Abstract: Recombinant DNA constructs that provide for expression of desired target proteins as protein bodies in various host cells, tissues, and organisms are disclosed. Such recombinant DNA constructs contain a heterologous polynucleotide comprising an ER luminal folding chaperone interacting domain (CID), a sequence that codes for a trans-membrane domain (TMD); and a sequence that codes for a protein-protein interaction domain (PPID) that are operably linked to a sequence encoding the desired target protein. Proteins obtained from the constructs, host cells containing the constructs, and methods for obtaining desired target proteins encoded by the constructs are also disclosed.

Abstract: The invention describes methods for producing plant resistance to a ssDNA virus, particularly a geminivirus such as mastrevirus, curtovirus or begomovirus. The method comprises introducing a ssDNA-binding protein of the Inoviridae virus into the plant, and includes a phage coat protein, particularly, a coliphage gene 5 protein. The invention also describes a transgenic plant comprising a gene that expresses the ssDNA-binding protein and vectors for expressing the protein in plants.

Abstract: The present invention relates to short interfering RNAs (siRNAs) and methods of using the same to suppress or inhibit gene expression in plant cells. In particular, the present invention relates to siRNAs and methods that can be used to suppress or inhibit viral gene expression in plant cells. Still more particularly, the present invention relates to siRNAs and methods that can be used to suppress or inhibit geminiviral gene expression in plant cells. Of course, the siRNAs of the present invention could be used to generate plants that are resistant to geminiviral infection. In addition, methods of identifying specific compositions, or siRNAs, that can be used to inhibit gene expression in plant cells generally are provided.