Abstract: A liquid-phase preparative chromatographic apparatus comprising a column in the form of a cylindrical tube equipped at its lower section with a cap; a filling device to be connected by means of a compression nozzle and comprising a cylinder for actuating a non-porous solid body that compresses the stationary phase with which the column is filled by means of the compression nozzle; an injection head to be fastened onto the column after it is filled and comprising a lower wall and an injection unit having a diameter smaller than the injection head and equipped with several lateral solute supply ports, the head comprising on its upper surface a central solvent supply port and means enabling the solvent to flow around the injection unit; a pump connected to the injection head and comprising four pistons activated by cams or rods disposed at 90.degree.