A splitting device is configured to split the illuminating light beam into at least two partial beams. A mirror arrangement is configured to reflect the partial beams into an illumination region for plane illumination of a specimen. Detection optics are arranged on a side of the illumination region facing away from the illumination side.

Abstract: A process interface of a process gas analyzer operating by a transmitted light method includes a purging tube, which extends between an optoelectronic element and an interior of a plant part carrying a process gas, wherein the purging tube is closed off, at its end opposite from the optoelectronic element by a window, in the vicinity of which a purging gas feed enters the purging tube, where an annular part is arranged in the interior of the purging tube opposite the entrance of the purging gas feed and is coaxial in relation to the purging tube, and the part has a convex outer side, the vertex line of which divides the entrance of the purging gas feed into a smaller region, open toward the window, and a larger region, open toward the interior of the plant part.