Abstract: A waveguide plate comprising a glass substrate (10) covered with a waveguide layer (2) has coupling grating strips following one another a distance apart, in which the coupling angle changes along parallel lines by not more than 0.1°/cm, preferably not more than 0.05°/cm. The deviation from its mean value over the total waveguide plate is not more than 0.3°, preferably not more than 0.15°. The waveguide plate is suitable as part of a microtiter plate for chemical analyses by means of fluorescence excitation. For its production, the glass substrate (1) is covered with a photoresist layer (10) and exposed to a mercury vapour lamp via a deflecting mirror through a phase mask in the vicinity of which it is arranged, for example at the Littrow angle (&thgr;L) or at a right angle, then structured by reactive ion etching and provided with the waveguide layer (2) by reactive DC magnetron sputtering, in particular pulsed DC sputtering or AC-superposed DC sputtering.