Abstract: A device may receive input data associated with a legal regulation, and may process the input data to generate a record that includes: the input data in a knowledge representation format and a semantic representation format, data identifying a feature, data identifying an industry classification, or data identifying an entity of interest. The device may process the record, with machine learning models, to determine output data that includes: data indicating that the legal regulation is inconsistent, data indicating that the legal regulation is outdated, data indicating a sentiment for the legal regulation, data indicating a prescriptive nature of the legal regulation, data indicating a complexity of the legal regulation, data indicating a misrepresentation in the legal regulation, data indicating a compliance burden associated with the legal regulation, or data indicating an industry performance impact of the legal regulation. The device may perform actions based on the output data.

Abstract: The disclosure provides ?-ketoisocaproic acid decarboxylase and ?-keto-3-methylvaleric acid decarboxylase and recombinant microorganisms that host these enzymes. Methods involve the use of recombinant microorganisms to increase the production of isoamyl alcohols, their corresponding acids and their derivatives from carbon sources.

Abstract: A device may receive input data associated with a legal regulation, and may process the input data to generate a record that includes: the input data in a knowledge representation format and a semantic representation format, data identifying a feature, data identifying an industry classification, or data identifying an entity of interest. The device may process the record, with machine learning models, to determine output data that includes: data indicating that the legal regulation is inconsistent, data indicating that the legal regulation is outdated, data indicating a sentiment for the legal regulation, data indicating a prescriptive nature of the legal regulation, data indicating a complexity of the legal regulation, data indicating a misrepresentation in the legal regulation, data indicating a compliance burden associated with the legal regulation, or data indicating an industry performance impact of the legal regulation. The device may perform actions based on the output data.

Abstract: In an intake manifold that has charge-motion-control valves (CMCVs), a gap exists between the flapper valves and the wall of the intake runners. Although the gap is maintained as small as practical, it cannot be eliminated because manufacturing tolerances and temperature variations to which the intake manifold is subjected must be accommodated to prevent binding of the flapper valves. Some flow makes an end run through the gap leading to undesirable fluid mechanics. Disclosed herein is a seal that is positioned to rest gently upon the flapper valve near the gap such that the gap is substantially sealed off while applying a modest force on the CMCV so that the actuation torque is minimally impacted. The seal has a press-in-place portion inserted into a pocket formed in the manifold to hold it in place and a lip portion that extends out from the press-in-place portion to obstruct the gap.

Abstract: In an intake manifold that has charge-motion-control valves (CMCVs), a gap exists between the flapper valves and the wall of the intake runners. Although the gap is maintained as small as practical, it cannot be eliminated because manufacturing tolerances and temperature variations to which the intake manifold is subjected must be accommodated to prevent binding of the flapper valves. Some flow makes an end run through the gap leading to undesirable fluid mechanics. Disclosed herein is a seal that is positioned to rest gently upon the flapper valve near the gap such that the gap is substantially sealed off while applying a modest force on the CMCV so that the actuation torque is minimally impacted. The seal has a press-in-place portion inserted into a pocket formed in the manifold to hold it in place and a lip portion that extends out from the press-in-place portion to obstruct the gap.

Abstract: The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise at least one nucleic acid molecule encoding a polypeptide with keto-isovalerate decarboxylase (KIVD) activity, wherein said polypeptide is at least about 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to a polypeptide selected from SEQ ID NOs: 1-214. Also provided are modified decarboxylases exhibiting an improved ability to utilize ?-ketoisovalerate as a substrate in various beneficial enzymatic conversions.

Abstract: The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or a modified NADH-dependent variant thereof, wherein said KARI is at least about 80% identical to SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 28, SEQ ID NO: 40, or SEQ ID NO: 58. The present invention also relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or a modified NADH-dependent variant thereof, wherein said KARI is at least about 99% identical to SEQ ID NO: 64. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.

Abstract: The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or modified NADH-dependent variant thereof, wherein said KARI is at least about 60% identical to SEQ ID NO: 2. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.

Abstract: A composite intake manifold coupled to a V-engine is provided. The composite intake manifold includes a supporting member overmolded in an unsupported region of the composite intake manifold.

Abstract: A composite intake manifold coupled to a V-engine is provided. The composite intake manifold includes a supporting member overmolded in an unsupported region of the composite intake manifold.

Abstract: A composite intake manifold coupled to a V-engine is provided. The composite intake manifold includes a supporting member overmolded in an unsupported region of the composite intake manifold.

Abstract: A composite intake manifold coupled to a V-engine is provided. The composite intake manifold includes a supporting member overmolded in an unsupported region of the composite intake manifold.

Abstract: An optical illumination and monitoring subsystem controls the operation of a flow cytometer having a carrier fluid that flows along a channel coupled to a droplet generator, which controls a point at which droplets break off from the carrier fluid, and a droplet sorter that is operative to cause selected droplets to be sorted along one or more droplet travel paths. The subsystem is operative to illuminate a respective droplet monitoring location along each of one or more droplet travel paths with a respective beam of light, such as that sourced from a common laser. In response to backscatter reflection from a droplet passing through the respective beam of light at a respective droplet monitoring location, the amplitude of the beam of light is increased. Then the droplet is monitored for the presence of a particle therein exhibiting detectable fluorescence as a result of the increase in the amplitude of the respective beam of light.

Abstract: A fitment removal prevention device for insertion within a container having a rigid mouth portion. The device has a body portion and an incompressible member movable with respect to the body portion and biased from a first position, in which the device is insertable into the container, to a second position, in which the incompressible member engages the container and exerts a force on the mouth portion of the container in a first direction having a component transverse to the direction of extraction of the device from the container. Upon attempted extraction of the fitment removal prevention device from the container, the incompressible member exerts an increased force on the mouth portion of the container in the first direction, thereby retaining the device in the container.