Abstract: An anaerobic reactor system that includes at least one toxicity reduction system that is configured for reducing an amount of one or more nitrogen compound and/or at least one type of recalcitrant in a substrate mixture sufficient to procedure a non-toxic substrate mixture for purposes of anaerobic digestion of the non-toxic substrate.

Abstract: An induced sludge bed anaerobic reactor system that includes at least two stages of bioreactor processing, a first-stage feeding system, a second-stage feeding system, a pH balancing system, an effluent recirculation system, a gas management system, at least one nitrogen reduction system, and a controller. In addition, the nitrogen reduction system(s) if configured for reducing an amount of one or more nitrogen compounds in a substrate mixture sufficient to procedure a non-toxic substrate mixture with respect to anaerobic digestion of the non-toxic substrate by the induced sludge bed anaerobic reactor system.

Abstract: A method for biogas production includes feeding a receiving structure with a feed containing biomass that comprises lignocellulose and directing the feed from the receiving structure into an anaerobic secretome bioreactor (ASB) reactor environment which includes a synthetic microbial community consisting of at least one selected from extremophile thermophilic anaerobic microorganisms that are essentially acidogens and acetogens, the synthetic microbial community producing a secretome of exozymes. The ASB treated biomass is further directed to an aerobic digestion (AD) reactor environment, the ASB treated biomass being pasteurized so as to be essentially free of non-thermophilic microorganisms, the ASB treated biomass comprising liquid effluent containing solubilized biomass products and metabolized biomass products, and solid effluent. Contents or heat are recycled through a conduit between the ASB reactor environment and the AD reactor environment.

Abstract: An anaerobic digestion system for producing biogas from biomass, particularly biomass that is lignocellulosic, and includes two separate and interacting anaerobic reactor environments to solubilize lignocellulosic materials and make them available for conversion to biogas.

Abstract: An induced sludge bed anaerobic reactor system that includes at least two stages of bioreactor processing, a first-stage feeding system, a second-stage feeding system, a pH balancing system, an effluent recirculation system, a gas management system, at least one nitrogen reduction system, and a controller. In addition, the nitrogen reduction system(s) if configured for reducing an amount of one or more nitrogen compounds in a substrate mixture sufficient to procedure a non-toxic substrate mixture with respect to anaerobic digestion of the non-toxic substrate by the induced sludge bed anaerobic reactor system.

Abstract: An induced sludge bed anaerobic reactor system that includes at least two stages of bioreactor processing, a first-stage feeding system, a second-stage feeding system, a pH balancing system, an effluent recirculation system, a gas management system, and a controller. In addition, any given stage of reactor processing may be comprised of a plurality of reactors that are configured to operate in parallel with each other.

Abstract: An anaerobic digestion system for producing biogas from biomass, particularly biomass that is lignocellulosic, and includes two separate and interacting anaerobic reactor environments to solubilize lignocellulosic materials and make them available for conversion to biogas.

Abstract: An induced sludge bed anaerobic reactor system that includes at least two stages of bioreactor processing, a first-stage feeding system, a second-stage feeding system, a pH balancing system, an effluent recirculation system, a gas management system, and a controller. In addition, any given stage of reactor processing may be comprised of a plurality of reactors that are configured to operate in parallel with each other.

Abstract: An induced sludge bed anaerobic reactor system that includes at least two stages of bioreactor processing, a first-stage feeding system, a second-stage feeding system, a pH balancing system, an effluent recirculation system, a gas management system, and a controller. In addition, any given stage of reactor processing may be comprised of a plurality of reactors that are configured to operate in parallel with each other.

Abstract: A system for degrading biomass with anaerobic digestion that includes a biological pretreatment with organisms that break down lignocellulosic materials before anaerobic digestion or for use as feedstock for other reactions.

Abstract: An induced sludge bed anaerobic reactor system that includes at least two stages of bioreactor processing, a first-stage feeding system, a second-stage feeding system, a pH balancing system, an effluent recirculation system, a gas management system, and a controller. In addition, any given stage of reactor processing may be comprised of a plurality of reactors that are configured to operate in parallel with each other.

Abstract: An induced sludge bed anaerobic reactor includes a vessel in which a septum is positioned to maintain solids in wastewater being treated toward a lower portion of the reactor. The septum is configured to prevent or minimize rising bacteria from exiting the vessel and to minimize clogging of the vessel. A first example septum is disclosed that is based on a linear vane design. A second example septum is disclosed that is based on a concentric vane design. A third example septum is disclosed that is based on a radial vane design that may include one or more sections. Further, septums may be layered one atop another to further minimize rising bacteria from exiting the vessel and to further minimize clogging of the vessel. Finally, a septum is attached to the inside of the vessel so as to allow it to move allowing any clogging waste floating under the septum to exit the vessel.

Abstract: An induced sludge bed anaerobic reactor includes a vessel in which a septum is positioned to maintain solids in wastewater being treated toward a lower portion of the reactor. The septum is configured to prevent or minimize rising bacteria from exiting the vessel and to minimize clogging of the vessel. A first example septum is disclosed that is based on a linear vane design. A second example septum is disclosed that is based on a concentric vane design. A third example septum is disclosed that is based on a radial vane design that may include one or more sections. Further, septums may be layered one atop another to further minimize rising bacteria from exiting the vessel and to further minimize clogging of the vessel. Finally, a septum is attached to the inside of the vessel so as to allow it to move allowing any clogging waste floating under the septum to exit the vessel.

Abstract: A machine for injecting liquids is disclosed including use of at least one air pump in fluid communication with at least one injection head having apertures for nozzles. High-pressure injectate flows from the air pump into the head, preferably through a threadlessly mounted filter located within the injection head. To further minimize contamination, fluid that enters the injection head will not contact any threading present in either the head or the nozzles. An air pressure regulator is included to ensure a steady stream of injectate of a desired pressure is delivered to an injection subject via each injection head and also helping to eliminate hesitation in delivery of the fluid by the air pump. Cleaning of the machine is simplified by moving sensitive components into a sealed enclosure, removing them from a wet working environment.

Abstract: A needleless injection device and method for injecting a food subject is disclosed in which one or more types of liquid food additive are individually and/or simultaneously delivered to a food subject within a sealed injection chamber or compartment. The needleless injection device and method of the present invention utilizes rapid, high pressure injection bursts to completely and uniformly inject the food subject, allowing different food subjects or different food additives to be sequentially injected in an efficient and instant manner. The needleless injection device and method for injecting minimizes contamination by totally enclosing the injection process within a sealed chamber, and by eliminating the need for the injection nozzles to contact, or be placed immediately adjacent to the food subject.

Abstract: An upflow anaerobic reactor includes an effluent outlet configured to direct effluent out of the reactor and a fluid-filled gas trap configured to prevent loss of biogas from the vessel. An auger assembly is operably coupled to the effluent outlet to prevent clogging of the effluent outlet by solid matter that tends to collect in the effluent outlet. The auger assembly includes at least one helical screw conveyor that rotates so as to remove solid material that collects in the effluent outlet. auger assembly can be operated on a continuous or semi-continuous basis so as to allow for continuous operation of the reactor. The auger assembly can be operated on a continuous or semi-continuous basis so as to allow for continuous operation of the reactor.

Abstract: An upflow anaerobic reactor includes an effluent outlet configured to direct effluent out of the reactor and a fluid-filled gas trap configured to prevent loss of biogas from the vessel. An auger assembly is operably coupled to the effluent outlet to prevent clogging of the effluent outlet by solid matter that tends to collect in the effluent outlet. The auger assembly includes at least one helical screw conveyor that rotates so as to remove solid material that collects in the effluent outlet. auger assembly can be operated on a continuous or semi-continuous basis so as to allow for continuous operation of the reactor. The auger assembly can be operated on a continuous or semi-continuous basis so as to allow for continuous operation of the reactor.

Abstract: An upflow bioreactor includes a vessel having an inlet and an outlet configured for upflow operation. A septum is positioned within the vessel and defines a lower chamber and an upper chamber. The septum includes an aperture that provides fluid communication between the upper chamber and lower chamber. The bioreactor also includes means for releasing pressure buildup in the lower chamber. In one configuration, the septum includes a releasable portion having an open position and a closed position. The releasable portion is configured to move to the open position in response to pressure buildup in the lower chamber. In the open position fluid communication between the lower chamber and the upper chamber is increased. Alternatively the lower chamber can include a pressure release line that is selectively actuated by pressure buildup. The pressure release mechanism can prevent the bioreactor from plugging and/or prevent catastrophic damage to the bioreactor caused by high pressures.

Abstract: Methods for steady state operation of an upflow anaerobic digester using organic matter that contains a portion of solid, non-digestible matter include (1) providing an upflow anaerobic digester, (2) providing a bacterial culture in the upflow anaerobic digester for the breakdown of organic matter, (3) introducing an influent into the upflow anaerobic digester, wherein the influent comprises a biodegradable component, a liquid component, and an amount of solid non-digestible matter, (4) operating the upflow anaerobic digester in a steady-state, (5) accumulating the solid, non-digestible matter in the upflow anaerobic digester, (6) and removing a portion of the accumulated solid, non-digestible matter from the upflow anaerobic digester through the bottom of the upflow anaerobic digester while maintaining steady-state operation of the upflow anaerobic digester.

Abstract: A method for manufacturing a biomass enriched with hydrogen-producing bacteria or spores includes providing a biomass comprising a hydrogen-producing bacteria and a competing bacteria and treating the biomass with a sufficient amount of a chemical agent for a period of time such that the treatment (i) kills, inhibits or injures substantially all of the competing bacteria and (ii) does not kill or inhibit the hydrogen-producing bacteria or causes the hydrogen-producing bacteria to form spores that are not destroyed during the chemical treatment. The method also includes digesting an enriched biomass with a non-sterile organic substrate. The enriched biomass includes hydrogen-producing bacteria or spores that are mixed in sufficient quantities with the organic substrate such that the hydrogen-producing bacteria can overcome the competing bacteria to consume the organic substrate and produce hydrogen.