Abstract: A membranous immobilized cell, polypeptide, oligopeptide or protein and a preparation method thereof are provided. The method includes the following steps: 1) providing un-film-form chitosan, where the chitosan is un-pre-crosslinked or pre-crosslinked; 2) providing a mixture of the un-film-form chitosan and cell, polypeptide, oligopeptide or protein, and in the mixture, the un-film-form chitosan is in a dissolved state; 3) mixing the mixture with a crosslinking reagent to obtain a co-crosslinked product of the chitosan and the cell, polypeptide, oligopeptide or protein; and 4) drying the co-crosslinked product to obtain membranous immobilized cell, polypeptide, oligopeptide or protein. When un-pre-crosslinked chitosan is used in the step 1), the method further includes comprises the step 5) mixing the membranous immobilized cell, polypeptide, oligopeptide or protein with phosphate salt, so that chitosan molecules therein are crosslinked with each other.

Abstract: Provided herein are a device and a method for preparation of immobilized proteins, enzymes or cells on a carrier to achieve the industrial batch production of the immobilized proteins, enzymes or cells.

Abstract: Provided herein are a device and a method for preparation of immobilized proteins, enzymes or cells on a carrier to achieve the industrial batch production of the immobilized proteins, enzymes or cells.

Abstract: The invention is directed to a complex, including: a porous composite carrier including: a porous organic foam material containing open pores, each pore comprising a wall defining the pore; and a crosslinked product having aldehyde groups and immobilized on the surface of the walls of one or more pores of the porous organic foam materials, and a protein, polypeptide, or oligopeptide immobilized onto the porous composite carrier through a reaction between an amino group of the protein, polypeptide or oligopeptide and an aldehyde group of the composite carrier. The immobilized product has high specific surface area and high specific activity. The immobilization is simple and in low cost, and is suitable for industrial application.

Abstract: Provided herein are a device and a method for preparation of immobilized proteins, enzymes or cells on a carrier to achieve the industrial batch production of the immobilized proteins, enzymes or cells.

Abstract: Provided are a composite carrier for immobilization of a protein, polypeptide or oligopeptide, a preparation method and an application thereof. The composite carrier is a porous material which comprises: (1) a porous organic foam material containing open pores; and (2) a crosslinked product having aldehyde groups and immobilized on the surface of the walls of one or more pores of the porous organic foam material, wherein the aldehyde groups are able to react with the protein, polypeptide or oligopeptide, and the crosslinked product are formed by chitosan via a crosslinking reaction with a polyaldehyde compound.