Abstract: An organic microgel system as support material for 3D printing of soft materials such as silicone and methods for manufacturing and using the organic microgel system are disclosed. In some embodiments, the organic microgel system comprises a plurality of microgel particles formed by blending a di-block copolymer and a tri-block copolymer in an organic solvent. The organic microgel system may allow high precision 3D printing of silicone objects with complex shapes.

Abstract: Described herein are compositions, methods, kits, and systems relating to smooth, spherical microgels which can be charge-neutral. The microgels can be made using an emulsification process. In certain aspects, charge-neutral microgels as described herein are suitable for 3D cell culture, use in perfusion bioreactors, and/or 3D printing of cells for 3D cell culture.

Abstract: Described herein are systems and methods relating to three-dimensional (3D) cell manufacturing utilizing a 3D growth media. According to aspects of the present disclosure, pluralities of cells and ECM components can be printed into a bioreactor as a sheet. Sheets can be retrieved, digested, split, and re-printed, thereby expanding the number of cells in an efficient manner that conserves space in a tissue culture incubator, conserves bioreactor consumables, and consumes nutrients required for cell maintenance and growth.

Abstract: An organic microgel system as support material for 3D printing of soft materials such as silicone and methods for manufacturing and using the organic microgel system are disclosed. In some embodiments, the organic microgel system comprises a plurality of microgel particles formed by blending a di-block copolymer and a tri-block copolymer in an organic solvent. The organic microgel system may allow high precision 3D printing of silicone objects with complex shapes.

Abstract: An organic microgel system as support material for 3D printing of soft materials such as silicone and methods for manufacturing and using the organic microgel system are disclosed. In some embodiments, the organic microgel system comprises a plurality of microgel particles formed by blending a di-block copolymer and a tri-block copolymer in an organic solvent. The organic microgel system may allow high precision 3D printing of silicone objects with complex shapes.

Abstract: Microgel particles for use in a three-dimensional cell growth medium are described. The microgel particles may be swellable and may have properties conducive to improved function and health of cells distributed within the three-dimensional cell growth medium. Related compositions, systems, and methods are also described. Also provided is a plurality of microgel particles and a liquid cell culture medium, wherein the microgel particles are swelled with the liquid cell culture medium to form a granular gel. Also provided is a method of preparing a three-dimensional cell growth medium is disclosed. The method may comprise: mixing a plurality of microgel particles, such as those described above, in a liquid cell culture medium. Also provided is a method of placing cells in a three-dimensional cell growth medium is disclosed. Also provided is a method of synthesizing a protein is disclosed.

Abstract: An organic microgel system as support material for 3D printing of soft materials such as silicone and methods for manufacturing and using the organic microgel system are disclosed. In some embodiments, the organic microgel system comprises a plurality of microgel particles formed by blending a di-block copolymer and a tri-block copolymer in an organic solvent. The organic microgel system may allow high precision 3D printing of silicone objects with complex shapes.