Patents by Inventor Cristian Ionescu-Zanetti
Cristian Ionescu-Zanetti has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11098352Abstract: The invention discloses diagnostic techniques based on single cell genomics, consisting of obtaining a blood sample, enriching a sub-population of cells present in the blood sample, sequestering individual cells or group of cells from the blood sample, obtaining sequencing data from the sequestered cells or group of cells, using genetic variant information to determine the provenance of the cells, and genetically analyzing the cells of the correct provenance to provide a diagnostic readout. Using the cell-based testing techniques of the invention, the number of false positives is greatly reduced when compared to cell-free DNA (cfDNA) based traditional testing techniques. The invention may be effectively employed for non-invasive prenatal (NIPT) diagnostics, oncological testing and other diagnostic procedures.Type: GrantFiled: November 27, 2018Date of Patent: August 24, 2021Assignee: Fluxion Biosciences, Inc.Inventors: Cristian Ionescu-Zanetti, Jeff Jensen, Michael Schwartz
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Patent number: 10294518Abstract: The invention discloses methods and apparatuses for the detection and diagnostics of genetic alterations/mutations in a target sample, which may be a solid tissue or a bodily fluid. A reference sample is also acquired, and the target and reference samples are replicated into multiple target and reference replicates. The replicates are sequenced, and the sequence data is analyzed based on a statistical test. The statistical test compares the measurements between the target and reference replicates at respective allelic indices. True positive calls are then made based on the results of the statistical testing, and the desired genetic alterations/mutations are identified at the base-pair level. The invention may be used for diagnostics related to cancer, auto-immune disease, organ transplant rejection, genetic fetal abnormalities and pathogens.Type: GrantFiled: September 16, 2016Date of Patent: May 21, 2019Assignee: Fluxion Biosciences, Inc.Inventors: Nicholas Kamps-Hughes, Cristian Ionescu-Zanetti
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Publication number: 20190093158Abstract: The invention discloses diagnostic techniques based on single cell genomics, consisting of obtaining a blood sample, enriching a sub-population of cells present in the blood sample, sequestering individual cells or group of cells from the blood sample, obtaining sequencing data from the sequestered cells or group of cells, using genetic variant information to determine the provenance of the cells, and genetically analyzing the cells of the correct provenance to provide a diagnostic readout. Using the cell-based testing techniques of the invention, the number of false positives is greatly reduced when compared to cell-free DNA (cfDNA) based traditional testing techniques. The invention may be effectively employed for non-invasive prenatal (NIPT) diagnostics, oncological testing and other diagnostic procedures.Type: ApplicationFiled: November 27, 2018Publication date: March 28, 2019Inventors: Cristian Ionescu-Zanetti, Jeff Jensen, Michael Schwartz
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Patent number: 10240186Abstract: Methods, microfluidic devices, and instruments for magnetic separation of particles from a fluid are described. Examples include microfluidic devices having a removable portion. Examples include microfluidic devices having one or more regions of reduced fluid velocity. Examples further including instruments having pneumatic interfaces. Examples further includes instruments having controllable magnets, imaging components, or combinations thereof.Type: GrantFiled: July 14, 2011Date of Patent: March 26, 2019Assignee: FLUXION BIOSCIENCES, INC.Inventors: Cristian Ionescu-Zanetti, Joshua Tanner Nevill, Michael Schwartz, Carolyn G. Conant, Roger Rudoff
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Patent number: 10167502Abstract: The invention discloses diagnostic techniques based on single cell genomics, consisting of obtaining a blood sample, enriching a sub-population of cells present in the blood sample, sequestering individual cells or group of cells from the blood sample, obtaining sequencing data from the sequestered cells or group of cells, using genetic variant information to determine the provenance of the cells, and genetically analyzing the cells of the correct provenance to provide a diagnostic readout. Using the cell-based testing techniques of the invention, the number of false positives is greatly reduced when compared to cell-free DNA (cfDNA) based traditional testing techniques. The invention may be effectively employed for non-invasive prenatal (NIPT) diagnostics, oncological testing and other diagnostic procedures.Type: GrantFiled: March 31, 2016Date of Patent: January 1, 2019Assignee: Fluxion Biosciences, Inc.Inventors: Cristian Ionescu-Zanetti, Jeff Jensen, Michael Schwartz
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Publication number: 20180080068Abstract: The invention discloses methods and apparatuses for the detection and diagnostics of genetic alterations/mutations in a target sample, which may be a solid tissue or a bodily fluid. A reference sample is also acquired, and the target and reference samples are replicated into multiple target and reference replicates. The replicates are sequenced, and the sequence data is analyzed based on a statistical test. The statistical test compares the measurements between the target and reference replicates at respective allelic indices. True positive calls are then made based on the results of the statistical testing, and the desired genetic alterations/mutations are identified at the base-pair level. The invention may be used for diagnostics related to cancer, auto-immune disease, organ transplant rejection, genetic fetal abnormalities and pathogens.Type: ApplicationFiled: September 16, 2016Publication date: March 22, 2018Inventors: Nicholas Kamps-Hughes, Cristian Ionescu-Zanetti
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Publication number: 20170268037Abstract: A method is provided including coupling magnetic beads to a population of cells in a fluid sample to form magnetically-labeled cells, magnetically separating the magnetically-labeled cells from non-magnetically-labeled cells in the fluid sample, and separating target cells from non-target cells of the magnetically-labeled cells based on a size difference between the magnetically-labeled target cells and the magnetically-labeled non-target cells. A microfluidic device is provided including a fluidic pathway traversing a magnetic isolation region and a size-based isolation region. The magnetic isolation region includes a magnet positioned to separate magnetically-labeled cells from non-magnetically labeled cells in the magnetic isolation region. The size-based isolation region includes a separator configured to separate cells less than a threshold size from cells greater than a threshold size.Type: ApplicationFiled: May 15, 2015Publication date: September 21, 2017Applicant: FLUXION BIOSCIENCES, INC.Inventors: CRISTIAN IONESCU-ZANETTI, MICHAEL SCHWARTZ, CHRISTINE TING FU, RONG ANDREA FAN
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Publication number: 20160289754Abstract: The invention discloses diagnostic techniques based on single cell genomics, consisting of obtaining a blood sample, enriching a sub-population of cells present in the blood sample, sequestering individual cells or group of cells from the blood sample, obtaining sequencing data from the sequestered cells or group of cells, using genetic variant information to determine the provenance of the cells, and genetically analyzing the cells of the correct provenance to provide a diagnostic readout. Using the cell-based testing techniques of the invention, the number of false positives is greatly reduced when compared to cell-free DNA (cfDNA) based traditional testing techniques. The invention may be effectively employed for non-invasive prenatal (NIPT) diagnostics, oncological testing and other diagnostic procedures.Type: ApplicationFiled: March 31, 2016Publication date: October 6, 2016Inventors: Cristian Ionescu-Zanetti, Jeff Jensen, Michael Schwartz
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Publication number: 20160161392Abstract: Apparatus and methods are provided for analysis of individual particles in a microfluidic device. The methods involve the immobilization of an array of particles in suspension and the application of experimental compounds. Such methods can also include electrophysiology studies including patch clamp recording, electroporation, or both in the same microfluidic device. The apparatus provided includes a microfluidic device coupled to a multi-well structure and an interface for controlling the flow of media within the microchannel device.Type: ApplicationFiled: October 20, 2015Publication date: June 9, 2016Inventors: CRISTIAN IONESCU-ZANETTI, MICHELLE KHINE, MICHAEL SCHWARTZ
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Publication number: 20130017538Abstract: Methods, microfluidic devices, and instruments for magnetic separation of particles from a fluid are described. Examples include microfluidic devices having a removable portion. Examples include microfluidic devices having one or more regions of reduced fluid velocity. Examples further including instruments having pneumatic interfaces. Examples further includes instruments having controllable magnets, imaging components, or combinations thereof.Type: ApplicationFiled: July 14, 2011Publication date: January 17, 2013Applicant: Fluxion Biosciences Inc.Inventors: Cristian Ionescu-Zanetti, Joshua Tanner Nevill, Michael Schwartz, Carolyn G. Conant, Roger Rudoff
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Patent number: 8293524Abstract: Apparatus and methods are provided for analysis of individual particles in a microfluidic device. The methods involve the immobilization of an array of particles in suspension and the application of experimental compounds. Such methods can also include electrophysiology studies including patch clamp recording, electroporation, or both in the same microfluidic device. The apparatus provided includes a microfluidic device coupled to a multi-well structure and an interface for controlling the flow of media within the microchannel device.Type: GrantFiled: March 25, 2007Date of Patent: October 23, 2012Assignee: Fluxion Biosciences Inc.Inventors: Cristian Ionescu-Zanetti, Michelle Khine, Michael Schwartz, Andrew Blatz
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Publication number: 20120264134Abstract: Apparatus and methods are provided for analysis of individual particles in a microfluidic device. The methods involve the immobilization of an array of particles in suspension and the application of experimental compounds. Such methods can also include electrophysiology studies including patch clamp recording, electroporation, or both in the same microfluidic device. The apparatus provided includes a microfluidic device coupled to a multi-well structure and an interface for controlling the flow of media within the microchannel device.Type: ApplicationFiled: April 24, 2012Publication date: October 18, 2012Applicant: Fluxion Biosciences Inc.Inventors: Cristian Ionescu-Zanetti, Michelle Khine, Michael Schwartz
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Publication number: 20120135887Abstract: Method and systems provide improved cell handling in microfluidic systems and devices using lateral cell trapping and methods of fabrication of the same that allow for selective low voltage electroporation and electrofusion.Type: ApplicationFiled: November 7, 2011Publication date: May 31, 2012Applicant: The Regents of the University of CaliforniaInventors: Luke P. Lee, Jeonggi Seo, Cristian Ionescu-Zanetti, Michelle Khine, Adrian Lau
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Patent number: 8058056Abstract: Method and systems provide improved cell handling in microfluidic systems and devices using lateral cell trapping and methods of fabrication of the same that allow for selective low voltage electroporation and electrofusion.Type: GrantFiled: August 21, 2006Date of Patent: November 15, 2011Assignee: The Regents of the University of CaliforniaInventors: Luke P. Lee, Jeonggi Seo, Cristian Ionescu-Zanetti, Michelle Khine, Adrian Lau
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Publication number: 20110045582Abstract: Method and systems that provide improved cell handling and assays in microfluidic systems and devices particularly using lateral cell trapping and methods of fabrication of the same.Type: ApplicationFiled: March 14, 2005Publication date: February 24, 2011Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Luke P. Lee, Jeonggi Seo, Cristian Ionescu-Zanetti, Michelle Khine, Poorya Sabounchi, Robin Shaw
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Publication number: 20070243523Abstract: Apparatus and methods are provided for analysis of individual particles in a microfluidic device. The methods involve the immobilization of an array of particles in suspension and the application of experimental compounds. Such methods can also include electrophysiology studies including patch clamp recording, electroporation, or both in the same microfluidic device. The apparatus provided includes a microfluidic device coupled to a multi-well structure and an interface for controlling the flow of media within the microchannel device.Type: ApplicationFiled: March 25, 2007Publication date: October 18, 2007Inventors: Cristian Ionescu-Zanetti, Michelle Khine, Michael Schwartz, Andrew Blatz
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Publication number: 20070155016Abstract: Method and systems provide improved cell handling in microfluidic systems and devices using lateral cell trapping and methods of fabrication of the same that allow for selective low voltage electroporation and electrofusion.Type: ApplicationFiled: August 21, 2006Publication date: July 5, 2007Inventors: Luke Lee, Jeonggi Seo, Cristian Ionescu-Zanetti, Michelle Khine, Adrian Lau
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Publication number: 20070028683Abstract: A pressure sensing device and method for sensing pressure utilizes a deformable cavity containing a conductive medium. Pressure changes induce deformations of the cavity, resulting in changes of conductivity, as measured by electrodes. The device may either sense pressure directly or may be used to sense the pressure in a separate cavity that is in close proximity. Since the measurements do not require electrodes in the sensing region, the device is simple to fabricate. The device also has high sensitivity, making it suitable for microfluidic or biomedical applications where a low profile and disposable device is required.Type: ApplicationFiled: June 7, 2006Publication date: February 8, 2007Inventor: Cristian Ionescu-Zanetti