Abstract: A single cell isolation apparatus is provided having a base plate with at least one micromachined well therein, a micromachined membrane, and a housing for holding the other components of the apparatus. The apparatus also may include a gel deposited above the base plate, a second membrane intermediate the base plate and the gel, and a filter above the gel, with the micromachined membrane being above the filter. Each micromachined well associated with the apparatus has a tapered sidewall, with the upper diameter corresponding to the average size of the type of cell attempting to be isolated through the use of the apparatus, and a lower diameter which is less than the upper diameter. The gel component of the apparatus is a nitrocellulose gel. The filter is permeable to cellular products, and preferably is fabricated from paper. The second membrane provides fluid for the gel necessary for maintaining the gel's physical properties.