Patents by Inventor Christopher M. Puleo

Christopher M. Puleo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Microfluidic system for high-throughput, droplet-based single molecule analysis with low reagent consumption
    Patent number: 9284601
    Abstract: A microfluidic device for a confocal fluorescence detection system has an input channel defined by a body of the microfluidic device, a sample concentration section defined by the body of the microfluidic device and in fluid connection with the input channel, a mixing section defined by the body of the microfluidic device and in fluid connection with the concentration section, and a detection region that is at least partially transparent to illumination light of the confocal fluorescence detection system and at least partially transparent to fluorescent light when emitted from a sample under observation as the sample flows through the detection region.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: March 15, 2016
    Assignee: The Johns Hopkins University
    Inventors: Tza-Huei Wang, Kelvin J. Liu, Christopher M. Puleo, Tushar D. Rane
  • MICROFLUIDIC SYSTEM FOR HIGH-THROUGHPUT, DROPLET-BASED SINGLE MOLECULE ANALYSIS WITH LOW REAGENT CONSUMPTION
    Publication number: 20140106462
    Abstract: A microfluidic device for a confocal fluorescence detection system has an input channel defined by a body of the microfluidic device, a sample concentration section defined by the body of the microfluidic device and in fluid connection with the input channel, a mixing section defined by the body of the microfluidic device and in fluid connection with the concentration section, and a detection region that is at least partially transparent to illumination light of the confocal fluorescence detection system and at least partially transparent to fluorescent light when emitted from a sample under observation as the sample flows through the detection region.
    Type: Application
    Filed: December 20, 2013
    Publication date: April 17, 2014
    Applicant: The Johns Hopkins University
    Inventors: Jeff T. Wang, Kelvin J. Liu, Christopher M. Puleo, Tushar D. Rane
  • Microfluidic solution for high-throughput, droplet-based single molecule analysis with low reagent consumption
    Patent number: 8637301
    Abstract: A microfluidic device for a confocal fluorescence detection system has an input channel defined by a body of the microfluidic device, a sample concentration section defined by the body of the microfluidic device and in fluid connection with the input channel, a mixing section defined by the body of the microfluidic device and in fluid connection with the concentration section, and a detection region that is at least partially transparent to illumination light of the confocal fluorescence detection system and at least partially transparent to fluorescent light when emitted from a sample under observation as the sample flows through the detection region.
    Type: Grant
    Filed: March 2, 2010
    Date of Patent: January 28, 2014
    Assignee: The Johns Hopkins University
    Inventors: Jeff Tza-Huei Wang, Kelvin J. Liu, Christopher M. Puleo, Tushar D. Rane
  • SINGLE MOLECULE SPECTROSCOPY FOR ANALYSIS OF CELL-FREE NUCLEIC ACID BIOMARKERS
    Publication number: 20120135874
    Abstract: The present invention relates, e.g., to a method for detecting a nucleic acid molecule of interest in a sample comprising cell-free nucleic acids, comprising fluorescently labeling the nucleic acid molecule of interest, by specifically binding a fluorescently labeled nanosensor or probe to the nucleic acid of interest, or by enzymatically incorporating a fluorescent probe or dye into the nucleic acid of interest, illuminating the fluorescently labeled nucleic acid molecule, causing it to emit fluorescent light, and measuring the level of fluorescence by single molecule spectroscopy, wherein the detection of a fluorescent signal is indicative of the presence of the nucleic acid of interest in the sample.
    Type: Application
    Filed: May 6, 2010
    Publication date: May 31, 2012
    Applicant: The Johns Hopkins University
    Inventors: Jeff Tza-Huei Wang, Kelvin J. Liu, Christopher M. Puleo
  • MICROFLUIDIC SOLUTION FOR HIGH-THROUGHPUT, DROPLET-BASED SINGLE MOLECULE ANALYSIS WITH LOW REAGENT CONSUMPTION
    Publication number: 20110287976
    Abstract: A microfluidic device for a confocal fluorescence detection system has an input channel defined by a body of the microfluidic device, a sample concentration section defined by the body of the microfluidic device and in fluid connection with the input channel, a mixing section defined by the body of the microfluidic device and in fluid connection with the concentration section, and a detection region that is at least partially transparent to illumination light of the confocal fluorescence detection system and at least partially transparent to fluorescent light when emitted from a sample under observation as the sample flows through the detection region.
    Type: Application
    Filed: March 2, 2010
    Publication date: November 24, 2011
    Inventors: Jeff Tza-Huei Wang, Kelvin J. Liu, Christopher M. Puleo, Tushar D. Rane