Abstract: The invention relates generally to methods for purifying a Fc-fusion protein produced in a eukaryotic expression system. More specifically, the invention provides a robust and scalable downstream purification process suitable for use in manufacturing TNFR:Fc for human administration which comprises an optimized Protein A affinity chromatography step and two ion exchange chromatography steps both of which are operated in the bind-and-elute mode.

Abstract: The invention relates generally to methods for purifying a Fc-fusion protein produced in a eukaryotic expression system. More specifically, the invention provides a robust and scalable downstream purification process suitable for use in manufacturing TNFR:Fc for human administration which comprises an optimized Protein A affinity chromatography step and two ion exchange chromatography steps both of which are operated in the bind-and-elute mode.

Abstract: This is directed to a system and method for providing social networking services using a portable electronic device. In some embodiments, a user may identify one or more articles of interest and transmit identifying information for the articles to mobile or other devices of the user's friends. The user's friends can review the identified articles, and provide comments for the user. For example, the user's friends can give a thumbs up/down, a star rating, a comment (e.g., text, audio or video), or any other type of comment. In some embodiments, the user can request the assistance of a salesperson using the social networking application device, for example to request recommended articles based on a particular event or need. In some embodiments, the social networking application can provide information to or from a kiosk.