Abstract: A range of Cel7 putative cellobiohydrolase genes were identified using genome mining and homologous sequence alignment to Cel7A from Trichoderma reesei. A representative subset of these genes from across a broad diversity of evolutionarily disparate sources were cloned and expressed in T. reesei using a constitutive promotor and a common secretion signal. The purified recombinant enzymes were tested for efficacy on various substrates. The top performers were subjected to structural studies and subsites likely to confer enhanced performance were predicted using homology modeling and comparisons of natural sequence diversity. Once identified, the subsites were genetically introduced individually and combinatorically into the best in class Cel7A backbone we have found to date and then expressed in T. reesei and tested. A triple mutant was determined to have the highest cellulase activity we have measured in a cellobiohydrolase to date.

Abstract: A system and method for constructing a graphical user interface for an application being accessed by a user are provided. The method includes measuring the user's current work rate, deriving a threshold from the user's current work rate, determining the user's current activity within the application, assigning a value to the user's current activity, accessing a model for the application, the model defining links between activities within the application, assigning values to activities linked within the accessed model to the user's current activity, and displaying a control element in a graphical user interface for each activity with a value above the derived threshold.

Abstract: A system and method for constructing a graphical user interface for an application being accessed by a user are provided. The method includes measuring the user's current work rate, deriving a threshold from the user's current work rate, determining the user's current activity within the application, assigning a value to the user's current activity, accessing a model for the application, the model defining links between activities within the application, assigning values to activities linked within the accessed model to the user's current activity, and displaying a control element in a graphical user interface for each activity with a value above the derived threshold.

Abstract: A wheelchair suitable for invalids or geriatric patients has a chair or seat supported on independant forward and rearward castor frames pivotally connected together at a transverse axis to form a castor frame assembly. The chair is pivotally mounted on the forward and rearward castor frames so that in negotiating a step or other obstacle, the lifting movement of the occupant is divided into stages so that a pleasant or comfortable ride is obtained. The wheelchair may be folded into a collapsed condition with the chair itself located between the castor frames.