Abstract: Provided are a genetically modified cell line producing a recombinant glycoprotein having a mannose-terminated N-glycan and a method for producing the recombinant glycoprotein or a method for generating the cell, clone or cell line. The cell line comprises an insertion of less than 600 bp in the coding region of a chromosomal sequence encoding MGAT1.

Abstract: A method for preparing a tar extract with aroma characteristics from a discarded cigarette butt includes: (1) adding the discarded cigarette butt to an extraction solvent, and carrying out subcritical fluid extraction to obtain an extraction solution; (2) carrying out vacuum filtration on the extraction solution, and carrying out vacuum distillation on the filtrate to obtain a concentrated extractum; and (3) carrying out molecular distillation on the concentrated extractum, and collecting a light fraction of the molecular distillation to obtain the tar extract with aroma characteristics. Through the subcritical fluid extraction and separation by the molecular distillation to prepare the tar extract, harmful substances in the tar are removed, and the aroma components are retained. The tar extract is applied to a cigarette to improve the aroma, smoke and taste characteristics of the cigarette.

Abstract: A method for preparing a tar extract with aroma characteristics from a discarded cigarette butt includes: (1) adding the discarded cigarette butt to an extraction solvent, and carrying out subcritical fluid extraction to obtain an extraction solution; (2) carrying out vacuum filtration on the extraction solution, and carrying out vacuum distillation on the filtrate to obtain a concentrated extractum; and (3) carrying out molecular distillation on the concentrated extractum, and collecting a light fraction of the molecular distillation to obtain the tar extract with aroma characteristics. Through the subcritical fluid extraction and separation by the molecular distillation to prepare the tar extract, harmful substances in the tar are removed, and the aroma components are retained. The tar extract is applied to a cigarette to improve the aroma, smoke and taste characteristics of the cigarette.

Abstract: The present disclosure provides a method for extracting tar aroma components from a cigarette butt and use of the tar aroma components in a cigarette. The method includes the following steps: (1) adding the cigarette butt to an extraction solvent, and carrying out ultrasound-assisted extraction to obtain an extraction solution; (2) centrifuging the extraction solution, and carrying out vacuum distillation on the supernatant to obtain a concentrated extractum; and (3) carrying out molecular distillation on the concentrated extractum, and collecting a light fraction of the molecular distillation to obtain the tar aroma components. In the present disclosure, the cigarette butt is used as a raw material, through the ultrasound-assisted extraction and separation by the molecular distillation to extract the tar, harmful substances in the tar are removed, and the aroma components are retained.

Abstract: A method for preparing a tar extract from a discarded cigarette butt and use of the tar extract in a cigarette includes the following steps: (1) adding the discarded cigarette butt to an extraction solvent, and carrying out microwave-assisted extraction to obtain an extraction solution; and (2) centrifuging the extraction solution, carrying out silica gel column chromatography on the supernatant, carrying out elution by using a petroleum ether-ethyl acetate mixed solution as a mobile phase, and collecting the resulting elution fraction to obtain the tar extract. In the method, the tar is extracted from the discarded cigarette butt as a raw material. Through the microwave-assisted extraction and separation by the silica gel column chromatography, harmful substances in the tar of the cigarette butt are removed, and the components with aroma characteristics are retained, and the tar extract is applied to a low-end cigarette.

Abstract: A magnetic material having dual properties and a manufacturing method thereof are disclosed. The magnetic material having dual properties consists of: 5% to 88% of a permanent magnetic material, 5% to 88% of a soft magnetic material, 6% to 16% of a binder, and 1% to 10% of an auxiliary agent. The magnetic material having dual properties manufactured by mixing two phases without microscopic intergranular exchange coupling interaction has unexpected effects: the range of a magnetically attracted object is expanded to include a magnet having dual properties; the range of a magnetically attractive object is expanded to include a magnet having dual properties; the minimum value of the magnetic attraction force is increased, the magnetic attraction force is more uniform, and it is smoother to move and rotate an object. The effect obtained by two layers of the soft magnet and the permanent magnet can be realized by a single layer structure of the magnet having dual properties.

Abstract: The present disclosure relates to a soft magnetic composite with a two-dimensional magnetic moment and a high working frequency band, and a preparation method therefor. According to an embodiment, the soft magnetic composite with a two-dimensional magnetic moment may comprise: an insulating matrix; and two-dimensional magnetic moment micropowder dispersed in the insulating matrix, wherein inside the two-dimensional magnetic moment micropowder, a magnetic moment is distributed in a specific two-dimensional plane.

Abstract: An amphoteric magnetic material and a manufacturing method thereof are disclosed. The amphoteric magnetic material consists of: 5% to 88% of a permanent magnetic material, 5% to 88% of a soft magnetic material, 6% to 16% of a binder, and 1% to 10% of an auxiliary agent. The amphoteric magnet manufactured by mixing two phases without microscopic intergranular exchange coupling interaction has unexpected effects: the range of a magnetically attracted object is expanded to include an amphoteric magnet; the range of a magnetically attractive object is expanded to include an amphoteric magnet; the minimum value of the magnetic attraction force is increased, the magnetic attraction force is more uniform, and it is smoother to move and rotate an object. The effect obtained by two layers of the soft magnet and the permanent magnet can be realized by a single layer structure of the amphoteric magnet.

Abstract: A method for preparing fermentation broth of fruits and vegetables includes steps of mixing fruits, vegetables, bacteria liquid of Lactobacillus acidophilus, bacteria liquid of Bifidobactreium longum, bacteria liquid of Lactobacillus delbrueckii subsp. bulgaricus, and bacteria liquid of Streptococcus thermophilus, and processing fermentation to obtain the fermentation broth of fruits and vegetables. A fermenting course of the method could be controlled, and a fermenting period is reduced to 15 days.

Abstract: A pharmaceutical composition for relieving fatigue and preparation method thereof are provided. The pharmaceutical composition is prepared by mixing ethanol extract of ginseng, ethanol extract of astragalus, ethanol extract of epimedium, ethanol extract of yam, ethanol extract of polygonatum, ethanol extract of dodder, aqueous extract of wolfberry and a fermentation conversion solution of fruits and vegetables.

Abstract: A method for preparing a pharmaceutical composition for enhancing immunity and a pharmaceutical composition prepared according to the method. The method includes steps of: mixing ginseng ethanol extract with fruit and vegetable fermentation conversion solution to obtain a mixture, namely, the pharmaceutical composition for enhancing immunity; wherein the ginseng ethanol extract is prepared by a following step of: reflux extracting ginseng by ethanol aqueous solution for obtaining the extract, namely, the ginseng ethanol extract; the fruit and vegetable fermentation conversion solution is prepared by following steps of: mixing fruit and vegetable, lactobacillus acidophilus bacteria solution, bifidobactreium longum bacteria solution, lactobacillus delbrueckii subsp. bulgaricus bacteria solution, streptococcus thermophilus bacteria solution with water, and then fermenting for obtaining a fermentation product, namely, the fruit and vegetable fermentation conversion solution.

Abstract: A pharmaceutical composition for strengthening spleen and stomach and a preparation method therefor. The method includes the step of mixing four substances listed below to obtain a mixture, wherein the mixture is the pharmaceutical composition for strengthening spleen and stomach, and the four substances are (A) ginseng ethanol extract, (B) volatile oil of largehead atractylodes rhizome and tangerine peels, (C) aqueous extract of Indian bread, yamaimo, lotus seeds, tangerine peels, liquorice root, hawthorn fruit, germinated barley, largehead atractylodes rhizome and rhizoma alismatis; (D) fermentation broth of fruits and vegetables.

Abstract: A method for preparing a pharmaceutical composition for enhancing immunity and a pharmaceutical composition prepared according to the method. The method includes steps of: mixing ginseng ethanol extract with fruit and vegetable fermentation conversion solution to obtain a mixture, namely, the pharmaceutical composition for enhancing immunity; wherein the ginseng ethanol extract is prepared by a following step of: reflux extracting ginseng by ethanol aqueous solution for obtaining the extract, namely, the ginseng ethanol extract; the fruit and vegetable fermentation conversion solution is prepared by following steps of: mixing fruit and vegetable, lactobacillus acidophilus bacteria solution, bifidobactreium longum bacteria solution, lactobacillus delbrueckiisubsp. bulgaricus bacteria solution, streptococcus thermophilus bacteria solution with water, and then fermenting for obtaining a fermentation product, namely, the fruit and vegetable fermentation conversion solution.

Abstract: A method for preparing fermentation broth of fruits and vegetables includes steps of mixing fruits, vegetables, bacteria liquid of Lactobacillus acidophilus, bacteria liquid of Bifidobactreium longum, bacteria liquid of Lactobacillus delbrueckii subsp. bulgaricus, and bacteria liquid of Streptococcus thermophilus, and processing fermentation to obtain the fermentation broth of fruits and vegetables. A fermenting course of the method could be controlled, and a fermenting period is reduced to 15 days.

Abstract: Pharmaceutical composition for relieving fatigue and preparation method thereof. A pharmaceutical composition for relieving fatigue and preparation method thereof are provided. The pharmaceutical composition is prepared by mixing ethanol extract of ginseng, ethanol extract of astragalus, ethanol extract of epimedium, ethanol extract of yam, ethanol extract of polygonatum, ethanol extract of dodder, aqueous extract of wolfberry and a fermentation conversion solution of fruits and vegetables.

Abstract: A pharmaceutical composition for strengthening spleen and stomach and a preparation method therefor. The method includes the step of mixing four substances listed below to obtain a mixture, wherein the mixture is the pharmaceutical composition for strengthening spleen and stomach, and the four substances are (A) ginseng ethanol extract, (B) volatile oil of largehead atractylodes rhizome and tangerine peels, (C) aqueous extract of Indian bread, yamaimo, lotus seeds, tangerine peels, liquorice root, hawthorn fruit, germinated barley, largehead atractylodes rhizome and rhizoma alismatis; (D) fermentation broth of fruits and vegetables.

Abstract: A pharmaceutical composition for treating melasma is provided. The pharmaceutical composition is prepared from ginseng, salvia, mint, orange peel, peony, poria, licorice, peach seed, yam, penthorum chinense and a fermentation conversion solution of fruits and vegetables. A preparation method of the pharmaceutical composition is also provided.