Patents by Inventor Claus von der Osten
Claus von der Osten has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8486681Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: GrantFiled: January 27, 2009Date of Patent: July 16, 2013Assignee: Novozymes A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus Von Der Osten
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Publication number: 20120289450Abstract: The present invention relates to cellulase variants, i.e., endo-beta-1,4-glucanase variants, derived from a parental cellulase, i.e., endo-beta-1,4-glucanase, by substitution, insertion and/or deletion, which variant has a catalytic core domain, in which the variant at position 5 holds an alanine residue (A), a serine residue (S), or a threonine residue (T); at position 8 holds a phenylalanine residue (F), or a tyrosine residue (Y); at position 9 holds a phenylalanine residue (F), a tryptophan residue (W), or a tyrosine residue (Y); at position 10 holds an aspartic acid residue (D); and at position 121 holds an aspartic acid residue (D).Type: ApplicationFiled: May 15, 2012Publication date: November 15, 2012Applicant: NOVOZYMES A/SInventors: Kim Vilbour Andersen, Martin Schülein, Lars Christiansen, Bo Damgaard, Claus Von der Osten
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Patent number: 8252573Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: GrantFiled: February 3, 2011Date of Patent: August 28, 2012Assignee: Novozymes A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus Von Der Osten
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Publication number: 20120208251Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: ApplicationFiled: April 25, 2012Publication date: August 16, 2012Applicant: NOVOZYMES A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus Von Der Osten
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Publication number: 20120165241Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.Type: ApplicationFiled: March 7, 2012Publication date: June 28, 2012Applicants: UNILEVER PLC, NOVOZYMES A/SInventors: Laurens Nicolaas Sierkstra, Jan Klugkist, Peter Markvardsen, Claus von der Osten, Peter Bauditz
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Publication number: 20110250674Abstract: The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 ?; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g.Type: ApplicationFiled: June 17, 2011Publication date: October 13, 2011Applicant: Novozymes A/SInventors: Kim Vilbour Andersen, Martin Schülein, Torben Henriksen, Lars Christiansen, Bo Damgaard, Claus Von der Osten
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Publication number: 20110230386Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.Type: ApplicationFiled: May 25, 2011Publication date: September 22, 2011Applicants: Novozymes A/S, Unilever PLCInventors: Laurens Nicolaas Sierkstra, Jan Klugkist, Peter Markvardsen, Claus Von der Osten, Peter Bauditz
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Patent number: 8017372Abstract: The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 ?; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g.Type: GrantFiled: July 30, 2007Date of Patent: September 13, 2011Assignee: Novozymes A/SInventors: Kim Vilbour Andersen, Martin Schulein, Torben Henriksen, legal representative, Lars Christiansen, Bo Damgaard, Claus Von der Osten
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Patent number: 7993898Abstract: The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 ?; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g.Type: GrantFiled: February 27, 2009Date of Patent: August 9, 2011Assignee: Novozymes A/SInventors: Kim Vilbour Andersen, Martin Schulein, Torben Henriksen, legal representative, Lars Christensen, Bo Damgaard, Claus Von der Osten
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Publication number: 20110152155Abstract: The present invention relates to novel subtilase variants exhibiting alterations relative to the parent subtilase in one or more properties including: Wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash compositions, including automatic dishwash compositions.Type: ApplicationFiled: March 2, 2011Publication date: June 23, 2011Applicant: Novozymes A/SInventors: Tina Sejersgård Fanø, Claus Von Der Osten, Malene Kappen Krüger, Mads Nørregaard-Madsen
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Publication number: 20110136206Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: ApplicationFiled: February 3, 2011Publication date: June 9, 2011Applicant: Novozymes A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus Von der Osten
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Publication number: 20110045572Abstract: The present invention relates to a method of selecting a protein variant having modified immunogenicity as compared to the parent protein comprising the steps obtaining antibody binding peptide sequences, using the sequences to localise epitope sequences on the 3-dimensional structure of parent protein, defining an epitope area including amino acids situated within 5 ? from the epitope amino acids constituting the epitope sequence, changing one or more of the amino acids defining the epitope area of the parent protein by genetical engineering mutations of a DNA sequence encoding the parent protein, introducing the mutated DNA sequence into a suitable host, culturing said host and expressing the protein variant, and evaluating the immunogenicity of the protein variant using the parent protein as reference. The invention further relates to the protein variant and use thereof, as well as to a method for producing said protein variant.Type: ApplicationFiled: February 4, 2010Publication date: February 24, 2011Applicant: NOVOZYMES A/SInventors: Erwin Ludo Roggen, Steffen Ernst, Allan Svendsen, Esben Peter Friis, Claus Von Der Osten
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Publication number: 20090170747Abstract: The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 ?; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g.Type: ApplicationFiled: February 27, 2009Publication date: July 2, 2009Applicant: Novozymes A/SInventors: Kim Vilbour Andersen, Martin Schulein, Torben Henriksen, Lars Christensen, Bo Damgaard, Claus Von der Osten
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Publication number: 20090163400Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.Type: ApplicationFiled: August 5, 2008Publication date: June 25, 2009Applicant: Novozymes A/SInventors: Laurens Nicolaas Sierkstra, Jan Klugkist, Peter Markvardsen, Claus Von der Osten, Peter Bauditz
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Publication number: 20090143270Abstract: The present invention relates to variants (mutants) of parent Termamyl-tike alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: ApplicationFiled: January 27, 2009Publication date: June 4, 2009Applicant: Novozymes A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus von der Osten
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Patent number: 7498158Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: GrantFiled: May 15, 2002Date of Patent: March 3, 2009Assignee: Novozymes A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus Von Der Osten
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Publication number: 20080206836Abstract: The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 ?; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g.Type: ApplicationFiled: July 30, 2007Publication date: August 28, 2008Applicant: Novozymes A/SInventors: Kim Vilbour Andersen, Martin Schulein, Torben Henriksen, Lars Christiansen, Bo Damgaard, Claus Von der Osten
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Publication number: 20080167213Abstract: The present invention relates to novel subtilase variants exhibiting alterations relative to the parent subtilase in one or more properties including: Wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash compositions, including automatic dishwash compositions.Type: ApplicationFiled: February 22, 2008Publication date: July 10, 2008Applicant: Novozymes A/SInventors: Tina Sejersgard Fano, Claus Von Der Osten, Malene Kappen Krugen, Mads Norregaard-Madsen
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Patent number: 7098017Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved autoproteolytic stability in comparison to their wild type parent enzymes.Type: GrantFiled: September 6, 2001Date of Patent: August 29, 2006Assignee: Novozymes A/SInventors: Claus von der Osten, Torben Halkier, Carsten Andersen, Peter Bauditz, Peter Kamp Hansen
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Publication number: 20050079593Abstract: The present invention relates to polypeptide-polymer conjugates having added and/or removed one or more attachment groups for coupling polymeric molecules on the surface of the polypeptide structure, a method for preparing polypeptide-polymer conjugates of the invention, the use of said conjugated for reducing the immunogenicity and allergenicity and compositions comprising said conjugate.Type: ApplicationFiled: July 18, 2003Publication date: April 14, 2005Applicant: Novozymes A/SInventors: Claus von der Osten, Arne Olsen, Erwin Roggen