Abstract: The use of acoustic focusing together with a non-destructive extraction process for obtaining lipids from oleaginous algae is described. Acoustic focusing can be used to concentrate oleaginous algae before they are subjected to non-destructive extraction, or acoustic focusing can be used as part of the non-destructive extraction process itself by moving the algae from the algae culture to the extracting solvent. The use of acoustic focusing for removing extraneous particulate matter from algae cultures is also described, as well as the use of non-destructive extraction for controlling levels of algae predators.