Abstract: The disclosure relates in part to methods and compositions for detecting targets in a sample. Such methods and compositions can be useful for laboratory, research, and diagnostic purposes.

Abstract: The present invention provides methods, kits and compositions for the detection of an analyte. In the methods of the invention, a binding molecule coupled to a first polymerase is incubated with a modified polynucleotide template to form a copy of the modified polynucleotide template without any modified nucleotides. The unmodified copy is detected in a second amplification/primer extension reaction using a second polymerase that is unable to amplify the modified polynucleotide template. Detection of the unmodified copy is indicative of the presence and/or amount of the analyte in the sample. In place of the binding molecule coupled to a first polymerase, a pair of analyte-specific probes can also be used. The first analyte specific probe comprises a first binding moiety and a first portion of a first polymerase and the second analyte specific probe comprises a second binding moiety and a second portion of the first polymerase.

Abstract: The present invention provides methods, kits and compositions for the detection of an analyte. In the methods of the invention, a binding molecule coupled to a first polymerase is incubated with a modified polynucleotide template to form a copy of the modified polynucleotide template without any modified nucleotides. The unmodified copy is detected in a second amplification/primer extension reaction using a second polymerase that is unable to amplify the modified polynucleotide template. Detection of the unmodified copy is indicative of the presence and/or amount of the analyte in the sample. In place of the binding molecule coupled to a first polymerase, a pair of analyte-specific probes can also be used. The first analyte specific probe comprises a first binding moiety and a first portion of a first polymerase and the second analyte specific probe comprises a second binding moiety and a second portion of the first polymerase.

Abstract: The present invention provides methods, kits and compositions for the detection of an analyte. The invention is particularly suited for the detection and quantification of analytes in solution. In the methods of the invention a complex is formed between two or more analyte specific probes (ASP) and an analyte. The reactive moieties of the probes interact upon the binding of the analyte specific probes to the analyte. The reactive moieties generate a nucleic acid cleavage product which is detected and indicative of the presence of the analyte.

Abstract: Provided herein are methods, compositions and kits for detecting a target nucleic acid. A target nucleic acid can be produced, for example, by a cleavage reaction in an assay for detection of biological samples. In one aspect, a described method comprises the following steps: hybridizing the target nucleic acid having a 5? end and a 3? end to a probe to form a circular hybridization complex; forming a covalent circular target nucleic acid using the probe as a template for nucleic acid synthesis; and detecting the covalent circular target nucleic acid.

Abstract: The present invention provides protein kinase and protein phosphatase substrates, methods of detecting protein kinases and protein phosphatases, and kits for detection of protein kinases and protein phosphatases. The substrates, methods, and kits use multiple substrates that can easily be differentiated from all other substrates, thus enabling rapid and easy detection of protein kinase or protein phosphatase activities. The invention also provides methods of directionally cloning nucleic acids.

Abstract: A method and apparatus that allows information to automatically be obtained from a remote database through a user constructed interactive graphic user interface. A hyperlink is associated with an icon or image file. The hyperlink connects the icon to data stored within a database. The data represents information related to the picture represented on the icon. The icons are collected and distributed electronically for incorporation into pictures. The resulting picture allows one to access the database, which is updated with information related to the pictures represented on the icons.

Abstract: A monoclonal antibody which specifically binds with an E2A/pbx1 fusion epitope.